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Index / Biolin / MyTaq HS DNA Polymerase /Product Detail : BIO-21113 MyTaq HS DNA Polymerase
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#BIO-21113 MyTaq HS DNA Polymerase

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  Price : 878   EUR
997   USD
681   GBP
3689   Zloty
117517   JPY
6773   NOK
7257   SEK
993   CHF

Product name : MyTaq HS DNA Polymerase

Catalog number : BIO-21113

Quantity: 2500 Units

Availability: Yes

Supplier name : Biolin

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More Details about

"The MyTaq HS sample was so much better than anything else we have used, we have ordered more already."
 

- Roslin Institute, Scotland
 

 

Features
 
  • New generation of antibody-based hot-start polymerase
  • Highest specificity and superior performance
  • Novel buffer system, including dNTPs and MgCl2
  • Fast PCR reactions

 
Applications
 
  • High-throughput PCR
  • Assays with prolonged reaction setup on the bench or liquid handling
  • Amplification of challenging targets susceptible to mispriming
  • Amplification from challenging plant samples
  • Colony PCR
  • Multiplexing
  • Specific amplification of difficult templates (GC rich)
  • Genotyping
  • TA cloning
  • Two-Step RT-PCR

 
Description

MyTaq™ HS DNA Polymerase is a high performance PCR product powered by antibody mediated hot-start, specifically designed for fast, highly-specific, hot-start PCR. The product also has the added convenience of room temperature reaction assembly, without non-specific amplification and primer-dimer formation. This new hot-start enzyme preparation from Bioline is supplied with 5x MyTaq Reaction Buffer, a proprietary formulation containing dNTPs, MgCl2 and enhancers at optimal concentrations, removing the need for optimization and giving superior amplification (Fig. 1).
 
Components
 
  • MyTaq HS DNA Polymerase
  • 5x MyTaq Reaction Buffer

 
Unit Definition

One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.
 
Concentration

5u/µl
 
Storage Conditions

MyTaq HS DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.
 
Product Citations
 
  1. Murray, S.A., et al. Appl. Envir. Microbiol. 77, 7050-7057(2011).

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Pathways :
WP1438: Influenza A virus infection
WP1644: DNA replication
WP1663: Homologous recombination
WP1672: Mismatch repair
WP1693: Purine metabolism
WP1694: Pyrimidine metabolism
WP1699: RNA polymerase
WP1905: RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
WP1906: RNA Polymerase II Transcription
WP2185: Purine metabolism

Related Genes :
[POLL] DNA polymerase lambda (Pol Lambda) (EC 2.7.7.7) (EC 4.2.99.-) (DNA polymerase beta-2) (Pol beta2) (DNA polymerase kappa)
[POLE2 DPE2] DNA polymerase epsilon subunit 2 (EC 2.7.7.7) (DNA polymerase II subunit 2) (DNA polymerase epsilon subunit B)
[POLG2 MTPOLB] DNA polymerase subunit gamma-2, mitochondrial (EC 2.7.7.7) (DNA polymerase gamma accessory 55 kDa subunit) (p55) (Mitochondrial DNA polymerase accessory subunit) (MtPolB) (PolG-beta)
[POLE3 CHRAC17] DNA polymerase epsilon subunit 3 (EC 2.7.7.7) (Arsenic-transactivated protein) (AsTP) (Chromatin accessibility complex 17 kDa protein) (CHRAC-17) (HuCHRAC17) (DNA polymerase II subunit 3) (DNA polymerase epsilon subunit p17)
[POLR1A] DNA-directed RNA polymerase I subunit RPA1 (RNA polymerase I subunit A1) (EC 2.7.7.6) (A190) (DNA-directed RNA polymerase I largest subunit) (DNA-directed RNA polymerase I subunit A) (RNA polymerase I 194 kDa subunit) (RPA194)
[POLR3A] DNA-directed RNA polymerase III subunit RPC1 (RNA polymerase III subunit C1) (EC 2.7.7.6) (DNA-directed RNA polymerase III largest subunit) (DNA-directed RNA polymerase III subunit A) (RNA polymerase III 155 kDa subunit) (RPC155) (RNA polymerase III subunit C160)
[POLR3B] DNA-directed RNA polymerase III subunit RPC2 (RNA polymerase III subunit C2) (EC 2.7.7.6) (C128) (DNA-directed RNA polymerase III 127.6 kDa polypeptide) (DNA-directed RNA polymerase III subunit B)
[POLE4] DNA polymerase epsilon subunit 4 (EC 2.7.7.7) (DNA polymerase II subunit 4) (DNA polymerase epsilon subunit p12)
[POLD3 KIAA0039] DNA polymerase delta subunit 3 (DNA polymerase delta subunit C) (DNA polymerase delta subunit p66) (DNA polymerase delta subunit p68)
[POLD1 POLD] DNA polymerase delta catalytic subunit (EC 2.7.7.7) (EC 3.1.11.-) (DNA polymerase subunit delta p125)
[POLR2A POLR2] DNA-directed RNA polymerase II subunit RPB1 (RNA polymerase II subunit B1) (EC 2.7.7.6) (DNA-directed RNA polymerase II subunit A) (DNA-directed RNA polymerase III largest subunit) (RNA-directed RNA polymerase II subunit RPB1) (EC 2.7.7.48)
[POLA1 POLA] DNA polymerase alpha catalytic subunit (EC 2.7.7.7) (DNA polymerase alpha catalytic subunit p180)
[POLQ POLH] DNA polymerase theta (EC 2.7.7.7) (DNA polymerase eta)
[POLG MDP1 POLG1 POLGA] DNA polymerase subunit gamma-1 (EC 2.7.7.7) (Mitochondrial DNA polymerase catalytic subunit) (PolG-alpha)
[POLR2B] DNA-directed RNA polymerase II subunit RPB2 (EC 2.7.7.6) (DNA-directed RNA polymerase II 140 kDa polypeptide) (DNA-directed RNA polymerase II subunit B) (RNA polymerase II subunit 2) (RNA polymerase II subunit B2)
[POLR2C A-152E5.7] DNA-directed RNA polymerase II subunit RPB3 (RNA polymerase II subunit 3) (RNA polymerase II subunit B3) (DNA-directed RNA polymerase II 33 kDa polypeptide) (RPB33) (DNA-directed RNA polymerase II subunit C) (RPB31)
[POLE POLE1] DNA polymerase epsilon catalytic subunit A (EC 2.7.7.7) (DNA polymerase II subunit A)
[POLR3C] DNA-directed RNA polymerase III subunit RPC3 (RNA polymerase III subunit C3) (DNA-directed RNA polymerase III subunit C) (RNA polymerase III 62 kDa subunit) (RPC62)
[POLR3D BN51 BN51T] DNA-directed RNA polymerase III subunit RPC4 (RNA polymerase III subunit C4) (DNA-directed RNA polymerase III subunit D) (Protein BN51) (RNA polymerase III 47 kDa subunit) (RPC53 homolog)
[HAX1 HS1BP1] HCLS1-associated protein X-1 (HS1-associating protein X-1) (HAX-1) (HS1-binding protein 1) (HSP1BP-1)
[POLR3G] DNA-directed RNA polymerase III subunit RPC7 (RNA polymerase III subunit C7) (DNA-directed RNA polymerase III subunit G) (RNA polymerase III 32 kDa apha subunit) (RPC32-alpha) (RNA polymerase III 32 kDa subunit) (RPC32)
[POLR2J POLR2J1] DNA-directed RNA polymerase II subunit RPB11-a (RNA polymerase II subunit B11-a) (RPB11a) (DNA-directed RNA polymerase II subunit J-1) (RNA polymerase II 13.3 kDa subunit)
[POLR2D] DNA-directed RNA polymerase II subunit RPB4 (RNA polymerase II subunit B4) (DNA-directed RNA polymerase II subunit D) (RNA polymerase II 16 kDa subunit) (RPB16)
[POLR2G RPB7] DNA-directed RNA polymerase II subunit RPB7 (RNA polymerase II subunit B7) (DNA-directed RNA polymerase II subunit G) (RNA polymerase II 19 kDa subunit) (RPB19)
[POLR3F] DNA-directed RNA polymerase III subunit RPC6 (RNA polymerase III subunit C6) (DNA-directed RNA polymerase III subunit F) (RNA polymerase III 39 kDa subunit) (RPC39)
[POLR2I] DNA-directed RNA polymerase II subunit RPB9 (RNA polymerase II subunit B9) (DNA-directed RNA polymerase II subunit I) (RNA polymerase II 14.5 kDa subunit) (RPB14.5)
[POLR1E PAF53 PRAF1] DNA-directed RNA polymerase I subunit RPA49 (RNA polymerase I subunit A49) (DNA-directed RNA polymerase I subunit E) (RNA polymerase I-associated factor 1) (RNA polymerase I-associated factor 53)
[POLM polmu] DNA-directed DNA/RNA polymerase mu (Pol Mu) (EC 2.7.7.7) (Terminal transferase)
[POLR3H KIAA1665 RPC8] DNA-directed RNA polymerase III subunit RPC8 (RNA polymerase III subunit C8) (DNA-directed RNA polymerase III subunit H) (RNA polymerase III subunit 22.9 kDa subunit) (RPC22.9)
[POLR1B] DNA-directed RNA polymerase I subunit RPA2 (RNA polymerase I subunit 2) (EC 2.7.7.6) (DNA-directed RNA polymerase I 135 kDa polypeptide) (RPA135)

Bibliography :
[30403593] Optimization of DNA isolation method from Formalin-Fixed-Paraffin-Embedded tissues (FFPE) and comparative performance of four different Polymerase Chain Reaction (PCR) kits.