PCR kit use certain PCR kits can be used for diagnostics. We supply CE approved PCR kits or research PCR kits. You can request more information about the use of Pneumocystis Carinii Real Time PCR Kit DNA I, II to
More Details about
1. Intended Use
Pneumocystis Carinii real time PCR kit is used for the detection of Pneumocystis Carinii in bronchial
lavage sample or lung section sample by using real time PCR systems.
Pneumocystis pneumonia (PCP) is a form of pneumonia caused by the yeast-like fungus, Pneumocystis
jirovecii . The causal agent was originally described as a protozoan and spelled P. jiroveci and prior to
then was classified as a form of Pneumocystis carinii, a name still in common usage. These names are
discussed below. As a result, Pneumocystis pneumonia (PCP) has also been known as Pneumocystis
jirovecii pneumonia and as Pneumocystis carinii pneumonia.
It is relatively rare in people with normal immune systems but common among people with weakened
immune systems, such as premature or severely malnourished children, the elderly, and especially AIDS
patients, in whom it is most commonly observed today. PCP can also develop in patients who are
taking immunosuppressant medications (e.g. patients who have undergone solid organ transplantation)
and in patients who have undergone bone marrow transplantation.
3. Principle of Real-Time PCR
The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR
reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the
quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent
signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system.
The PCR cycle at which an increase in the fluorescence signal is detected initially (Ct) is proportional to
the amount of the specific PCR product. Monitoring the fluorescence intensities during Real Time
allows the detection of the accumulating product without having to re-open the reaction tube after the
4. Product Description
Pneumocystis Carinii real time PCR kit contains a specific ready-to-use system for the detection of the
Pneumocystis Carinii by polymerase chain reaction in the real-time PCR system. The master contains
reagents and enzymes for the specific amplification of the Pneumocystis Carinii DNA. Fluorescence is
emitted and measured by the real time systems´ optical unit. The detection of amplified Pneumocystis
Carinii DNA fragment is performed in fluorimeter channel FAM with the fluorescent quencher BHQ1.
DNA extraction buffer is available in the kit and bronchial lavage sample or lung section sample is used
for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition
by measuring the VIC/JOE fluorescence of the internal control (IC). An external positive control
copies/ml) contained, allows the determination of the gene load. For further information, please
refer to section 10.3 Quantitation.
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