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Index / Trevigen / TumorTACS Kit /Product Detail : 4815-30-K TumorTACS Kit
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  Price : 431   EUR
489   USD
334   GBP
1811   Zloty
57685   JPY
3324   NOK
3562   SEK
487   CHF

Product name : TumorTACS Kit

Catalog number : 4815-30-K

Quantity: 30 Samples

Availability: Yes

Supplier name : Trevigen

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TumorTACS Kit

Catalog # Product Name Size
4815-30-K TumorTACS Kit 30 samples
TumorTACS Kit
Catalog #: 4815-30-K

TumorTACS In Situ Apoptosis Detection Kit

TumorTACS™ is a complete reagent kit providing rapid and convenient identification of apoptosis in tumors or cancer cells. The labeling of tumor-containing specimens can be problematic due to the high levels of necrosis in these tissues. During necrosis, cessation of homeostasis, cell swelling, and nuclear membrane rupture generates highly degraded DNA that can interfere with interpretation of data. Trevigen’s kit has an optimized TUNEL-based system that preferentially labels the double-stranded DNA found in apoptotic cells. DNA fragments generated during apoptosis are end-labeled with modified nucleotides using a highly purified terminal deoxynucleotidyl transferase enzyme (TdT) in a unique non-toxic labeling buffer
supplemented with cations for apoptosis-specific labeling. The incorporated nucleotides are subsequently detected using a horseradish peroxidase conjugate. The conjugate catalyzes the conversion of diaminobenzidine (DAB) into a visible dark brown precipitate. The kit includes methyl green counterstain which is a general counterstain appropriate for a wide variety of tissues and tumor types and provides good contrast with the brown DAB in labeled cells. A DAB enhancer is also provided with the kit for the option to intensify or darken DAB staining.

TumorTACS contains all the reagents required for permeabilizing, labeling, staining, and counterstaining tumor samples, including TACS-Nuclease™ for preparing positive controls.

TumorTACS

FEATURES:

  • Fast. Requires less than 3 hours to complete.
  • Exclusive, non-toxic TACS Safe TdT buffer - sodium cacodylate free.
  • Unique buffer system produces more consistent labeling.
  • Performance tested on tumor samples.
  • Includes exclusive Cytonin permeabilization reagent.
  • Includes TACS-Nuclease solution for preparing sample-dependent positive controls.

APPLICATIONS:

  • In situ detection of apoptosis (by TUNEL) in fixed frozen, paraffin embedded, or plastic embedded cells and tissues. 
  • Assists in the identification of apoptotic morphologies.
  • Helps resolve unique problems encountered when using tissues or cells from tumors.

ITEMS NOT INCLUDED:

Reagents for dehydration and rehydration of paraffin embedded sections, H2O2, methanol, PBS buffer, mounting medium, and disposables.
 

REFERENCES:

1. Gavrieli, Y., Y. Sherman, and S.A. Ben-Sasson. 1992. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell. Biol. 119:493-501.
2. Lovelace, C.I.P., J. Zhang, P.G. Vanek, and G.B. Collier. 1996. Detecting apoptotic cells in situ. Biomedical Products 21:76-77.
3. Thiry, M. 1992. Highly sensitive immunodetection of DNA on sections with exogenous terminal deoxynucleotidyl transferase and non-isotopic nucleotide analogues. J. Histochem. Cytochem. 40:411-419.

PRODUCT CITATION:

1. Kawakubo, T., K. Okamoto, J. Iwata, M. Shin, Y. Okamoto, A. Yasukochi, K.I. Nakayama, T. Kadowaki, T. Tsukuba, and K. Yamamoto. 2007 Cathepsin E Prevents Tumor Growth and Metastasis by Catalyzing the Proteolytic Release of Soluble TRAIL from Tumor Cell Surface. Cancer Res. 67:10869–10878.


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