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Index / Biovis / Caspase_3, human recombinant /Product Detail : 1083-25 Caspase_3, human recombinant
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#1083-25 Caspase_3, human recombinant

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  Price : 194   EUR
221   USD
151   GBP
817   Zloty
26051   JPY
1501   NOK
1608   SEK
220   CHF

Product name : Caspase_3, human recombinant

Catalog number : 1083-25

Quantity: 25 units

Availability: Yes

Supplier name : Biovis

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About this Product :

Caspase_3, human recombinant recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. Please read carefully the data sheet of the Caspase_3, human recombinant.
http://mybiofast.com/ver.php?search=rec.&submit=Search

Caspase_3, human recombinant Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Caspase_3, human recombinant.
http://mybiofast.com/ver.php?search=human+&submit=Search

More Details about

FORM: Lyophilized powder
RECONSTITUTION: Reconstitute in PBS containing 15% glycerol.


STORAGE CONDITIONS:
The lyophilized caspase-3 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.


DESCRIPTION:
Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspasefamily of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme.
During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme (Full length gene Accession No. NP_004337) . The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat.#1008-200) or Ac-DEVD-AFC (1007-200).


APPLICATIONS AND USAGE:
Active caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays and Western blot analysis.
For a complete caspase-3 assay protocol, please refer to BioVision’s Caspase-3/CPP32 Fluorometric or Colorimetric Assay Kits (Cat.#: K105 and K106).


For Research Use Only! Not to be used in humans.

 

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