Novel temperature sensitive mutant irreversibly inactive after heating
UDG treatment of uracil-containing DNA to prevent amplificaton by DNA polymerases.
Used to investigate features of protein – DNA interactions
Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single– or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases). UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200mM).
Bioline UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.
Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.
Uracil DNA Glycosylase
10x Reaction Buffer
One unit is the amount of enzyme that catalyzes the release of 60µmol of uracil per minute from uracil-containing double-stranded DNA.
Uracil DNA Glycosylase can be stored for 6 months at –20°C.
Rashtchian, A., et al. PCR methods and applications 2(2), 124-130 (1992).
Stivers, J.T. & Drohat, A.C. Archives of Biochemistry and Biophysics 396(1), 1-9 (2001).