Product name : rHu IL-4
Catalog number : AK8329-0002
Supplier name : Akro
Data sheet : Ask more or other datasheet now !
IL-4 has been known as a Th2 cytokine and can act on B cells, T cells, and monocytes. IL-4 exhibits antitumor activity in rodent experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses. rHu IL-4 produced in E. coli is a single, non-glycosylated polypeptide chain that contains 130 amino acids and has a molecular mass of 15 kDa.
Physical Appearance: Sterile filtered white lyophilized (freeze-dried) powder.
Source: E. coli
Formulation: Lyophilized with no additives.
Reconstitution: It is recommended to reconstitute the lyophilized product with sterile water to a concentration of 0.1 mg/ml. It can be further diluted into other aqueous solutions.
Stability: Product is very stable for up to 3 years from manufacturing date, when stored lyophilized at -20° C. Reconstituted material should be aliquoted and frozen at -20° C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Shipping Conditions: Can be shipped ambient. Product should be stored at -20° C upon receipt.
Purity greater than 98% as determined by:
Analysis by RP-HPLC.
Reducing and non-reducing SDS-PAGE.
Protein Content determined by:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
rHu IL-4 is fully biologically active when compared to a standard. The ED50 as measured by CD23 receptor binding on human peripheral macrophages is less than 1.8 ng/ml.
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
Synonyms: CYT-IL4, CYT-IL4000, BCGF, BCDF, B Cell Stimulating Factor, BSF-1, Lymphocyte Stimulatory Factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra, Ia Inducing Factor, IaIF, B Cell Differentiation Factor-epsilon, BCDF-epsilon, B Cell Differentiation Factor-gamma, BCDF-gamma, B-Cell Growth Factor-gamma, BCGF-gamma, B Cell Growth Factor-1, BCGF-1, HCGF, IgE-EF, IgG1-Enhancing Factor, IgG1-Induction Factor, LMW-BCGF, MaGEF, MCGF-2, MFF, TCGF-2
Our team will respond you as soon as possible !