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Index / Trevigen / CardioTACS Kit /Product Detail : 4827-30-K CardioTACS Kit
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#4827-30-K CardioTACS Kit

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  Price : 431   EUR
489   USD
334   GBP
1811   Zloty
57685   JPY
3324   NOK
3562   SEK
487   CHF

Product name : CardioTACS Kit

Catalog number : 4827-30-K

Quantity: 30 Samples

Availability: Yes

Supplier name : Trevigen

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4827-30-K

CardioTACS Kit

CardioTACS™

The CardioTACS™ Kit was developed to provide the heart researcher with an effective method for identifying apoptotic cells in cardiac samples. The high cellularity of cardiac tissue presents problems in permeabilization, so the CardioTACS™ Kit comes with two permeabilization reagents to provide options. The kit is based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label™ detection system. Trevigen has developed an exclusive apoptosis grade TdT enzyme when coupled with the TACS Blue Label™ solution, provides labeling 20 to 50 times more sensitive than standard diaminobenzidine (DAB) labeling methods.To ensure ease of data interpretation when the numbers of apoptotic cells are low, CardioTACS™ includes Nuclear Fast Red Counterstain which provides superb contrast to the TACS Blue Label™ in apoptotic cells. In addition, the kit includes

TACS-Nuclease™ that is used to generate a positive control in your own sample. The positive control provides an internal control for permeabilization and labeling so additional optimization is nominal and the data can be interpreted with confidence. This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffer, counterstain, TACS-Nuclease™ reagent, and a protocol detailing incubation times and reagent concentrations.

CardioTACS

FEATURES:

  • Fast. Requires less than 3 hours to complete TUNEL assay.
  • Exclusive, non-toxic TACS Safe™ TdT buffer - sodium cacodylate free.
  • Unique buffer system produces more consistent labeling.
  • Performance tested on heart-derived samples.
  • Includes exclusive Cytonin™ permeabilization reagent.
  • Includes TACS-Nuclease™ solution for preparing sample-dependent positive controls.

APPLICATIONS:

  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cardiac cells and tissues.
  • Assists in the identification of apoptotic morphologies.
  • Helps resolve unique problems encountered when detecting apoptotic cardiac cells.

ITEMS NOT INCLUDED:

Reagents for dehydration and rehydration of paraffin embedded sections, H2O2, methanol, PBS buffer, mounting medium, and disposables.

REFERENCES:

1. Gavrieli, Y., Y. Sherman, and S.A. Ben-Sasson. 1992. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell. Biol. 119:493-501
2. Lovelace, C.I.P., J. Zhang, P.G. Vanek, and G.B. Collier. 1996. Detecting apoptotic cells in situ. Biomedical Products 21:76-77.
3. Thiry, M. 1992. Highly sensitive immunodetection of DNA on sections with exogenous terminal deoxynucleotidyl transferase and non-isotopic nucleotide analogues. J. Histochem. Cytochem. 40:411-419.
4. Bueno, O., L. DeWindt, K. Tymitz, S. Witt, T. Kimball, R. Klevitsky, T. Hewett, S. Jones, D. Lefer, C. Peng, R. Kitsis, and J. Molkentin. 2000. The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice. EMBO. J. 19:6341-6350.

PRODUCT CITATION:

1. Liu, X., J. A. Simpson, K.R. Brunt, C. A. Ward, S.R.R. Hall, R.T. Kinobe, V. Barrette, M.Y. Tse, S.C. Pang, A.S. Pachori, V.J. Dzau, K.O. Ogunyankin, and L.G. Melo. 2007. Preemptive heme oxygenase-1 gene delivery reveals reduced mortality and preservation of left ventricular function 1 yr after acute myocardial infarction. Am. J. Physiol. Heart. Circ. Physiol. 293: H48 - H59.


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