Product name : DirectPCR Lysis Reagent (mouse ear)
Catalog number : [402-E]
Quantity: mouse ear (50 ml)
Supplier name : Viagen
Data sheet : Ask more or other datasheet now !
DirectPCR Lysis Reagent (mouse ear) mus musculus murine DirectPCR Lysis Reagent (mouse ear) detects proteins from variouse species most likely human.
DirectPCR® DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending components developed by scientists at Viagen Biotech Inc. allow the resulting DNA extracts to be compatible with genomic PCR for genotyping. Crude extracts of biological samples are not compatible with many molecular biology-grade reactions such as polymerase chain reaction (PCR), in part due to inhibitors contained in crude extracts. The DirectPCR reagents not only mediate the rapid lysis of biological samples but also contain inhibitors that effectively suppress the inhibitory activities of crude lysates for PCR amplification, while maximally maintaining the integrity of released genomic DNA. The patent-pending simple procedure completely eliminate any solution transfer or tube-opening steps, providing you with substancial extra time.
1. Lyse tails in DirectPCR® Lysis Reagent.
2. Incubate for 45 min at 85°C.
3. PCR genotyping with 1 ml lysates.
Detailed protocols: Tail, Ear, Yolk Sac, and Cultured cells.
DirectPCR® system offers advantages over conventional protocols that include:
· Time saving: Virtually no hands-on time. Crude tail lysates for PCR!
· Money saving:
· Safe: No organic reagents.
· Environmental: No wastes (organic reagents, tubes, tips, etc...)
· Reliable and efficient: Virtually 100% success rate with high yields.
DirectPCR Lysis Reagent (ear)
401-E 25ml (250 ear pieces).
402-E 50ml (500 ear pieces).
Our team will respond you as soon as possible !
Kits Elisa; taq POLYMERASE
|[Nr2f6 Ear-2 Ear2 Erbal2] Nuclear receptor subfamily 2 group F member 6 (COUP transcription factor 3) (COUP-TF3) (V-erbA-related protein 2) (EAR-2)|
|[Necap1] Adaptin ear-binding coat-associated protein 1 (NECAP endocytosis-associated protein 1) (NECAP-1)|
|[Nr1d1 Ear1] Nuclear receptor subfamily 1 group D member 1 (Rev-erbA-alpha) (V-erbA-related protein 1) (EAR-1)|
|[Cacna1c Cach2 Cacn2 Cacnl1a1 Cchl1a1] Voltage-dependent L-type calcium channel subunit alpha-1C (Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle) (MELC-CC) (Mouse brain class C) (MBC) (Voltage-gated calcium channel subunit alpha Cav1.2)|
|[ARP REF At2g41460 T26J13.5] DNA-(apurinic or apyrimidinic site) lyase, chloroplastic (EC 184.108.40.206) (Apurinic endonuclease-redox protein)|
|[APE2 At4g36050 T19K4.180] DNA-(apurinic or apyrimidinic site) lyase 2 (EC 220.127.116.11) (Apurinic-apyrimidinic endonuclease 2)|
|[Hps4 Le] Hermansky-Pudlak syndrome 4 protein homolog (Light-ear protein) (Le protein)|
|[Ifitm1] Interferon-induced transmembrane protein 1 (Dispanin subfamily A member 2a) (DSPA2a) (Fragilis protein 2) (Mouse ifitm-like protein 2) (Mil-2)|
|[FPG1 FPG2 At1g52500 F6D8.28] Formamidopyrimidine-DNA glycosylase (Fapy-DNA glycosylase) (EC 18.104.22.168) (EC 22.214.171.124) (DNA-(apurinic or apyrimidinic site) lyase FPG1) (Formamidopyrimidine-DNA glycosylase 1) (AtFPG-1) (Formamidopyrimidine-DNA glycosylase 2) (AtFPG-2) (Protein MutM homolog 1) (AtMMH-1) (Protein MutM homolog 2) (AtMMH-2)|
|[CPSF30 OXT6 At1g30460 F26G16.5 F26G16.6] 30-kDa cleavage and polyadenylation specificity factor 30 (EC 3.1.21.-) (Protein OXIDATIVE STRESS TOLERANT 6) (Zinc finger CCCH domain-containing protein 11) (AtC3H11)|
|[APE1L At3g48425 T29H11.60] DNA-(apurinic or apyrimidinic site) lyase (EC 3.1.-.-) (EC 126.96.36.199) (APEX1-like protein) (Apurinic-apyrimidinic endonuclease)|
|[NTH1 At2g31450 T28P16.6] Endonuclease III homolog 1, chloroplastic (AtNTH1) (EC 3.2.2.-) (EC 188.8.131.52) (Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase 1) (DNA glycosylase/AP lyase 1)|
|[NTH2 At1g05900 T20M3.18] Endonuclease III homolog 2, chloroplastic (AtNTH2) (EC 3.2.2.-) (EC 184.108.40.206) (Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase 2) (DNA glycosylase/AP lyase 2)|
|[Lsr Lisch7] Lipolysis-stimulated lipoprotein receptor (Lipolysis-stimulated receptor) (Liver-specific bHLH-Zip transcription factor) (Liver-specific gene on mouse chromosome 7 protein)|
|[Ifitm3] Interferon-induced transmembrane protein 3 (Dispanin subfamily A member 2b) (DSPA2b) (Fragilis protein) (Interferon-inducible protein 15) (Mouse ifitm-like protein 1) (Mil-1)|
|[ESD4 At4g15880 dl3980w FCAALL.406] Ubiquitin-like-specific protease ESD4 (EC 220.127.116.11) (Protein EARLY IN SHORT DAYS 4) (AtESD4)|
|[LIG6 At1g66730 F4N21.14] DNA ligase 6 (AtLIG6) (DNA ligase VI) (EC 18.104.22.168) (Ligase 1)|
|[Apex1 Ape Apex Ref1] DNA-(apurinic or apyrimidinic site) lyase (EC 3.1.-.-) (EC 22.214.171.124) (APEX nuclease) (APEN) (Apurinic-apyrimidinic endonuclease 1) (AP endonuclease 1) (REF-1) (Redox factor-1) [Cleaved into: DNA-(apurinic or apyrimidinic site) lyase, mitochondrial]|
|[Tspear Tnep1] Thrombospondin-type laminin G domain and EAR repeat-containing protein (TSP-EAR)|
|[Apex2 Ape2] DNA-(apurinic or apyrimidinic site) lyase 2 (EC 3.1.-.-) (EC 126.96.36.199) (APEX nuclease 2) (Apurinic-apyrimidinic endonuclease 2) (AP endonuclease 2)|
|[Dnajc2 Mida1 Zrf1] DnaJ homolog subfamily C member 2 (Mouse Id associate 1) (MIDA1) (Zuotin-related factor 1)|
|[Kmt2a All1 Hrx Mll Mll1] Histone-lysine N-methyltransferase 2A (Lysine N-methyltransferase 2A) (EC 188.8.131.52) (ALL-1) (Myeloid/lymphoid or mixed-lineage leukemia) (Myeloid/lymphoid or mixed-lineage leukemia protein 1) (Zinc finger protein HRX) [Cleaved into: MLL cleavage product N320 (N-terminal cleavage product of 320 kDa) (p320); MLL cleavage product C180 (C-terminal cleavage product of 180 kDa) (p180)]|
|[VTE3 APG1 IE37 At3g63410 MAA21.40] 2-methyl-6-phytyl-1,4-hydroquinone methyltransferase, chloroplastic (EC 184.108.40.2065) (37 kDa inner envelope membrane protein) (E37) (MPBQ/MSBQ methyltransferase) (Protein ALBINO OR PALE GREEN MUTANT 1) (Protein INNER ENVELOPE PROTEIN 37) (Protein VITAMIN E DEFECTIVE 3)|
|[KOR DEC KOR1 RSW2 At5g49720 K2I5.8] Endoglucanase 25 (EC 220.127.116.11) (Cellulase homolog OR16pep) (Endo-1,4-beta glucanase 25) (Protein KORRIGAN) (Protein RADIALLY SWOLLEN 2)|
|[FSD2 APG8 At5g51100 MWD22.4] Superoxide dismutase [Fe] 2, chloroplastic (EC 18.104.22.168) (Protein ALBINO OR PALE GREEN 8) (Protein FE SUPEROXIDE DISMUTASE 2)|
|[PLGG1 At1g32080 F3C3.12 T12O21.2] Plastidal glycolate/glycerate translocator 1, chloroplastic (Bacterial membrane protein LrgB-like protein) (AtLrgB)|
|[Irak1bp1 Aip70 Simpl] Interleukin-1 receptor-associated kinase 1-binding protein 1 (IRAK1-binding protein 1) (ActA-binding protein 70) (PLK-interacting protein) (Signaling molecule that associates with the mouse pelle-like kinase) (SIMPL)|
|[OR At5g61670] Protein ORANGE, chloroplastic (AtOR) (DnaJ-like cysteine-rich domain-containing protein OR)|
|[SECA1 AGY1 At4g01800 T7B11.6] Protein translocase subunit SECA1, chloroplastic (AtcpSecA) (EC 22.214.171.124) (Protein ALBINO OR GLASSY YELLOW 1)|
|[OGG1 At1g21710 F8K7.14] N-glycosylase/DNA lyase OGG1 [Includes: 8-oxoguanine DNA glycosylase 1 (AtOGG1) (EC 3.2.2.-); DNA-(apurinic or apyrimidinic site) lyase (AP lyase) (EC 126.96.36.199)]|
| Rapid zygosity determination in mice by SYBR Green real-time genomic PCR of a crude DNA solution.|
| Studies on the anti-inflammatory and related pharmacological properties of the aqueous extract of Bridelia ferruginea stem bark.|
| Potentiation of delayed-type hypersensitivity response to syngeneic tumors in mice prevaccinated with cells modified by hydrostatic pressure and crosslinking.|