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Index / Arbor / Cyclic AMP Total EIA Kit (Five Plate) /Product Detail : K019-H5 Cyclic AMP Total EIA Kit (Five Plate)
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#K019-H5 Cyclic AMP Total EIA Kit (Five Plate)

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  Price : 1446   EUR
1641   USD
1122   GBP
6075   Zloty
193525   JPY
11154   NOK
11951   SEK
1635   CHF

Product name : Cyclic AMP Total EIA Kit (Five Plate)

Catalog number : K019-H5

Quantity: 5x96 well plate

Availability: Yes

Supplier name : Arbor

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FEATURES

  • Multi Species
  • USE - Measure cAMP in 2.5 Hours
  • SAMPLE - Lysates, Urine, Plasma, Saliva, Tissue
  • SAMPLES/KIT - 39 or 231 in Duplicate
  • SENSITIVITY - Measure < 5 fmol cAMP in sample
  • STABILITY - Stable 4˚C Liquid Reagents

DESCRIPTION

The DetectX® Cyclic AMP (cAMP) Direct Immunoassay kit is designed to quantitatively measure cAMP present in lysed cells, tissue, plasma, urine, saliva and culture media samples. The kit is unique in that all samples and standards are diluted into an acidic Sample Diluent which contains cAMP stabilizers and is designed to lyse cells. A cAMP standard is provided to generate a standard curve for the assay. A special Plate Primer is added to all the wells and standards or samples in Sample Diluent are pipetted into the primed clear microtiter plate. A cAMP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to cAMP. After 2 hours, the plate is washed and substrate is added. The substrate reacts with the bound cAMP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is read. An optional Acetylation Format allows super low concentrations of cAMP to be measured.

Adenosine-3’,5’-cyclic monophosphate, or cyclic AMP (cAMP), is one of the most important second messengers and a key intracellular regulator. Discovered by Sutherland and Rall in 1957, it functions as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Adenylate cyclase is activated by the hormones glucagon and adrenaline and by G protein. Liver adenylate cyclase responds more strongly to glucagon, and muscle adenylate cyclase responds more strongly to adrenaline. cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterase. In the Human Metabolome Database there are 166 metabolic enzymes listed that convert cAMP.

Cyclic AMP EIA Standard Curve

 

http://www.ncbi.nlm.nih.gov/pubmed/21938401
http://www.ncbi.nlm.nih.gov/pubmed/21835143
http://www.ncbi.nlm.nih.gov/pubmed/22158563
http://www.ncbi.nlm.nih.gov/pubmed/22046414
http://www.ncbi.nlm.nih.gov/pubmed/22661691
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0043091
http://www.ncbi.nlm.nih.gov/pubmed/22046243
http://www.ncbi.nlm.nih.gov/pubmed/22371971
 

 

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