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Index / ABM / Lenti_III_PGK Yamanaka Set /Product Detail : G359 Lenti_III_PGK Yamanaka Set
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#G359

Lenti_III_PGK Yamanaka Set

 Price: 1873   EUR
2126   USD
1453   GBP

Product name : Lenti_III_PGK Yamanaka Set

Catalog number : G359

Quantity: 5 x 200uL, 10^7 cfu/mL

Availability: Yes

Supplier name : ABM

Data sheet Data sheet : Ask more or other datasheet now !

More Details about

Vector Refer to Individual Virus Pages
Plasmid Size Refer to individual viruses.
Promoter PGK
Gene Oct4, Sox2, KLF4, Myc and GFP control virus.
Organism Human
Accession # Q01860, P48431, O43474 and P01106
Description Five Lenti-III-PGK viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) and a GFP control. Purified ready-to-use high titer lentivirus at 1x107 cfu/mL
Functions Each individual recombinant lentivirus is provided as a VSV-G pseudotyped and concentrated virus stock capable of infecting both dividing and non-dividing cells. The expression of the four transcription factors provided in this set in combinantion with each other has been shown to reprogram adult human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs).
Quality Control Viral titer (cfu/mL) is determined by quantitative RT-PCR of viral stock preparation. Transcription factor protein expression are verified by immunocytochemistry and Western Blot analysis of transduced 293 cells. All of ABM’s viral preparations are tested to be free of bacteria and other microbials.
Titer 1x107 cfu/mL
Storage -70°C
Shipping On Dry Ice
References Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72.

Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10.

 

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