antibody storage Gentaur recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of QuantiChrom™ Lactate Dehydrogenase Kit, Quantitative determination of lactate dehydrogenase LDH activity by colorimetric (565nm) method. Procedure 30 min. Kit size 100 tests. Detection limit 2 U_L..
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LACTATE DEHYDROGENASE (LDH) is an oxidoreductase which
catalyzes the interconversion of lactate and pyruvate. When disease or
injury affects tissues containing LDH, the cells release LDH into the
bloodstream, where it is identified in higher than normal levels. Therefore,
LDH is most often measured to evaluate the presence of tissue or cell
damage. The non-radioactive colorimetric LDH assay is based on the
reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic
reaction to a reduced form of MTT which exhibits an absorption maximum
at 565 nm. The intensity of the purple color formed is directly proportional to
the enzyme activity.
High sensitivity and wide linear range. Use 3 μL serum or plasma
sample. The detection limit is 2 IU/L, linear up to 200 IU/L.
Homogeneous and simple procedure. Simple “mix-and-measure”
procedure allows reliable quantitation of LDH activity within 30 minutes.
Robust and amenable to HTS. All reagents are compatible with highthroughput
liquid handling instruments.
Direct Assays: LDH activity in serum, plasma and other sources.
Characterization and Quality Control for LDH production.
Drug Discovery: screen and evaluation of LDH modulators.
KIT CONTENTS (100 tests in 96-well plates)
Substrate Buffer: 20 mL, pH 8.2
NAD Solution: 1 mL PMS Solution: 1.5 mL
MTT Solution: 1.5 mL Calibrator: 10 mL
Storage conditions. The kit is shipped at room temperature. Store all
components at -20°C upon receiving. Shelf life: 6 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
MATERIALS REQUIRED, BUT NOT PROVIDED
Pipeting devices and accessories (e.g. multi-channel pipettor).
Procedure using 96-well plate:
Clear bottom 96-well plates (e.g. Corning Costar) and plate reader.
Procedure using cuvette:
Spectrophotometer and cuvets for measuring OD 565nm.
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