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Index / Trevigen / TiterTACS Colorimetric Kit /Product Detail : 4822-96-K TiterTACS Colorimetric Kit
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#4822-96-K TiterTACS Colorimetric Kit

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  Price : 442   EUR
502   USD
343   GBP
1860   Zloty
59259   JPY
3415   NOK
3659   SEK
500   CHF

Product name : TiterTACS Colorimetric Kit

Catalog number : 4822-96-K

Quantity: 96 Samples

Availability: Yes

Supplier name : Trevigen

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More Details about

4822-96-K

TiterTACS™ Colorimetric Apoptosis Detection Kit

The TiterTACS™ Colorimetric Apoptosis Detection Kit takes advantage of Trevigen’s exclusive in situ labeling technology bringing it to the 96 well microplate format for high throughput quantitative detection of apoptosis. Detection using TACS-Sapphire™, a non-toxic colorimetric substrate, allows both kinetic and endpoint readings. The reaction generates a blue product that can be measured at 630 nm, and will turn yellow after the reaction is stopped with acid
allowing endpoint reading at 450 nm.

During the process of apoptosis, DNA fragmentation occurs following the activation of endonucleases. The labeling of the 3’ ends of DNA fragments provides an easy measure of cells undergoing apoptosis. Modified nucleotides are incorporated at the 3’ ends by the activity of terminal deoxynucleotidyl transferase (TdT). These nucleotides are detected using a horseradish-peroxidase detection system and TACS-Sapphire™. TiterTACS™ can be used with suspension or monolayer cells. The kit is designed to use fixed samples, allowing you to work safely with samples that are infected with biohazardous agents. Fixed samples may be stored conveniently during time-course experiments. The TiterTACS™ Kit also provides TACS-Nuclease™ solution to generate positive controls from your own samples giving you a maximal value for the assay.

FEATURES:

  • Fast. Requires less than 4 hours to complete.
  • Exclusive, non-toxic TACS Safe™ TdT buffer - sodium cacodylate free.
  • Unique buffer system produces more consistent labeling.
  • Includes exclusive Cytonin™ permeabilization reagent.
  • Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
  • Convenient, 96 well microplate format.

APPLICATIONS:

 Identification and quantitation of apoptosis in cultured cells (by TUNEL).

ITEMS NOT INCLUDED:

Reagents for dehydration and rehydration, H2O2, methanol, PBS buffer, and disposables.
Centrifuge equipped with microplate adapters.


Kit Components:

 

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