Product name : Lenti_III_PGK Yamanaka Set
Catalog number : G359
Quantity: 5 x 200uL, 10^7 cfu/mL
Supplier name : ABM
Data sheet : Ask more or other datasheet now !
|Vector||Refer to Individual Virus Pages|
|Plasmid Size||Refer to individual viruses.|
|Gene||Oct4, Sox2, KLF4, Myc and GFP control virus.|
|Accession #||Q01860, P48431, O43474 and P01106|
|Description||Five Lenti-III-PGK viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) and a GFP control. Purified ready-to-use high titer lentivirus at 1x107 cfu/mL|
|Functions||Each individual recombinant lentivirus is provided as a VSV-G pseudotyped and concentrated virus stock capable of infecting both dividing and non-dividing cells. The expression of the four transcription factors provided in this set in combinantion with each other has been shown to reprogram adult human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs).|
|Quality Control||Viral titer (cfu/mL) is determined by quantitative RT-PCR of viral stock preparation. Transcription factor protein expression are verified by immunocytochemistry and Western Blot analysis of transduced 293 cells. All of ABMâ€™s viral preparations are tested to be free of bacteria and other microbials.|
|Shipping||On Dry Ice|
Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72.
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10.
Our team will respond you as soon as possible !