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Index / Bioassays / QuantiChrom™ Lactate Dehydrogenase Kit, Quantitative determination of lactate dehydrogenase LDH activity by colorimetric (565nm) method /Product Detail : DLDH-100 QuantiChrom™ Lactate Dehydrogenase Kit, Quantitative determination of lactate dehydrogenase LDH activity by colorimetric (565nm) method
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#DLDH-100 QuantiChrom™ Lactate Dehydrogenase Kit, Quantitative determination of lactate dehydrogenase LDH activity by colorimetric (565nm) method

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  Price : 332   EUR
377   USD
258   GBP
1397   Zloty
44516   JPY
2565   NOK
2749   SEK
376   CHF

Product name : QuantiChrom™ Lactate Dehydrogenase Kit, Quantitative determination of lactate dehydrogenase LDH activity by colorimetric (565nm) method

Catalog number : DLDH-100

Quantity: 100Tests

Availability: Yes

Supplier name : Bioassays

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About this Product :

QuantiChrom™ Lactate Dehydrogenase Kit, Quantitative determination of lactate dehydrogenase LDH activity by colorimetric (565nm) method antibody storage Gentaur recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of QuantiChrom™ Lactate Dehydrogenase Kit, Quantitative determination of lactate dehydrogenase LDH activity by colorimetric (565nm) method.
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DESCRIPTION
 
LACTATE DEHYDROGENASE (LDH) is an oxidoreductase which
catalyzes the interconversion of lactate and pyruvate. When disease or
injury affects tissues containing LDH, the cells release LDH into the
bloodstream, where it is identified in higher than normal levels. Therefore,
LDH is most often measured to evaluate the presence of tissue or cell
damage. The non-radioactive colorimetric LDH assay is based on the
reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic
reaction to a reduced form of MTT which exhibits an absorption maximum
at 565 nm. The intensity of the purple color formed is directly proportional to
the enzyme activity.
 
KEY FEATURES
High sensitivity and wide linear range. Use 3 μL serum or plasma
sample. The detection limit is 2 IU/L, linear up to 200 IU/L.
Homogeneous and simple procedure. Simple “mix-and-measure”
procedure allows reliable quantitation of LDH activity within 30 minutes.
Robust and amenable to HTS. All reagents are compatible with highthroughput
liquid handling instruments.
 
APPLICATIONS
Direct Assays: LDH activity in serum, plasma and other sources.
Characterization and Quality Control for LDH production.
Drug Discovery: screen and evaluation of LDH modulators.
 
KIT CONTENTS (100 tests in 96-well plates)
Substrate Buffer: 20 mL, pH 8.2
NAD Solution: 1 mL PMS Solution: 1.5 mL
MTT Solution: 1.5 mL Calibrator: 10 mL
Storage conditions. The kit is shipped at room temperature. Store all
components at -20°C upon receiving. Shelf life: 6 months after receipt.
 
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
 
MATERIALS REQUIRED, BUT NOT PROVIDED
Pipeting devices and accessories (e.g. multi-channel pipettor).
Procedure using 96-well plate:
Clear bottom 96-well plates (e.g. Corning Costar) and plate reader.
Procedure using cuvette:
Spectrophotometer and cuvets for measuring OD 565nm.

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Pathways :
WP1493: Carbon assimilation C4 pathway
WP1566: Citrate cycle (TCA cycle)
WP1567: Glycolysis and Gluconeogenesis
WP1581: Histidine metabolism
WP1612: 1,2-Dichloroethane degradation
WP1614: 1- and 2-Methylnaphthalene degradation
WP1615: 3-Chloroacrylic acid degradation
WP1621: Arginine and proline metabolism
WP1622: Ascorbate and aldarate metabolism
WP1626: Benzoate degradation via CoA ligation
WP1627: Benzoate degradation via hydroxylation
WP1628: beta-Alanine metabolism
WP1633: Bisphenol A degradation
WP1634: Butanoate metabolism
WP1647: Fatty acid biosynthesis
WP1652: Fructose and mannose metabolism
WP1654: gamma-Hexachlorocyclohexane degradation
WP1655: Geraniol degradation
WP1657: Glycerolipid metabolism
WP1665: Limonene and pinene degradation
WP1666: Linoleic acid metabolism
WP167: Eicosanoid Synthesis
WP1671: Methane metabolism
WP1673: Naphthalene and anthracene degradation
WP1680: Oxidative phosphorylation

Related Genes :
[rep 1a-1b] Replicase polyprotein 1ab (ORF1ab polyprotein) [Cleaved into: Nsp1-alpha papain-like cysteine proteinase (EC 3.4.22.-) (PCP1-alpha); Nsp1-beta papain-like cysteine proteinase (EC 3.4.22.-) (PCP1-beta); Nsp2 cysteine proteinase (EC 3.4.22.-) (CP2) (CP); Non-structural protein 3 (Nsp3); 3C-like serine proteinase (3CLSP) (EC 3.4.21.-) (Nsp4); Non-structural protein 5-6-7 (Nsp5-6-7); Non-structural protein 5 (Nsp5); Non-structural protein 6 (Nsp6); Non-structural protein 7-alpha (Nsp7-alpha); Non-structural protein 7-beta (Nsp7-beta); Non-structural protein 8 (Nsp8); RNA-directed RNA polymerase (Pol) (RdRp) (EC 2.7.7.48) (Nsp9); Helicase (Hel) (EC 3.6.4.12) (EC 3.6.4.13) (Nsp10); Non-structural protein 11 (Nsp11); Non-structural protein 12 (Nsp12)]
[rep 1a-1b] Replicase polyprotein 1ab (ORF1ab polyprotein) [Cleaved into: Nsp1-alpha papain-like cysteine proteinase (EC 3.4.22.-) (PCP1-alpha); Nsp1-beta papain-like cysteine proteinase (EC 3.4.22.-) (PCP1-beta); Nsp2 cysteine proteinase (EC 3.4.22.-) (CP2) (CP); Non-structural protein 3 (Nsp3); 3C-like serine proteinase (3CLSP) (EC 3.4.21.-) (Nsp4); Non-structural protein 5-6-7 (Nsp5-6-7); Non-structural protein 5 (Nsp5); Non-structural protein 6 (Nsp6); Non-structural protein 7-alpha (Nsp7-alpha); Non-structural protein 7-beta (Nsp7-beta); Non-structural protein 8 (Nsp8); RNA-directed RNA polymerase (Pol) (RdRp) (EC 2.7.7.48) (Nsp9); Helicase (Hel) (EC 3.6.4.12) (EC 3.6.4.13) (Nsp10); Non-structural protein 11 (Nsp11); Non-structural protein 12 (Nsp12)]
[LDHB] L-lactate dehydrogenase B chain (LDH-B) (EC 1.1.1.27) (LDH heart subunit) (LDH-H) (Renal carcinoma antigen NY-REN-46)
[Ldha Ldh-1 Ldh1] L-lactate dehydrogenase A chain (LDH-A) (EC 1.1.1.27) (LDH muscle subunit) (LDH-M)
[Ldha Ldh-1 Ldh1] L-lactate dehydrogenase A chain (LDH-A) (EC 1.1.1.27) (LDH muscle subunit) (LDH-M)
[Ldhc Ldh-3 Ldh3] L-lactate dehydrogenase C chain (LDH-C) (EC 1.1.1.27) (LDH testis subunit) (LDH-X)
[LDHA PIG19] L-lactate dehydrogenase A chain (LDH-A) (EC 1.1.1.27) (Cell proliferation-inducing gene 19 protein) (LDH muscle subunit) (LDH-M) (Renal carcinoma antigen NY-REN-59)
[Ldhb Ldh-2 Ldh2] L-lactate dehydrogenase B chain (LDH-B) (EC 1.1.1.27) (LDH heart subunit) (LDH-H)
[ldh lct] L-lactate dehydrogenase (L-LDH) (EC 1.1.1.27)
[ldh] L-lactate dehydrogenase (L-LDH) (EC 1.1.1.27)
[LDHC LDH3 LDHX] L-lactate dehydrogenase C chain (LDH-C) (EC 1.1.1.27) (Cancer/testis antigen 32) (CT32) (LDH testis subunit) (LDH-X)
[dld b2133 JW2121] Quinone-dependent D-lactate dehydrogenase (EC 1.1.5.12) ((R)-lactate:quinone 2-oxidoreductase) (D-lactate dehydrogenase) (D-LDH) (Respiratory D-lactate dehydrogenase)
[ldh] L-lactate dehydrogenase (L-LDH) (EC 1.1.1.27)
[ldh ldhL] L-lactate dehydrogenase (L-LDH) (EC 1.1.1.27)
[ldh TM_1867] L-lactate dehydrogenase (L-LDH) (EC 1.1.1.27)
[LDHA] L-lactate dehydrogenase A chain (LDH-A) (EC 1.1.1.27) (LDH muscle subunit) (LDH-M)
[Ldhb Ldh-2 Ldh2] L-lactate dehydrogenase B chain (LDH-B) (EC 1.1.1.27) (LDH heart subunit) (LDH-H)
[DLD GDH At5g06580 F15M7.11] D-lactate dehydrogenase [cytochrome], mitochondrial (AtD-LDH) (EC 1.1.2.4) (D-lactate ferricytochrome C oxidoreductase) (Glycolate dehydrogenase)
[ldha] L-lactate dehydrogenase A chain (LDH-A) (EC 1.1.1.27) (LDH-M)
[ldh2 BLLJ_1290] L-lactate dehydrogenase 2 (L-LDH 2) (EC 1.1.1.27) (FBP-dependent LDH)
[ldh lctE BSU03050] L-lactate dehydrogenase (L-LDH) (EC 1.1.1.27)
[CYB2 YML054C YM9958.08C] Cytochrome b2, mitochondrial (EC 1.1.2.3) (L-lactate dehydrogenase [Cytochrome]) (L-lactate ferricytochrome C oxidoreductase) (L-LCR)
[ldh ldhL] L-lactate dehydrogenase (L-LDH) (EC 1.1.1.27)
[Ldhc Ldh-3 Ldh3] L-lactate dehydrogenase C chain (LDH-C) (EC 1.1.1.27) (LDH testis subunit) (LDH-X)
[ldh TTHA1113] L-lactate dehydrogenase (L-LDH) (EC 1.1.1.27)
[LDHA] L-lactate dehydrogenase A chain (LDH-A) (EC 1.1.1.27) (LDH muscle subunit) (LDH-M)
[DLD2 AIP2 YDL178W] D-2-hydroxyglutarate--pyruvate transhydrogenase DLD2 (D-2HG--pyruvate transhydrogenase DLD2) (EC 1.1.99.40) (Actin-interacting protein 2) (D-lactate dehydrogenase [cytochrome] 2, mitochondrial) (EC 1.1.2.4) (D-lactate ferricytochrome C oxidoreductase) (D-LCR)
[DLD3 YEL071W] D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3 (D-2HG--pyruvate transhydrogenase DLD3) (EC 1.1.99.40) ((R)-2-hydroxyglutarate--pyruvate transhydrogenase) (D-lactate dehydrogenase [cytochrome] 3) (EC 1.1.2.4) (D-lactate ferricytochrome C oxidoreductase) (D-LCR)
[LDHB] L-lactate dehydrogenase B chain (LDH-B) (EC 1.1.1.27) (LDH heart subunit) (LDH-H)
[ldh] L-lactate dehydrogenase (L-LDH) (EC 1.1.1.27)

Bibliography :