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NWLSS™ TAC Peroxyl Assay (Total Antioxidant Capacity)
/Product Detail : NWK-TAC01 NWLSS™ TAC Peroxyl Assay (Total Antioxidant Capacity)
Product name : NWLSS™ TAC Peroxyl Assay (Total Antioxidant Capacity)
Catalog number : NWK-TAC01
Supplier name : Northwest Life Science
Data sheet : Ask more or other datasheet now !
The NWLSS™ TAC-Peroxyl Antioxidant Capacity kit is intended for the quantitative measurement of a sample's antioxidant status against peroxyl radical challenge. This product represents a more concise tool allowing investigators to assess a sample's antioxidant capacity against a specific, biologically relevant free radical species. The peroxyl radical is involved in lipid peroxidation and other biologically significant pathways. This product is useful for testing many types of samples including blood plasma or serum, CSF, tissue homogenates, cell lysates, seminal plasma, wine, fruit juices, brewed teas and other botanical extracts. Data generated is in Trolox equivalents similar to the popular ORAC assay.
Reactive oxygen free radicals (ROS) have been implicated in more than 100 human diseases and in the aging process. Tissue damage caused by free radicals is also well documented in trauma, toxic shock and ischemia/reperfusion injury. ROS are generated endogenously by various pathways including aerobic respiration, inflammation and lipid peroxidation. Exogenously generated ROS pose an unprecedented challenge to organisms because of environmental deterioration, tobacco smoking, ionization radiation, UV-light exposure, organic solvents, anesthetics, pesticides and medications. Because of the ever present threat posed by exposure to ROS, organisms have developed powerful antioxidant defense systems to minimize or prevent possible deleterious effects of ROS exposure. Enzymatic systems such as superoxide dismutase, glutathione peroxidase and catalase aid in the decomposition of harmful radical species. Small molecules such as ascorbic acid, glutathione, uric acid, vitamin-E and CoQ-10 act as free radical scavengers. Macromolecules work to chelate metals and adsorb free radicals helping to further reduce possibly damaging effects. The overall antioxidant status is also related to other factors such as disease, life-style and an organism's stress load in general.
Numerous methods have been described to evaluate the total antioxidant capacity (TAC) of samples. These methods include scavenging assays that challenge a sample with superoxide anion radical, hydrogen peroxide, hypochlorous acid, hydroxyl radical, peroxyl radicals or peroxylnitrite. There are also methods using less biologically relevant systems such as those measuring a sample's capacity to reduce ferric ion and cuperic ion as well as those measuring a sample's scavenging ability toward 2,2-diphenyl-1picryhydrazyl (DPPH) radical and towards N,N-dimethyl- p-phenyleneamine (DMPD) radical. Since individual antioxidants differ in scavenging ability toward specif ROS species, it is important to note that TAC data generated using different assay platforms can vary to a significant degree. Because of this fact, it is best to describe TAC data in terms of a specific ROS challenge species.
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