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Index / Biovis / Caspase_2 Colorimetric Assay Kit /Product Detail : K117-100 Caspase_2 Colorimetric Assay Kit
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#K117-100 Caspase_2 Colorimetric Assay Kit

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  Price : 438   EUR
497   USD
340   GBP
1842   Zloty
58687   JPY
3382   NOK
3624   SEK
496   CHF

Product name : Caspase_2 Colorimetric Assay Kit

Catalog number : K117-100

Quantity: 100 assays

Availability: Yes

Supplier name : Biovis

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More Details about

Kit Summary:
• Detection method- Absorbance (400 or 405 nm)
• Sample type- Cell and tissue lysates
• Species reactivity- Mammalian
• Kit size- Convenient sizes (25, 100, 200, 400 assays)
• Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features & Benefits:
• Simple one-step procedure; takes 1-2 hours
• Fast and convenient
• Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-2 activity.

Kit components:
• Cell Lysis Buffer
• 2X Reaction Buffer
• VDVAD-pNA (4 mM)
• DTT (1 M)
• Dilution Buffer

Description:
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Colorimetric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

 

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