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Index / Biolin / MyTaq Red DNA Polymerase /Product Detail : BIO-21108 MyTaq Red DNA Polymerase
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#BIO-21108 MyTaq Red DNA Polymerase

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  Price : 115   EUR
131   USD
89   GBP
485   Zloty
15459   JPY
891   NOK
954   SEK
130   CHF

Product name : MyTaq Red DNA Polymerase

Catalog number : BIO-21108

Quantity: 500 Units

Availability: Yes

Supplier name : Biolin

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More Details about

Features
 
  • New generation polymerase with superior performance
  • Increased sensitivity and speed
  • Novel buffer system, including dNTPs and MgCl2
  • Robust and high yield across a wide range of templates
  • Includes red dye for direct gel loading

 
Applications
 
  • High-throughput PCR
  • Specific amplification of complex templates
  • Robust amplification of GC-rich sequences
  • TA cloning

 
Description

MyTaq™ Red DNA Polymerase is a high-performance PCR product that exhibits more robust amplification than other commonly used polymerases (Fig. 1). MyTaq Red DNA Polymerase delivers very high yield over a wide range of PCR templates, making MyTaq Red DNA Polymerase the ideal choice for most routine assays.

This new enzyme preparation from Bioline is supplied with a 5x MyTaq Red Reaction Buffer system, a proprietary formulation that saves time (Fig. 2) and delivers superior results. MyTaq buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations that eliminate the need for further optimization.

The specially designed MyTaq Red formulation does not interfere with PCR reactions and enables users to load samples directly onto a gel after the PCR without the need to add loading buffer.

MyTaq Red only requires the addition of template, primers and water, thus reducing the risk of pipetting errors and contamination, as well as shortening the set-up time.

 
Components
 
  • MyTaq DNA Polymerase
  • 5x MyTaq Reaction Buffer

 
Unit Definition

One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.
 
Concentration - 5u/µl
 
Storage Conditions

MyTaq Red DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.
 
 
Vials of 2500 units available HERE

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Pathways :
WP1438: Influenza A virus infection
WP1644: DNA replication
WP1663: Homologous recombination
WP1672: Mismatch repair
WP1693: Purine metabolism
WP1694: Pyrimidine metabolism
WP1699: RNA polymerase
WP1905: RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
WP1906: RNA Polymerase II Transcription
WP2185: Purine metabolism

Related Genes :
[RCCR ACD2 At4g37000 C7A10_360] Red chlorophyll catabolite reductase, chloroplastic (AtRCCR) (RCC reductase) (EC 1.3.7.12) (Accelerated cell death protein 2)
[ORF1] RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)
[POLG2 MTPOLB] DNA polymerase subunit gamma-2, mitochondrial (EC 2.7.7.7) (DNA polymerase gamma accessory 55 kDa subunit) (p55) (Mitochondrial DNA polymerase accessory subunit) (MtPolB) (PolG-beta)
[] RNA1 polyprotein (Genome polyprotein B) (P1) [Cleaved into: Protease cofactor (32 kDa protein); Putative helicase (EC 3.6.4.-) (58 kDa protein) (Membrane-binding protein) (NTP-binding protein) (NTB); Viral genome-linked protein (VPg); Picornain 3C-like protease (3C-like protease) (EC 3.4.22.-) (24 kDa protein); RNA-directed RNA polymerase (EC 2.7.7.48) (87 kDa protein)]
[IK RED RER] Protein Red (Cytokine IK) (IK factor) (Protein RER)
[POL1 CDC17 YNL102W N2181] DNA polymerase alpha catalytic subunit A (EC 2.7.7.7) (DNA polymerase I subunit A) (DNA polymerase alpha:primase complex p180 subunit) (DNA polymerase-primase complex p180 subunit) (Pol alpha-primase complex p180 subunit)
[POLG MDP1 POLG1 POLGA] DNA polymerase subunit gamma-1 (EC 2.7.7.7) (Mitochondrial DNA polymerase catalytic subunit) (PolG-alpha)
[POLL] DNA polymerase lambda (Pol Lambda) (EC 2.7.7.7) (EC 4.2.99.-) (DNA polymerase beta-2) (Pol beta2) (DNA polymerase kappa)
[POLE2 DPE2] DNA polymerase epsilon subunit 2 (EC 2.7.7.7) (DNA polymerase II subunit 2) (DNA polymerase epsilon subunit B)
[POL2A ABO4 EMB142 EMB2284 EMB529 ESD7 TIL1 At1g08260 T23G18.21] DNA polymerase epsilon catalytic subunit A (EC 2.7.7.7) (DNA polymerase 2 a) (AtPOL2a) (DNA polymerase II subunit a) (Protein ABA OVERLY SENSITIVE a) (Protein EARLY IN SHORT DAYS 7) (Protein EMBRYO DEFECTIVE 142) (Protein EMBRYO DEFECTIVE 2284) (Protein EMBRYO DEFECTIVE 529) (Protein TILTED 1)
[POLE3 CHRAC17] DNA polymerase epsilon subunit 3 (EC 2.7.7.7) (Arsenic-transactivated protein) (AsTP) (Chromatin accessibility complex 17 kDa protein) (CHRAC-17) (HuCHRAC17) (DNA polymerase II subunit 3) (DNA polymerase epsilon subunit p17)
[Polg2 Mtpolb] DNA polymerase subunit gamma-2, mitochondrial (EC 2.7.7.7) (DNA polymerase gamma accessory 55 kDa subunit) (p55) (Mitochondrial DNA polymerase accessory subunit) (MtPolB) (PolG-beta)
[NRPE1 DMS5 DRD3 NRPD1b RMD1 RPE1 At2g40030 T28M21.19] DNA-directed RNA polymerase V subunit 1 (DNA-directed RNA polymerase D subunit 1b) (AtNRPD1b) (Nuclear RNA polymerase D 1b) (DNA-directed RNA polymerase E subunit 1) (Nuclear RNA polymerase E 1) (EC 2.7.7.6) (Protein DEFECTIVE IN MERISTEM SILENCING 5) (Protein DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 3) (Protein RNA-DIRECTED DNA METHYLATION DEFECTIVE 1) (RNA polymerase IV subunit 1) (POL IV 1)
[POLIA At1g50840 F8A12.8] DNA polymerase I A, chloroplastic/mitochondrial (EC 2.7.7.7) (DNA polymerase PolI-like B) (AtPolI-like A) (Polymerase gamma 2) (POLGAMMA2)
[POLIB At3g20540 K10D20.8] DNA polymerase I B, chloroplastic/mitochondrial (EC 2.7.7.7) (DNA polymerase PolI-like B) (AtPolI-like B) (Polymerase gamma 1) (POLGAMMA1)
[P] Protein P [Includes: DNA-directed DNA polymerase (EC 2.7.7.7); RNA-directed DNA polymerase (EC 2.7.7.49); Ribonuclease H (EC 3.1.26.4)]
[POLE4] DNA polymerase epsilon subunit 4 (EC 2.7.7.7) (DNA polymerase II subunit 4) (DNA polymerase epsilon subunit p12)
[POLR3A] DNA-directed RNA polymerase III subunit RPC1 (RNA polymerase III subunit C1) (EC 2.7.7.6) (DNA-directed RNA polymerase III largest subunit) (DNA-directed RNA polymerase III subunit A) (RNA polymerase III 155 kDa subunit) (RPC155) (RNA polymerase III subunit C160)
[POLD3 KIAA0039] DNA polymerase delta subunit 3 (DNA polymerase delta subunit C) (DNA polymerase delta subunit p66) (DNA polymerase delta subunit p68)
[POLD1 POLD] DNA polymerase delta catalytic subunit (EC 2.7.7.7) (EC 3.1.11.-) (DNA polymerase subunit delta p125)
[POLR2A POLR2] DNA-directed RNA polymerase II subunit RPB1 (RNA polymerase II subunit B1) (EC 2.7.7.6) (DNA-directed RNA polymerase II subunit A) (DNA-directed RNA polymerase III largest subunit) (RNA-directed RNA polymerase II subunit RPB1) (EC 2.7.7.48)
[POLA1 POLA] DNA polymerase alpha catalytic subunit (EC 2.7.7.7) (DNA polymerase alpha catalytic subunit p180)
[PHYA FHY2 FRE1 HY8 At1g09570 F14J9.23] Phytochrome A (Protein ELONGATED HYPOCOTYL 8) (Protein FAR RED ELONGATED 1) (Protein FAR RED ELONGATED HYPOCOTYL 2)
[NRPB1 RPB1 RPB205 RPII At4g35800 F4B14.70] DNA-directed RNA polymerase II subunit 1 (DNA-directed RNA polymerase II subunit RPB1) (DNA polymerase II subunit B1) (EC 2.7.7.6) (DNA-directed RNA polymerase III largest subunit)
[POLQ POLH] DNA polymerase theta (EC 2.7.7.7) (DNA polymerase eta)
[dnaX dnaZ dnaZX b0470 JW0459] DNA polymerase III subunit tau (EC 2.7.7.7) (DNA polymerase III subunit gamma)
[Pola1 Pola] DNA polymerase alpha catalytic subunit (EC 2.7.7.7) (DNA polymerase alpha catalytic subunit p180)
[POLR2B] DNA-directed RNA polymerase II subunit RPB2 (EC 2.7.7.6) (DNA-directed RNA polymerase II 140 kDa polypeptide) (DNA-directed RNA polymerase II subunit B) (RNA polymerase II subunit 2) (RNA polymerase II subunit B2)
[POL2 DUN2 YNL262W N0825] DNA polymerase epsilon catalytic subunit A (EC 2.7.7.7) (DNA polymerase II subunit A)
[Pola1 Pola] DNA polymerase alpha catalytic subunit (EC 2.7.7.7) (DNA polymerase alpha catalytic subunit p180) (Fragment)

Bibliography :
[30403593] Optimization of DNA isolation method from Formalin-Fixed-Paraffin-Embedded tissues (FFPE) and comparative performance of four different Polymerase Chain Reaction (PCR) kits.