Robust, requires minimal optimization of the reaction
Cloning techniques where high-fidelity is desirable
Amplification of difficult templates
VELOCITY is a fast thermostable enzyme possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities. VELOCITY provides high-fidelity with an error-rate of 4.4 x 10-7 combined with a high speed DNA synthesis as fast as 15s/Kb for templates of up to 5Kb and 30s/Kb for templates longer than 5Kb. The inherent high speed DNA synthesis is due to the enhanced processivity of the polymerase
VELOCITY is easy to use since it works with many different protocols and requires minimal optimization. The polymerase produces higher yields than most commercially available enzymes and generates blunt-ended amplicons. The error rate of VELOCITY DNA Polymerase (4.4 x 10-7) was determined using the rpsL fidelity assay (Lackovich et al., 2001; Fujii et al., 1999).
One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
VELOCITY DNA Polymerase can be stored for 12 months at –20°C.
On Dry Ice or Blue Ice.
Killelea, T. & Connolly, B.A. Chem. BioChem.12, 1330–1336 (2011).
Thornton, J.K., et al. PLoS ONE 6(8), e23878 (2011).
Paxton, C.W., et al. Am. J. Physiol.300, C1345-C1355 (2011).
Renaud, C., et al. J. Clin. Virol.49(1), 21-25 (2010).
Huang, X.X., et al. Am. J. Pathol.174(4), 1534–1543 (2009).