Product name : Caspase_3, mouse recombinant
Catalog number : 1183-25
Quantity: 25 units
Supplier name : Biovis
Data sheet : Ask more or other datasheet now !
Caspase_3, mouse recombinant mus musculus murine Caspase_3, mouse recombinant detects proteins from variouse species most likely human.
One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 300,000 units/mg
The lyophilized caspase-3 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.
Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspasefamily of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme.
During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat. #1008-200) or Ac-DEVD-AFC (1007-200).
APPLICATIONS AND USAGE:
Active caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase-3 assay protocol, please refer to BioVision’s Caspase-3/CPP32 Fluorometric or Colorimetric Assay Kits (Cat.#: K105 and K106).
Apoptosis Detection Kits & Reagents
Cell Fractionation System
Cell Proliferation & Senescence
Cell Damage & Repair
Adipocyte & Lipid Transfer
Molecular Biology & Reporter Assays
Growth Factors and Cytokines
Monoclonal and Polyclonal Antibodies
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Kits Elisa; taq POLYMERASE
|[Casp4 Casp11 Caspl Ich3] Caspase-4 (CASP-4) (EC 18.104.22.168) (Caspase-11) (CASP-11) (Protease ICH-3) [Cleaved into: Caspase-4 subunit p10; Caspase-4 subunit p20]|
|[Casp3 Cpp32] Caspase-3 (CASP-3) (EC 22.214.171.124) (Apopain) (Cysteine protease CPP32) (CPP-32) (LICE) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]|
|[Casp2 Ich1 Nedd-2 Nedd2] Caspase-2 (CASP-2) (EC 126.96.36.199) (Neural precursor cell expressed developmentally down-regulated protein 2) (NEDD-2) (Protease ICH-1) [Cleaved into: Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12]|
|[Casp7 Lice2 Mch3] Caspase-7 (CASP-7) (EC 188.8.131.52) (Apoptotic protease Mch-3) (Cysteine protease LICE2) [Cleaved into: Caspase-7 subunit p20; Caspase-7 subunit p11]|
|[Casp6] Caspase-6 (CASP-6) (EC 184.108.40.206) (Apoptotic protease Mch-2) [Cleaved into: Caspase-6 subunit p18; Caspase-6 subunit p11]|
|[Casp1 Il1bc] Caspase-1 (CASP-1) (EC 220.127.116.11) (Interleukin-1 beta convertase) (IL-1BC) (Interleukin-1 beta-converting enzyme) (ICE) (IL-1 beta-converting enzyme) (p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10]|
|[Casp14] Caspase-14 (CASP-14) (EC 3.4.22.-) (Mini-ICE) (MICE) [Cleaved into: Caspase-14 subunit p17, mature form; Caspase-14 subunit p10, mature form; Caspase-14 subunit p20, intermediate form; Caspase-14 subunit p8, intermediate form]|
|[Casp9 Mch6] Caspase-9 (CASP-9) (EC 18.104.22.168) (Apoptotic protease Mch-6) (Apoptotic protease-activating factor 3) (APAF-3) (ICE-like apoptotic protease 6) (ICE-LAP6) [Cleaved into: Caspase-9 subunit p35; Caspase-9 subunit p10]|
|[Casp8] Caspase-8 (CASP-8) (EC 22.214.171.124) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]|
|[Casp12] Caspase-12 (CASP-12) (EC 3.4.22.-)|
|[Cflar Cash] CASP8 and FADD-like apoptosis regulator (Caspase homolog) (CASH) (Caspase-eight-related protein) (Casper) (Caspase-like apoptosis regulatory protein) (CLARP) (Cellular FLICE-like inhibitory protein) (c-FLIP) (FADD-like antiapoptotic molecule 1) (FLAME-1) (Inhibitor of FLICE) (I-FLICE) (MACH-related inducer of toxicity) (MRIT) (Usurpin) [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]|
|[Ifitm3] Interferon-induced transmembrane protein 3 (Dispanin subfamily A member 2b) (DSPA2b) (Fragilis protein) (Interferon-inducible protein 15) (Mouse ifitm-like protein 1) (Mil-1)|
|[Hivep3 KBP1 Kiaa1555 Krc Rc shn3 Zas3] Transcription factor HIVEP3 (Human immunodeficiency virus type I enhancer-binding protein 3 homolog) (KB-binding and recognition component) (Kappa-B and V(D)J recombination signal sequences-binding protein) (Kappa-binding protein 1) (KBP-1) (Recombinant component) (Schnurri-3) (Zinc finger protein ZAS3)|
|[Ccdc80 Urb] Coiled-coil domain-containing protein 80 (Up-regulated in BRS-3 deficient mouse)|
|[Nlrp1a Card7 Nalp1 Nalp1a Nlrp1] NACHT, LRR and PYD domains-containing protein 1a (Caspase recruitment domain-containing protein 7) (Death effector filament-forming ced-4-like apoptosis protein) (Nucleotide-binding domain and caspase recruitment domain)|
|[Nlrc4 Card12 Ipaf] NLR family CARD domain-containing protein 4 (Caspase recruitment domain-containing protein 12) (Ice protease-activating factor) (Ipaf)|
|[Dffb Cad] DNA fragmentation factor subunit beta (EC 3.-.-.-) (Caspase-activated deoxyribonuclease) (CAD) (Caspase-activated DNase) (DNA fragmentation factor 40 kDa subunit) (DFF-40)|
|[CASP3 CPP32] Caspase-3 (CASP-3) (EC 126.96.36.199) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]|
|[Espl1 Esp1 Kiaa0165] Separin (EC 188.8.131.52) (Caspase-like protein ESPL1) (Extra spindle poles-like 1 protein) (Separase)|
|[Slc39a4 Zip4] Zinc transporter ZIP4 (Activated in W/Wv mouse stomach 2) (mAWMS2) (Solute carrier family 39 member 4) (Zrt- and Irt-like protein 4) (ZIP-4)|
|[Casp3 Cpp32] Caspase-3 (CASP-3) (EC 184.108.40.206) (Apopain) (Cysteine protease CPP32) (CPP-32) (IRP) (LICE) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]|
|[Diablo Smac] Diablo homolog, mitochondrial (Direct IAP-binding protein with low pI) (Second mitochondria-derived activator of caspase) (Smac)|
|[Nod1 Card4] Nucleotide-binding oligomerization domain-containing protein 1 (Caspase recruitment domain-containing protein 4)|
|[Card11] Caspase recruitment domain-containing protein 11|
|[Nod2 Card15] Nucleotide-binding oligomerization domain-containing protein 2 (Caspase recruitment domain-containing protein 15)|
|[Card9] Caspase recruitment domain-containing protein 9|
|[Cacna1c Cach2 Cacn2 Cacnl1a1 Cchl1a1] Voltage-dependent L-type calcium channel subunit alpha-1C (Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle) (MELC-CC) (Mouse brain class C) (MBC) (Voltage-gated calcium channel subunit alpha Cav1.2)|
|[Adgrg2 Gpr64 Me6] Adhesion G-protein coupled receptor G2 (G-protein coupled receptor 64) (Mouse epididymis-specific protein 6) (Me6)|
|[Lsr Lisch7] Lipolysis-stimulated lipoprotein receptor (Lipolysis-stimulated receptor) (Liver-specific bHLH-Zip transcription factor) (Liver-specific gene on mouse chromosome 7 protein)|
|[Ifitm1] Interferon-induced transmembrane protein 1 (Dispanin subfamily A member 2a) (DSPA2a) (Fragilis protein 2) (Mouse ifitm-like protein 2) (Mil-2)|
| Development of a fluorescence-based cellular apoptosis reporter.|
| Pancreatic-derived factor impaired glucagon-like Peptide-1 production from GLUTag enterendorine L-cell line and intestines.|
| IL-4 administration exerts preventive effects via suppression of underlying inflammation and TNF-α-induced apoptosis in steroid-induced osteonecrosis.|
| Advanced glycation end-product (AGE) induces apoptosis in human retinal ARPE-19 cells via promoting mitochondrial dysfunction and activating the Fas-FasL signaling.|
| Caspase 3 cleavage of Pax7 inhibits self-renewal of satellite cells.|
| Regulation of gamma-secretase activating protein by the 5Lipoxygenase: in vitro and in vivo evidence.|
| Acute liver injury attenuation of a novel recombinant sTNFR through blocking hepatic apoptosis.|
| Activin A inhibits RANKL-mediated osteoclast formation, movement and function in murine bone marrow macrophage cultures.|
| JNK inhibition of VMAT2 contributes to rotenone-induced oxidative stress and dopamine neuron death.|
| Interplay of autophagy and apoptosis during murine cytomegalovirus infection of RPE cells.|