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Index / Innovative / ELISA Kits Human and Animal Rat Antithrombin III ELISA Kit /Product Detail : IRATHIIIKT ELISA Kits Human and Animal Rat Antithrombin III ELISA Kit
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#IRATHIIIKT ELISA Kits Human and Animal Rat Antithrombin III ELISA Kit

 Price: 876   EUR
994   USD
679   GBP

Product name : ELISA Kits Human and Animal Rat Antithrombin III ELISA Kit

Catalog number : IRATHIIIKT

Quantity: 1 kit

Availability: Yes

Supplier name : Innovative

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About this Product :

ELISA Kits Human and Animal Rat Antithrombin III ELISA Kit
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Description
ELISA Kits Human and Animal Rat Antithrombin III ELISA Kit is a high standard ELISA kit made with antibodies and plates of best quality as means for succesful acomplishment of your experiments and achievement of excellent and reliable results. For optimal conditions in every step of the protocol starting with the dilution of the samples, followed by incubation, blocking and washing, well designed buffers are included in the kit.

ELISA Kits Human and Animal Rat Antithrombin III ELISA Kit antibody storage Gentaur recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of ELISA Kits Human and Animal Rat Antithrombin III ELISA Kit.
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ELISA Kits Human and Animal Rat Antithrombin III ELISA Kit Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and elisa kit to Human proteins as well as ELISA Kits Human and Animal Rat Antithrombin III ELISA Kit.
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More Details about

The principle of the double antibody sandwich ELISA is represented in Figure 1.  In this assay  the Antithrombin III present in samples reacts with the anti-Antithrombin III antibodies which have been adsorbed to the surface of polystyrene microtitre wells.  After the removal of unbound proteins by washing, anti-AT3 antibodies conjugated with horseradish peroxidase (HRP), are added.  These enzyme-labeled antibodies form complexes with the previously bound  AT3.  Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB).  The quantity of bound enzyme varies directly with the concentration of AT3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of AT3 in the test sample.  The quantity of  AT3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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