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Index / Reagena / REASTAIN Quick Diff KIT /Product Detail : 102164 REASTAIN Quick Diff KIT
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REASTAIN Quick Diff KIT

 Price: 182   EUR
207   USD
141   GBP

Product name : REASTAIN Quick Diff KIT

Catalog number : 102164

Quantity: 3 x 500 ml

Availability: Yes

Supplier name : Reagena

Data sheet Data sheet : Ask more or other datasheet now !

More Details about

REASTAIN Quick-Diff is an optimised colour and fixative composition for the differential staining of cellular elements in peripheral blood smears. The fast 3-minute staining procedure and the ready-to-use solutions of the REASTAIN Quick-Diff KIT make this method ideal for use in emergency departments. The kit includes three 500 ml vials; REASTAIN Quick-Diff BLUE, REASTAIN Quick-Diff RED and REASTAIN Quick-Diff FIX. The result is in differential staining of the white cells upon their cytochemical features.

Samples
EDTA blood or skin puncture sample.

Preparation of the samples
See Dacie and Lewis (1995). Preparation of applicable and good quality specimens requires special attention.

Contents of the staining reagents:

Reastain Quick-Diff Blue
Azur II 0,09 %
Glycerol 5 %
Sodiumazide <0,1 %

Reastain Quick-Diff Fix
Methanol 100 %
Methyleneblue <0,01 %

Reastain Quick-Diff Red
Eosin Y 0,12 %
Sodiumazide <0,1 %

Phosphate buffer
Potassium dihydrogen phosphate/
disodium hydrogen phosphate x 2H2O 67,0 mmol/l

Storage
Reastain Quick-Diff Kit: protected from light at 2…25 °C. Unopened reagents may be used until the expiry date on the label.
Phosphate buffer: at 2…8 °C. Unopened reagents may be used until the expiry date on the label.
If the Red and Blue solutions have accidentally frozen or being kept under 0 °C, let them be in room temperature, and mix well before use.
Applicability
The reagents can be applied on manual staining procedures.

Attentions!
Use fresh and non-expired solutions. The staining solutions in staining chambers should be changed periodically to ensure optimal staining. Prevent REASTAIN Quick-Diff FIX from evaporation. Contains methanol.

Sources of errors
Irregular distribution of the blood smear on a glass slide may result in an erroneous cell counts. Alcohols used for wiping the skin may cause hemolysis and artifacts. Do not let the specimens dry at any stage of the staining procedure. Wash properly to avoid dye artifacts. Buffered water is strongly recommended for washing. Staining result is dependent on pH. Alkaline pH increases blue and acidic pH pink or reddish tinge in the stained specimen.

Test performance
When stained correctly all relevant cell types are stained individually and are identifiable by the professional.

Warnings
Reastain Quick-Diff Fix: Highly flammable. Toxic by inhalation, in contact with skin and if swallowed. Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowed. Keep container tightly closed. Keep away from sources of ignition - no smoking. Wear suitable protective clothing and gloves. In case of accident or if you feel sick, seek medical advice immediately (show the label if possible).

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