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Index / Hytest / Human enzymes and proteins Dephosphorylated cardiac Troponin I /Product Detail : 8T53dp Human enzymes and proteins Dephosphorylated cardiac Troponin I
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Human enzymes and proteins Dephosphorylated cardiac Troponin I

 Price: 1409   EUR
1599   USD
1093   GBP

Product name : Human enzymes and proteins Dephosphorylated cardiac Troponin I

Catalog number : 8T53dp

Quantity: 0,1mg

Availability: Yes

Supplier name : Hytest

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About this Product :

Human enzymes and proteins Dephosphorylated cardiac Troponin I Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and elisa kit to Human proteins as well as Human enzymes and proteins Dephosphorylated cardiac Troponin I.
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More Details about

Source:
Human heart tissue.
Blood samples from the tissue donors were tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.

Applications:
TnI is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium sensitivity to striated muscle actomyosin ATPase activity.
Cardiac isoform of TnI (cTnI) has two serine residues at positions 22 and 23 which could be phosphorylated by cAMP-dependent protein kinase (PKA) in response to β-adrenergic stimulation of the heart. Modification of these serines results in the changes of myocardial contractility. About 50% of cTnI purified from human cardiac tissue is mono- or biphosphorylated. cTnI purified from human cardiac muscle was dephosphorylated in vitro using alkaline phosphatase from E. coli.
TnI is suitable for use as a standard in immunoassay, immunogen for antiserum production.

Analysis:
Purity > 95 %.
TnI concentration was determined spectrophotometrically using A (0.1 %, 280 nm, 1 cm) equal to 0.42. This coefficient was calculated from the amino composition of human cTnI (FEBS Lett, 270, 57-61).
Phosphate group removal confirmed by reaction with monoclonal antibody that doesn't react with phosphorylated cTnI in ELISA and immunoblotting.

Presentation:
Frozen in 60 mM Tris-HCl, pH 7.3, 285 mM NaCl, 6 mM EGTA, 15 mM MgCl2, 3 mM DTT, 150 µM ATP, 1.5 mM CaCl2, 2.3 % glycerol and traces of (NH4)2SO4 and alkaline phosphatase.

Storage:
- 20 °C (- 70 °C for long term storage)

Other information:
Avoid repeated freezing and thawing.

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