ELISA Kits Human and Animal Guinea Pig IgM ELISA Kit http://mybiofast.com/ver.php?search=eia&submit=Search ELISA test expiration date We recommend storage between 3 and 7 C. For shippment the temperature can be up to 25C during 2 days. This is mostly due to the stabilyty of the standard of the ELISA Kits Human and Animal Guinea Pig IgM ELISA Kit. Alternatively we can ship the standard on blue ice.
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The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.