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Index / Biolin / Uracil DNA_Glycosilase (UDGase) /Product Detail : BIO-27044 Uracil DNA_Glycosilase (UDGase)
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Uracil DNA_Glycosilase (UDGase)

 Price: 70   EUR
80   USD
54   GBP

Product name : Uracil DNA_Glycosilase (UDGase)

Catalog number : BIO-27044

Quantity: 500 Units

Availability: Yes

Supplier name : Biolin

Data sheet Data sheet : Ask more or other datasheet now !

More Details about

 

Features
 
  • Removal of uracil from uracil containing DNA
  • Novel temperature sensitive mutant irreversibly inactive after heating

 
Applications
 
  • UDG treatment of uracil-containing DNA to prevent amplificaton by DNA polymerases.
  • Used to investigate features of protein – DNA interactions

 
Description

Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single– or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases). UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200mM). 

Bioline UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases. 

Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.

 
Components
 
  • Uracil DNA Glycosylase
  • 10x Reaction Buffer

 
Unit Definition

One unit is the amount of enzyme that catalyzes the release of 60µmol of uracil per minute from uracil-containing double-stranded DNA.
 
Concentration

1u/µl
 
Storage Conditions

Uracil DNA Glycosylase can be stored for 6 months at –20°C.
 
Product Citations
 
  1. Rashtchian, A., et al. PCR methods and applications 2(2), 124-130 (1992).
  2. Stivers, J.T. & Drohat, A.C. Archives of Biochemistry and Biophysics 396(1), 1-9 (2001).
  3.  

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