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Human enzymes and proteins Neuron-specific Enolase (NSE)
/Product Detail : 8NS3 Human enzymes and proteins Neuron-specific Enolase (NSE)
Product name : Human enzymes and proteins Neuron-specific Enolase (NSE)
Catalog number : 8NS3
Supplier name : Hytest
Data sheet : Ask more or other datasheet now !
Post mortal human brain.
Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies, and syphilis.
Purity > 95 % (SDS-PAGE).
Ion-exchange chromatography and gel-filtration.
Specific activity is determined for each lot at 25 °C in 45 mM imidazole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4; 1 mM 2-PG. One unit is defined as the amount of NSE converted 1 mol of 2-phospho-D-glycerate per min at 25 °C (J.Biochem. 87, 1587-1594, 1980). Subunit composition is gamma-gamma.
Tested in ELISA and immunoblotting with anti-NSE antibodies.
Solution in 0.1 M Tris-HCl, 5 mM Mg2SO4, 0.25 M KCl, pH 5.1 adjusted with sodium phosphate.
- 20 °C
Avoid repeated freezing and thawing.
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