Product name : REASTAIN Quick Diff KIT
Catalog number : 102164
Quantity: 3 x 500 ml
Supplier name : Reagena
Data sheet : Ask more or other datasheet now !
REASTAIN Quick-Diff is an optimised colour and fixative composition for the differential staining of cellular elements in peripheral blood smears. The fast 3-minute staining procedure and the ready-to-use solutions of the REASTAIN Quick-Diff KIT make this method ideal for use in emergency departments. The kit includes three 500 ml vials; REASTAIN Quick-Diff BLUE, REASTAIN Quick-Diff RED and REASTAIN Quick-Diff FIX. The result is in differential staining of the white cells upon their cytochemical features.
EDTA blood or skin puncture sample.
Preparation of the samples
See Dacie and Lewis (1995). Preparation of applicable and good quality specimens requires special attention.
Contents of the staining reagents:
Reastain Quick-Diff Blue
Azur II 0,09 %
Glycerol 5 %
Sodiumazide <0,1 %
Reastain Quick-Diff Fix
Methanol 100 %
Methyleneblue <0,01 %
Reastain Quick-Diff Red
Eosin Y 0,12 %
Sodiumazide <0,1 %
Potassium dihydrogen phosphate/
disodium hydrogen phosphate x 2H2O 67,0 mmol/l
Reastain Quick-Diff Kit: protected from light at 2…25 °C. Unopened reagents may be used until the expiry date on the label.
Phosphate buffer: at 2…8 °C. Unopened reagents may be used until the expiry date on the label.
If the Red and Blue solutions have accidentally frozen or being kept under 0 °C, let them be in room temperature, and mix well before use.
The reagents can be applied on manual staining procedures.
Use fresh and non-expired solutions. The staining solutions in staining chambers should be changed periodically to ensure optimal staining. Prevent REASTAIN Quick-Diff FIX from evaporation. Contains methanol.
Sources of errors
Irregular distribution of the blood smear on a glass slide may result in an erroneous cell counts. Alcohols used for wiping the skin may cause hemolysis and artifacts. Do not let the specimens dry at any stage of the staining procedure. Wash properly to avoid dye artifacts. Buffered water is strongly recommended for washing. Staining result is dependent on pH. Alkaline pH increases blue and acidic pH pink or reddish tinge in the stained specimen.
When stained correctly all relevant cell types are stained individually and are identifiable by the professional.
Reastain Quick-Diff Fix: Highly flammable. Toxic by inhalation, in contact with skin and if swallowed. Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowed. Keep container tightly closed. Keep away from sources of ignition - no smoking. Wear suitable protective clothing and gloves. In case of accident or if you feel sick, seek medical advice immediately (show the label if possible).
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