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Index / Trevigen / VasoTACS Kit /Product Detail : 4826-30-K VasoTACS Kit
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#4826-30-K VasoTACS Kit

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  Price : 431   EUR
489   USD
334   GBP
1811   Zloty
57685   JPY
3324   NOK
3562   SEK
487   CHF

Product name : VasoTACS Kit

Catalog number : 4826-30-K

Quantity: 30 Samples

Availability: Yes

Supplier name : Trevigen

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More Details about

VasoTACS In Situ Apoptosis Detection Kit

The VasoTACS Kit was developed to provide an effective method for identifying apoptotic cells in vascular samples. This kit allows the user to successfully label apoptotic cells, including endothelial and smooth muscle cells, throughout the vascular system. Similar to the CardioTACS Kit, this kit is also based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label detection system with Red Counterstain C. Specifically, VasoTACS has been tested and optimized in order to help eliminate background and improve labeling in vascular tissue.

FEATURES:

  • Fast. Requires less than 3 hours to complete.
  • Exclusive, non-toxic TACS Safe TdT buffer - sodium cacodylate free.
  • Unique buffer system produces more consistent labeling.
  • Performance tested on vascular tissues.
  • Includes exclusive Cytonin permeabilization reagent.

APPLICATIONS:

  • In situ detection of apoptosis (by TUNEL) in fixed frozen, paraffin embedded, or plastic embedded vascular cells and tissues.
  • Assists in the identification of apoptotic morphologies.
  • Helps resolve unique problems encountered when detecting apoptotic vascular cells.

ITEMS NOT INCLUDED:

Reagents for dehydration and rehydration of paraffin embedded sections, H2O2, methanol, PBS buffer, mounting medium, and disposables.

STORAGE:

Components are stored at -20°C, 4°C and room temperature.

REFERENCES:

1. Gavrieli, Y., Y. Sherman, and S.A. Ben-Sasson. 1992. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell. Biol. 119:493-501.
2. Lovelace, C.I.P., J. Zhang, P.G. Vanek, and G.B. Collier. 1996. Detecting apoptotic cells in situ. Biomedical Products 21:76-77.

PRODUCT CITATION:

1. Skelly, C.L., A. Chandiwal, J.E. Vosicky, R.R. Weichselbaum, and B. Roizman 2007. Attenuated herpes simplex virus 1 blocks arterial apoptosis and intimal hyperplasia induced by balloon angioplasty and reduced blood flow. Proc. Natl. Acad. Sci, 104:12474-12478.

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