Product name : rHu IL-6
Catalog number : AK8334-0005
Supplier name : Akro
Data sheet : Ask more or other datasheet now !
IL-6 is produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes. It is responsible for the stimulation of B cell differentiation, the development of megakaryocytes, production of platelets, and regulation of bone metabolism. Recombinant Human Interleukin-6 (rHu IL-6) produced in E. coli is a single, non-glycosylated polypeptide chain that contains 184 amino acids and has a molecular mass of 21 kDa.
Physical Appearance: Sterile filtered white lyophilized (freeze-dried) powder.
Source: E. coli
Formulation: Lyophilized from a sterile solution containing 10 mM Acetic acid.
Reconstitution: It is recommended to reconstitute the lyophilized product with 10 mM HCl to a concentration of 0.1 mg/ml. It can be further diluted into other aqueous solutions.
Stability: Product is very stable for up to 3 years from manufacturing date, when stored lyophilized at -20° C. Reconstituted material should be aliquoted and frozen at -20° C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Shipping Conditions: Can be shipped ambient. Product should be stored at -20° C upon receipt.
Purity greater than 95% as determined by:
Analysis by RP-HPLC.
Reducing and non-reducing SDS-PAGE.
Protein Content determined by:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Endotoxin Level: Endotoxin level as measured by LAL is <0.01 ng/ug or <0.1EU/ug.
Biological Activity: rHu IL-6 is fully biologically active when compared to a standard. The ED50, as determined by the dose-dependent stimulation of mouse 7TD1 cells, is less then 0.08 ng/ml.
PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM
Synonyms: IFN-b2, B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF, CYT-IL6000, CYT-IL6, IL-6, IL6, 26 kDa protein, B-Cell Differentiation Factors, BCDF, B-Cell Stimulating Factor, BCSF, CAT Development Factor, CDF, Colony Promoting Activity, CPA, CSF-309, Differentiation Inducing Factor, DIF, FDGI, Fibroblast-Derived Growth Inhibitor, Fibronectin Stimulating Factor, FSF, Hybridoma Growth Factor, HGF, Hepatocyte Growth Inhibitory Factor, HGI, Hybridoma/Plasmacytoma Growth Factor, HPGF, Hepatocyte Stimulating Factor, HSF, IFN-beta 2, Interleukin-hematopoietin-1, ILHP1, L-GI Factor, L-HGF, MGI-2A, Myeloma GF, Natural Killer Cell Activating Factor, NKAF, Plasmacytoma Growth Factor, PCT-GF, T-Cell Activating Factor, TAF, TPO, TSF
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