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Index / Innovative / ELISA Kits Human and Animal Rat Retinol Binding Protein ELISA Kit /Product Detail : IRKTAH1188 ELISA Kits Human and Animal Rat Retinol Binding Protein ELISA Kit
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ELISA Kits Human and Animal Rat Retinol Binding Protein ELISA Kit

 Price: 905   EUR
1027   USD
702   GBP

Product name : ELISA Kits Human and Animal Rat Retinol Binding Protein ELISA Kit

Catalog number : IRKTAH1188

Quantity: 1 kit

Availability: Yes

Supplier name : Innovative

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About this Product :

ELISA Kits Human and Animal Rat Retinol Binding Protein ELISA Kit
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Description
ELISA Kits Human and Animal Rat Retinol Binding Protein ELISA Kit is a high standard ELISA kit made with antibodies and plates of best quality as means for succesful acomplishment of your experiments and achievement of excellent and reliable results. For optimal conditions in every step of the protocol starting with the dilution of the samples, followed by incubation, blocking and washing, well designed buffers are included in the kit.

ELISA Kits Human and Animal Rat Retinol Binding Protein ELISA Kit Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and elisa kit to Human proteins as well as ELISA Kits Human and Animal Rat Retinol Binding Protein ELISA Kit.
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More Details about

The principle of the double antibody sandwich ELISA is represented in Figure 1.  In this assay the Retinol Binding Protein (RBP) present in samples reacts with the anti-RBP antibodies which have been adsorbed to the surface of polystyrene microtitre wells.  After the removal of unbound proteins by washing, anti-RBP antibodies conjugated with horseradish peroxidase (HRP), are added.  These enzyme-labeled antibodies form complexes with the previously bound RBP.  Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB).  The quantity of bound enzyme varies directly with the concentration of RBP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of RBP in the test sample.  The quantity of RBP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.  

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