ELISA Kits Human and Animal Bovine Transferrin ELISA Kit opis kitu ## PRODUCT_NAME ## Warunki przechowywania: Zalecamy aby przechowywyanie odbywało się w temperaturze od 2 do 8 stopni Celsjusza. Zdecydowanie należy unikać zamrażania i rozmrażania produktu aby kit zachował swoją czułośc detekcji.
ELISA Kits Human and Animal Bovine Transferrin ELISA Kit Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and elisa kit to Human proteins as well as ELISA Kits Human and Animal Bovine Transferrin ELISA Kit. http://mybiofast.com/ver.php?search=human+&submit=Search
More Details about
The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Transferrin present in samples reacts with the anti-Transferrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Transferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Transferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Transferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Transferrin in the test sample. The quantity of Transferrin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.