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Index / Biovis / Caspase_3 Colorimetric Assay Kit /Product Detail : K106-100 Caspase_3 Colorimetric Assay Kit
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#K106-100 Caspase_3 Colorimetric Assay Kit

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  Price : 384   EUR
435   USD
297   GBP
1613   Zloty
51387   JPY
2961   NOK
3173   SEK
434   CHF

Product name : Caspase_3 Colorimetric Assay Kit

Catalog number : K106-100

Quantity: 100 assays

Availability: Yes

Supplier name : Biovis

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More Details about

Kit Summary:
• Detection method- Absorbance (400 or 405 nm)
• Sample type- Cell and tissue lysates
• Species reactivity- Mammalian
• Kit size- Convenient sizes (25, 100, 200, 400 assays)
• Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features & Benefits:
• Simple one-step procedure; takes 1-2 hours
• Fast and convenient
• Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

Kit components:
• Cell Lysis buffer
• 2X Reaction Buffer
• DEVD-pNA (4 mM)
• DTT (1 M)
• Dilution Buffer

Description:
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

 

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Bibliography :
[30837399] [Cx43 silencing inhibits mechanical stress-induced apoptosis in mouse articular chondrocyte].
[30709047] The Bispidinone Derivative 3,7-Bis-[2-()-amino-3-(1-indol-3-yl)-propionyl]-1,5-diphenyl-3,7-diazabicyclo[3.3.1]nonan-9-one Dihydrochloride Induces an Apoptosis-Mediated Cytotoxic Effect on Pancreatic Cancer Cells In Vitro.
[30651831] Protective effects of astragaloside IV on IL-8-treated diaphragmatic muscle cells.
[30428879] Croton gratissimus leaf extracts inhibit cancer cell growth by inducing caspase 3/7 activation with additional anti-inflammatory and antioxidant activities.
[30136701] Mitogen-activated protein kinase phosphatase 1 protects PC12 cells from amyloid beta-induced neurotoxicity.
[30055552] Determination of Apoptotic Effect of Juglone on Human Bladder Cancer TCC-SUP and RT-4 Cells: An In Vitro Study.
[29792952] Anticancer mechanism of Sinapic acid in PC-3 and LNCaP human prostate cancer cell lines.
[29790063] In Vitro and In Vivo Evaluation of Antitumor Activity of Ligustrum robustum, A Chinese Herbal Tea.
[29579407] Modulatory effect of curcumin on ketamine-induced toxicity in rat thymocytes: Involvement of reactive oxygen species (ROS) and the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway.
[29545897] Effects of toxin A on K562/A02 cell proliferation, apoptosis and multi-drug resistance.