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Index / Biolin / MyTaq HS Red DNA Polymerase /Product Detail : BIO-21115 MyTaq HS Red DNA Polymerase
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#BIO-21115 MyTaq HS Red DNA Polymerase

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  Price : 428   EUR
485   USD
332   GBP
1797   Zloty
57255   JPY
3300   NOK
3536   SEK
484   CHF

Product name : MyTaq HS Red DNA Polymerase

Catalog number : BIO-21115

Quantity: 1000 Units

Availability: Yes

Supplier name : Biolin

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More Details about

 

"The MyTaq HS sample was so much better than anything else we have used, we have ordered more already."
 

Features

New generation of antibody-based hot-start polymerase

The highest specificity and the best performance

New buffer system including dNTPs and MgCl2

Fast PCR reactions

Red dye for loading the gel directly

 

Applications

High-throughput PCR

Assays with prolonged reaction setup on the bench or liquid handling

Amplification of challenging targets susceptible to mispriming

Colony PCR

Multiplexing

High Specificity of amplification of difficult templates ( such as GC rich)

Genotyping

TA cloning

Two-Step RT-PCR


Description

MyTaq™ HS Red DNA Polymerase is a high performance PCR reagent that is powered by antibody-mediated hot-start, specifically designed for fast, highly-specific, hot-start PCR. MyTaq HS Red does not possess polymerase activity during the reaction set-up, thus reducing non-specific amplification including primer-dimer formation. The advanced formulation of MyTaq HS Red allows fast cycling conditions, considerably reducing the reaction time without compromising PCR specificity or yield (Fig. 1). This new enzyme from Bioline is supplied with 5x MyTaq buffer system, a proprietary formulation that saves time and delivers superior results, as it contains dNTPs, MgCl2 and enhancers at optimal concentrations which removes the need for optimization.

The specially designed MyTaq Red formulation does not interfere with the PCR reaction and enables users to load samples directly onto a gel after the PCR without the need to add loading buffer.

MyTaq HS Red only requires the addition of template, primers and water, reducing the risk of pipetting errors and contamination as well as reducing the set-up time.


Components


•MyTaq HS DNA Polymerase
•5x MyTaq Reaction Buffer

 

Unit Definition

One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.


Concentration

5u/µl


Storage Conditions

MyTaq HS Red DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.

2500 Units Vilas HERE

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Pathways :
WP1438: Influenza A virus infection
WP1644: DNA replication
WP1663: Homologous recombination
WP1672: Mismatch repair
WP1693: Purine metabolism
WP1694: Pyrimidine metabolism
WP1699: RNA polymerase
WP1905: RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
WP1906: RNA Polymerase II Transcription
WP2185: Purine metabolism

Related Genes :
[POLG2 MTPOLB] DNA polymerase subunit gamma-2, mitochondrial (EC 2.7.7.7) (DNA polymerase gamma accessory 55 kDa subunit) (p55) (Mitochondrial DNA polymerase accessory subunit) (MtPolB) (PolG-beta)
[IK RED RER] Protein Red (Cytokine IK) (IK factor) (Protein RER)
[POLG MDP1 POLG1 POLGA] DNA polymerase subunit gamma-1 (EC 2.7.7.7) (Mitochondrial DNA polymerase catalytic subunit) (PolG-alpha)
[RCCR ACD2 At4g37000 C7A10_360] Red chlorophyll catabolite reductase, chloroplastic (AtRCCR) (RCC reductase) (EC 1.3.7.12) (Accelerated cell death protein 2)
[POLL] DNA polymerase lambda (Pol Lambda) (EC 2.7.7.7) (EC 4.2.99.-) (DNA polymerase beta-2) (Pol beta2) (DNA polymerase kappa)
[POLE2 DPE2] DNA polymerase epsilon subunit 2 (EC 2.7.7.7) (DNA polymerase II subunit 2) (DNA polymerase epsilon subunit B)
[POLE3 CHRAC17] DNA polymerase epsilon subunit 3 (EC 2.7.7.7) (Arsenic-transactivated protein) (AsTP) (Chromatin accessibility complex 17 kDa protein) (CHRAC-17) (HuCHRAC17) (DNA polymerase II subunit 3) (DNA polymerase epsilon subunit p17)
[POLR1A] DNA-directed RNA polymerase I subunit RPA1 (RNA polymerase I subunit A1) (EC 2.7.7.6) (A190) (DNA-directed RNA polymerase I largest subunit) (DNA-directed RNA polymerase I subunit A) (RNA polymerase I 194 kDa subunit) (RPA194)
[OPN1LW RCP] Long-wave-sensitive opsin 1 (Red cone photoreceptor pigment) (Red-sensitive opsin) (ROP)
[POLR3A] DNA-directed RNA polymerase III subunit RPC1 (RNA polymerase III subunit C1) (EC 2.7.7.6) (DNA-directed RNA polymerase III largest subunit) (DNA-directed RNA polymerase III subunit A) (RNA polymerase III 155 kDa subunit) (RPC155) (RNA polymerase III subunit C160)
[POLR3B] DNA-directed RNA polymerase III subunit RPC2 (RNA polymerase III subunit C2) (EC 2.7.7.6) (C128) (DNA-directed RNA polymerase III 127.6 kDa polypeptide) (DNA-directed RNA polymerase III subunit B)
[POLE4] DNA polymerase epsilon subunit 4 (EC 2.7.7.7) (DNA polymerase II subunit 4) (DNA polymerase epsilon subunit p12)
[POLD3 KIAA0039] DNA polymerase delta subunit 3 (DNA polymerase delta subunit C) (DNA polymerase delta subunit p66) (DNA polymerase delta subunit p68)
[POLD1 POLD] DNA polymerase delta catalytic subunit (EC 2.7.7.7) (EC 3.1.11.-) (DNA polymerase subunit delta p125)
[POLR2A POLR2] DNA-directed RNA polymerase II subunit RPB1 (RNA polymerase II subunit B1) (EC 2.7.7.6) (DNA-directed RNA polymerase II subunit A) (DNA-directed RNA polymerase III largest subunit) (RNA-directed RNA polymerase II subunit RPB1) (EC 2.7.7.48)
[POLA1 POLA] DNA polymerase alpha catalytic subunit (EC 2.7.7.7) (DNA polymerase alpha catalytic subunit p180)
[POLQ POLH] DNA polymerase theta (EC 2.7.7.7) (DNA polymerase eta)
[POLR2B] DNA-directed RNA polymerase II subunit RPB2 (EC 2.7.7.6) (DNA-directed RNA polymerase II 140 kDa polypeptide) (DNA-directed RNA polymerase II subunit B) (RNA polymerase II subunit 2) (RNA polymerase II subunit B2)
[POLR2C A-152E5.7] DNA-directed RNA polymerase II subunit RPB3 (RNA polymerase II subunit 3) (RNA polymerase II subunit B3) (DNA-directed RNA polymerase II 33 kDa polypeptide) (RPB33) (DNA-directed RNA polymerase II subunit C) (RPB31)
[POLE POLE1] DNA polymerase epsilon catalytic subunit A (EC 2.7.7.7) (DNA polymerase II subunit A)
[ORF1] RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)
[POLR3C] DNA-directed RNA polymerase III subunit RPC3 (RNA polymerase III subunit C3) (DNA-directed RNA polymerase III subunit C) (RNA polymerase III 62 kDa subunit) (RPC62)
[POLR3D BN51 BN51T] DNA-directed RNA polymerase III subunit RPC4 (RNA polymerase III subunit C4) (DNA-directed RNA polymerase III subunit D) (Protein BN51) (RNA polymerase III 47 kDa subunit) (RPC53 homolog)
[HAX1 HS1BP1] HCLS1-associated protein X-1 (HS1-associating protein X-1) (HAX-1) (HS1-binding protein 1) (HSP1BP-1)
[POLR3G] DNA-directed RNA polymerase III subunit RPC7 (RNA polymerase III subunit C7) (DNA-directed RNA polymerase III subunit G) (RNA polymerase III 32 kDa apha subunit) (RPC32-alpha) (RNA polymerase III 32 kDa subunit) (RPC32)
[] RNA1 polyprotein (Genome polyprotein B) (P1) [Cleaved into: Protease cofactor (32 kDa protein); Putative helicase (EC 3.6.4.-) (58 kDa protein) (Membrane-binding protein) (NTP-binding protein) (NTB); Viral genome-linked protein (VPg); Picornain 3C-like protease (3C-like protease) (EC 3.4.22.-) (24 kDa protein); RNA-directed RNA polymerase (EC 2.7.7.48) (87 kDa protein)]
[POLR2J POLR2J1] DNA-directed RNA polymerase II subunit RPB11-a (RNA polymerase II subunit B11-a) (RPB11a) (DNA-directed RNA polymerase II subunit J-1) (RNA polymerase II 13.3 kDa subunit)
[POLR2D] DNA-directed RNA polymerase II subunit RPB4 (RNA polymerase II subunit B4) (DNA-directed RNA polymerase II subunit D) (RNA polymerase II 16 kDa subunit) (RPB16)
[POLR2G RPB7] DNA-directed RNA polymerase II subunit RPB7 (RNA polymerase II subunit B7) (DNA-directed RNA polymerase II subunit G) (RNA polymerase II 19 kDa subunit) (RPB19)
[POLR3F] DNA-directed RNA polymerase III subunit RPC6 (RNA polymerase III subunit C6) (DNA-directed RNA polymerase III subunit F) (RNA polymerase III 39 kDa subunit) (RPC39)

Bibliography :
[30403593] Optimization of DNA isolation method from Formalin-Fixed-Paraffin-Embedded tissues (FFPE) and comparative performance of four different Polymerase Chain Reaction (PCR) kits.