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Index / Biolin / BIOTAQ DNA Polymerase /Product Detail : BIO-21060 BIOTAQ DNA Polymerase
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#BIO-21060 BIOTAQ DNA Polymerase

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  Price : 396   EUR
450   USD
307   GBP
1667   Zloty
53104   JPY
3060   NOK
3279   SEK
448   CHF

Product name : BIOTAQ DNA Polymerase

Catalog number : BIO-21060

Quantity: 2500 Units

Availability: Yes

Supplier name : Biolin

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More Details about

Features
 
  • Consistent results
  • Premium Taq polymerase, suited to a wide range of applications
  • Processes fragments of up to 5Kb
  • Leaves ´A´ overhang
  • Available as ready-to-use 2x reaction mixes (BioMix™ / BioMix™ Red)

 
Applications
 
  • Routine PCR applications
  • TA cloning

 
Description

BIOTAQ™ is widely used by molecular biologists that have come to depend upon the robust performance of this reagent. 

BIOTAQ is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates (Fig. 1), and is the ideal choice for most assays. BIOTAQ is a robust preparation and consistently delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors. 

BIOTAQ is supplied with 10x NH4-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl2 is provided to allow reaction conditions to be adjusted to suit the template. 

The specificity and performance of BIOTAQ can be further improved with the use of 2x PolyMate Additive which is designed for GC- or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure. 

BIOTAQ™ DNA Polymerase is purified from Thermus aquaticus.

 
Components
 
  • BIOTAQ DNA Polymerase
  • 10x NH4 Reaction Buffer
  • 50mM MgCl2 Solution

 
Unit Definition

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
 
Concentration

5u/µl
 
Storage Conditions

BIOTAQ can be stored for 12 months at -20°C.
 
Product Citations
 
  1. Amaral, I.P. & Johnston, I.A. J. Exp. Biol. 214, 2125-2139 (2011).
  2. Coutinho, C.P., et al. Infect. Immun. 79, 2950-2960 (2011).
  3. Lora, J., et al. PNAS 108, 5461-5465 (2011).
  4. Palazón, A., et al. Can. Res. 71, 801-811 (2011).
  5. del Hoyo, A. & Pedrola-Monfort, Plant Systemat. Evol. 273(3-4), 151-67 (2008).
  6. López-Lluch, G., et al. PNAS 103(6), 1768-73 (2006).
  7. Cervero, A., et al. Clin. Endocrinol. Metab. 89, 2442–51 (2004).
  8. Brigido, C., et al. Vet. Parasitol. 123(1-2), 17-23 (2004).
  9. Knight, J.C., et al. Nat. Gene. 33(4), 469-75 (2003).
  10. Goodall-Copestake, W.P., et al. Heredity104(5), 513-518. (2010)

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Pathways :
WP1438: Influenza A virus infection
WP1644: DNA replication
WP1663: Homologous recombination
WP1672: Mismatch repair
WP1693: Purine metabolism
WP1694: Pyrimidine metabolism
WP1699: RNA polymerase
WP1905: RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
WP1906: RNA Polymerase II Transcription

Related Genes :
[] Genome polyprotein [Cleaved into: P3; Protein 3AB; P2; P1; Capsid protein VP0 (VP4-VP2); Capsid protein VP4 (P1A) (Virion protein 4); Capsid protein VP2 (P1B) (Virion protein 2); Capsid protein VP3 (P1C) (Virion protein 3); Capsid protein VP1 (P1D) (Virion protein 1); Protease 2A (P2A) (EC 3.4.22.29) (Picornain 2A) (Protein 2A); Protein 2B (P2B); Protein 2C (P2C) (EC 3.6.1.15); Protein 3A (P3A); Viral protein genome-linked (VPg) (Protein 3B) (P3B); Protein 3CD (EC 3.4.22.28); Protease 3C (P3C); RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (3D polymerase) (3Dpol) (Protein 3D) (3D)]
[] Genome polyprotein [Cleaved into: P3; Protein 3AB; P2; P1; Capsid protein VP0 (VP4-VP2); Capsid protein VP4 (P1A) (Virion protein 4); Capsid protein VP2 (P1B) (Virion protein 2); Capsid protein VP3 (P1C) (Virion protein 3); Capsid protein VP1 (P1D) (Virion protein 1); Protease 2A (P2A) (EC 3.4.22.29) (Picornain 2A) (Protein 2A); Protein 2B (P2B); Protein 2C (P2C) (EC 3.6.1.15); Protein 3A (P3A); Viral protein genome-linked (VPg) (Protein 3B) (P3B); Protein 3CD (EC 3.4.22.28); Protease 3C (P3C); RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (3D polymerase) (3Dpol) (Protein 3D) (3D)]
[] Genome polyprotein [Cleaved into: P3; Protein 3AB; P2; P1; Capsid protein VP0 (VP4-VP2); Capsid protein VP4 (P1A) (Virion protein 4); Capsid protein VP2 (P1B) (Virion protein 2); Capsid protein VP3 (P1C) (Virion protein 3); Capsid protein VP1 (P1D) (Virion protein 1); Protease 2A (P2A) (EC 3.4.22.29) (Picornain 2A) (Protein 2A); Protein 2B (P2B); Protein 2C (P2C) (EC 3.6.1.15); Protein 3A (P3A); Viral protein genome-linked (VPg) (Protein 3B) (P3B); Protein 3CD (EC 3.4.22.28); Protease 3C (P3C); RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (3D polymerase) (3Dpol) (Protein 3D) (3D)]
[POLR3A] DNA-directed RNA polymerase III subunit RPC1 (RNA polymerase III subunit C1) (EC 2.7.7.6) (DNA-directed RNA polymerase III largest subunit) (DNA-directed RNA polymerase III subunit A) (RNA polymerase III 155 kDa subunit) (RPC155) (RNA polymerase III subunit C160)
[POLD1 POLD] DNA polymerase delta catalytic subunit (EC 2.7.7.7) (EC 3.1.11.-) (DNA polymerase subunit delta p125)
[POLR2A POLR2] DNA-directed RNA polymerase II subunit RPB1 (RNA polymerase II subunit B1) (EC 2.7.7.6) (DNA-directed RNA polymerase II subunit A) (DNA-directed RNA polymerase III largest subunit) (RNA-directed RNA polymerase II subunit RPB1) (EC 2.7.7.48)
[POLG2 MTPOLB] DNA polymerase subunit gamma-2, mitochondrial (EC 2.7.7.7) (DNA polymerase gamma accessory 55 kDa subunit) (p55) (Mitochondrial DNA polymerase accessory subunit) (MtPolB) (PolG-beta)
[POLG MDP1 POLG1 POLGA] DNA polymerase subunit gamma-1 (EC 2.7.7.7) (Mitochondrial DNA polymerase catalytic subunit) (PolG-alpha)
[POLD3 KIAA0039] DNA polymerase delta subunit 3 (DNA polymerase delta subunit C) (DNA polymerase delta subunit p66) (DNA polymerase delta subunit p68)
[ligD Rv0938 MTCY08D9.01c MTCY10D7.36c] Multifunctional non-homologous end joining DNA repair protein LigD (NHEJ DNA repair protein D) (Mt-Lig) (NHEJ DNA polymerase) [Includes: DNA repair polymerase (Pol) (Polymerase/primase); 3'-phosphoesterase (3'-ribonuclease/3'-phosphatase) (PE); DNA ligase (Lig) (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP])]
[Polr2a Rpii215 Rpo2-1] DNA-directed RNA polymerase II subunit RPB1 (RNA polymerase II subunit B1) (EC 2.7.7.6) (DNA-directed RNA polymerase II subunit A) (DNA-directed RNA polymerase III largest subunit)
[POLR3B] DNA-directed RNA polymerase III subunit RPC2 (RNA polymerase III subunit C2) (EC 2.7.7.6) (C128) (DNA-directed RNA polymerase III 127.6 kDa polypeptide) (DNA-directed RNA polymerase III subunit B)
[P] Protein P [Includes: DNA-directed DNA polymerase (EC 2.7.7.7); RNA-directed DNA polymerase (EC 2.7.7.49); Ribonuclease H (EC 3.1.26.4)]
[ligD MSMEG_5570 MSMEI_5419] Multifunctional non-homologous end joining protein LigD (NHEJ DNA polymerase) [Includes: DNA repair polymerase (Pol) (Polymerase/primase); 3'-phosphoesterase (3'-ribonuclease/3'-phosphatase) (PE); DNA ligase (Lig) (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP])]
[DPB2 YPR175W P9705.7] DNA polymerase epsilon subunit B (EC 2.7.7.7) (DNA polymerase II subunit 2)
[ligD PA2138] Multifunctional non-homologous end joining protein LigD (NHEJ DNA polymerase) [Includes: 3'-phosphoesterase (3'-ribonuclease/3'-phosphatase); DNA ligase D (LigD) (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP]); DNA repair polymerase (Pol) (Polymerase/primase)]
[DPB4 YDR121W] DNA polymerase epsilon subunit D (EC 2.7.7.7) (DNA polymerase II subunit D)
[POLE4] DNA polymerase epsilon subunit 4 (EC 2.7.7.7) (DNA polymerase II subunit 4) (DNA polymerase epsilon subunit p12)
[POLQ POLH] DNA polymerase theta (EC 2.7.7.7) (DNA polymerase eta)
[dnaX dnaZ dnaZX b0470 JW0459] DNA polymerase III subunit tau (EC 2.7.7.7) (DNA polymerase III subunit gamma)
[Pola1 Pola] DNA polymerase alpha catalytic subunit (EC 2.7.7.7) (DNA polymerase alpha catalytic subunit p180)
[POLR2B] DNA-directed RNA polymerase II subunit RPB2 (EC 2.7.7.6) (DNA-directed RNA polymerase II 140 kDa polypeptide) (DNA-directed RNA polymerase II subunit B) (RNA polymerase II subunit 2) (RNA polymerase II subunit B2)
[POL2 DUN2 YNL262W N0825] DNA polymerase epsilon catalytic subunit A (EC 2.7.7.7) (DNA polymerase II subunit A)
[Polq Chaos1] DNA polymerase theta (EC 2.7.7.7) (Chromosome aberrations occurring spontaneously protein 1) (DNA polymerase eta)
[POL3 CDC2 TEX1 YDL102W D2366] DNA polymerase delta catalytic subunit (EC 2.7.7.7) (DNA polymerase III)
[NRPB2 EMB1989 RP140 RPB135 RPB2 At4g21710 F17L22.170] DNA-directed RNA polymerase II subunit 2 (DNA-directed RNA polymerase II 135 kDa polypeptide) (DNA-directed RNA polymerase II subunit RPB2) (RNA polymerase II subunit 2) (RNA polymerase II subunit B2) (EC 2.7.7.6) (Protein EMBRYO DEFECTIVE 1989)
[APEX1 APE APE1 APEX APX HAP1 REF1] DNA-(apurinic or apyrimidinic site) lyase (EC 3.1.-.-) (EC 4.2.99.18) (APEX nuclease) (APEN) (Apurinic-apyrimidinic endonuclease 1) (AP endonuclease 1) (APE-1) (REF-1) (Redox factor-1) [Cleaved into: DNA-(apurinic or apyrimidinic site) lyase, mitochondrial]
[PAP2 TRF4 YOL115W HRC584 O0716] Poly(A) RNA polymerase protein 2 (EC 2.7.7.19) (DNA polymerase kappa) (DNA polymerase sigma) (Topoisomerase 1-related protein TRF4)
[dinB dinP b0231 JW0221] DNA polymerase IV (Pol IV) (EC 2.7.7.7) (Translesion synthesis polymerase IV) (TSL polymerase IV)
[POLR3G] DNA-directed RNA polymerase III subunit RPC7 (RNA polymerase III subunit C7) (DNA-directed RNA polymerase III subunit G) (RNA polymerase III 32 kDa apha subunit) (RPC32-alpha) (RNA polymerase III 32 kDa subunit) (RPC32)

Bibliography :
[24213192] Comparison of six commercially-available DNA polymerases for direct PCR.