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rabbit IgG against Clostridium histolyticum Clostripain
/Product Detail : NE041/7S rabbit IgG against Clostridium histolyticum Clostripain
Product name : rabbit IgG against Clostridium histolyticum Clostripain
Catalog number : NE041/7S
Quantity: 10 mg
Supplier name : Nordic Immunological Lab
Data sheet : Ask more or other datasheet now !
General information NORDIC IMMUNOLOGY offers over 1700 basic immunological reagents including antisera,
antibodies, immunoconjugates, purified proteins, controls and calibrators. In over 30 years,
NORDIC has established working reference standards which are periodically reviewed in the
light of current progress.
NORDENZYME™ is the name of a group of polyclonal immunologic reagents to enzymes.
Two different types of rabbit or sheep anti-enzyme products are offered: the isolated IgG
fractions (7S) of the specific antisera and the isolated antibodies (PAb) to the enzymes.
Products against some 180 enzymes are available either as 7S or PAb or in both forms. The
7S fractions are also available in biotinylated form.
The NORDENZYME™ products have been obtained by immunization with highly purified
Description IgG fraction of polyclonal rabbit antiserum to clostripain from Clostridium histolyticum
Product code NE041/7S
Biological origin Rabbit
Physical form Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
Preservative No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Immunogen Clostipain is isolated and purified from Clostridium histolyticum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Purification The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Identity & Specificity The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial
immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Cross-reactivity Cross-reactivities against enzymes of other sources may occur but have not been determined.
IgG protein concentration 10 mg/ml. No foreign proteins added.
Intended use This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical
techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of
the customer’s own choice.
Directions for use Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/30,000.
Handling The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C;
prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water,
spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or
below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down
again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS,
pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be
stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs
upon storage, this should be removed by centrifugation. It will not affect the performance of
Packing Vial with 10 mg IgG (7S) fraction, lyophilised from phosphate buffered saline.
Storage / shelf life
Lyophilised at +4°C at least 10 years
reconstituted at or below -20°C 3-5 years
reconstituted at +4°C 7 days
Caution This product should be handled by qualified persons only and appropriate precautions should
be taken in its handling and disposal, and of all associated materials. For in vitro laboratory
research purposes only.
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