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QuantiChrom™ Peroxidase Assay Kit, Quantitative determination of peroxidase activity by colorimetric (570nm) or fluorometric (530nm_590nm) methods
/Product Detail : DPOD-100 QuantiChrom™ Peroxidase Assay Kit, Quantitative determination of peroxidase activity by colorimetric (570nm) or fluorometric (530nm_590nm) methods
Product name : QuantiChrom™ Peroxidase Assay Kit, Quantitative determination of peroxidase activity by colorimetric (570nm) or fluorometric (530nm_590nm) methods
Catalog number : DPOD-100
Supplier name : Bioassays
Data sheet : Ask more or other datasheet now !
PEROXIDASES (EC number 1.11.1.x) catalyze the following oxidationreduction
ROOR' + electron donor (2 e-) + 2H+ → ROH + R'OH
For many peroxidases the optimal substrate is hydrogen peroxide
(H2O2), but others are more active with organic hydroperoxides such as
lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals
that are formed as byproducts during aerobic respiration. The
peroxidases represent a large family of enzymes that are found in
animals (e.g. myeloperoxidase-like enzymes), plant, fungi and bacteria
(cytochrome-c peroxidase like enzymes such a horseradish peroxidase).
Simple, direct and automation-ready procedures for determining
peroxidase activity find wide applications. BioAssay Systems' peroxidase
assay uses H2O2 and an electron donor dye that forms a pink color
during the peroxidase reaction. The optical density (570nm) or
fluorescence intensity (lexc = 530nm, lem = 590nm) is a direct measure
of the enzyme activity.
Use as little as 10 μL samples. Linear detection range: colorimetric assays
4 to 1000 IU/L, fluorimetric assays 0.8 to 25 IU/L peroxidase.
Assay Buffer (pH 7.0): 20 mL Dye Reagent: 60 μL
Stabilized H2O2: 100 μL 3% S top Reagent: 12 mL
Calibrator: 5 mL (equivalent to 1000 IU/L)
Storage conditions. The kit is shipped at room temperature. Store
Assay Buffer and Dye Reagent at -20°C, all other reagents at 4°C. Shelf
life: 12 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
Please refer to Material Safety Data Sheet for detailed information.
This assay is based on a kinetic reaction, the use of a multi-channel
pipettor for adding the Working Reagent and Stop Reagent is
MATERIALS REQUIRED, BUT NOT PROVIDED
Pipeting devices, centrifuge tubes, clear flat bottom 96/384-well
plates for colorimetric assays, black 96/384-well plate for
fluorimetric assays and plate reader.
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