Product name : Caspase_5, human recombinant
Catalog number : 1085-25
Quantity: 25 units
Supplier name : Biovis
Data sheet : Ask more or other datasheet now !
Caspase_5, human recombinant Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Caspase_5, human recombinant.
One unit of the recombinant caspase-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 5000 units/mg
PURITY: >95% by SDS-PAGE
The lyophilized caspase-5 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.
Caspase-5 (also know as ICErelIII, TY) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-5 exists in cells as an inactive proenzyme which is matured by proteolysis to yield large and small subunits. The active caspase-5 is a heterotetramer consisting of two large and two small subunits. To date the mechanism of caspase-5 activation and roles of caspase-5 in apoptosis are poorly understood.
The recombinant active human caspase-5 was expressed in E. coli. The active caspase-5 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-WEHD-pNA (Cat. #1102-200) or Ac-WEHD-AFC (1101-200).
APPLICATIONS AND USAGE:
Active caspase-5 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase-5 assay protocol, please refer to BioVision’s Caspase-5 Fluorometric or Colorimetric Assay Kits (Cat.#: K122 and K123).
For Research Use Only! Not to be used in humans.
Apoptosis Detection Kits & Reagents
Cell Fractionation System
Cell Proliferation & Senescence
Cell Damage & Repair
Adipocyte & Lipid Transfer
Molecular Biology & Reporter Assays
Growth Factors and Cytokines
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Kits Elisa; taq POLYMERASE
|WP2107: pathways in human cancer|
|[CASP5 ICH3] Caspase-5 (CASP-5) (EC 220.127.116.11) (ICE(rel)-III) (Protease ICH-3) (Protease TY) [Cleaved into: Caspase-5 subunit p20; Caspase-5 subunit p10]|
|[CASP8 MCH5] Caspase-8 (CASP-8) (EC 18.104.22.168) (Apoptotic cysteine protease) (Apoptotic protease Mch-5) (CAP4) (FADD-homologous ICE/ced-3-like protease) (FADD-like ICE) (FLICE) (ICE-like apoptotic protease 5) (MORT1-associated ced-3 homolog) (MACH) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]|
|[CASP3 CPP32] Caspase-3 (CASP-3) (EC 22.214.171.124) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]|
|[RNF34] E3 ubiquitin-protein ligase RNF34 (EC 126.96.36.199) (Caspase regulator CARP1) (Caspases-8 and -10-associated RING finger protein 1) (CARP-1) (FYVE-RING finger protein Momo) (Human RING finger homologous to inhibitor of apoptosis protein) (hRFI) (RING finger protein 34) (RING finger protein RIFF) (RING-type E3 ubiquitin transferase RNF34)|
|[CASP14] Caspase-14 (CASP-14) (EC 3.4.22.-) [Cleaved into: Caspase-14 subunit p17, mature form; Caspase-14 subunit p10, mature form; Caspase-14 subunit p20, intermediate form; Caspase-14 subunit p8, intermediate form]|
|[CASP2 ICH1 NEDD2] Caspase-2 (CASP-2) (EC 188.8.131.52) (Neural precursor cell expressed developmentally down-regulated protein 2) (NEDD-2) (Protease ICH-1) [Cleaved into: Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12]|
|[CASP9 MCH6] Caspase-9 (CASP-9) (EC 184.108.40.206) (Apoptotic protease Mch-6) (Apoptotic protease-activating factor 3) (APAF-3) (ICE-like apoptotic protease 6) (ICE-LAP6) [Cleaved into: Caspase-9 subunit p35; Caspase-9 subunit p10]|
|[CASP4 ICH2] Caspase-4 (CASP-4) (EC 220.127.116.11) (ICE and Ced-3 homolog 2) (ICH-2) (ICE(rel)-II) (Mih1) (Protease TX) [Cleaved into: Caspase-4 subunit 1; Caspase-4 subunit 2]|
|[CASP7 MCH3] Caspase-7 (CASP-7) (EC 18.104.22.168) (Apoptotic protease Mch-3) (CMH-1) (ICE-like apoptotic protease 3) (ICE-LAP3) [Cleaved into: Caspase-7 subunit p20; Caspase-7 subunit p11]|
|[CASP10 MCH4] Caspase-10 (CASP-10) (EC 22.214.171.124) (Apoptotic protease Mch-4) (FAS-associated death domain protein interleukin-1B-converting enzyme 2) (FLICE2) (ICE-like apoptotic protease 4) [Cleaved into: Caspase-10 subunit p23/17; Caspase-10 subunit p12]|
|[CASP1 IL1BC IL1BCE] Caspase-1 (CASP-1) (EC 126.96.36.199) (Interleukin-1 beta convertase) (IL-1BC) (Interleukin-1 beta-converting enzyme) (ICE) (IL-1 beta-converting enzyme) (p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10]|
|[CFLAR CASH CASP8AP1 CLARP MRIT] CASP8 and FADD-like apoptosis regulator (Caspase homolog) (CASH) (Caspase-eight-related protein) (Casper) (Caspase-like apoptosis regulatory protein) (CLARP) (Cellular FLICE-like inhibitory protein) (c-FLIP) (FADD-like antiapoptotic molecule 1) (FLAME-1) (Inhibitor of FLICE) (I-FLICE) (MACH-related inducer of toxicity) (MRIT) (Usurpin) [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]|
|[CASP6 MCH2] Caspase-6 (CASP-6) (EC 188.8.131.52) (Apoptotic protease Mch-2) [Cleaved into: Caspase-6 subunit p18; Caspase-6 subunit p11]|
|[CARD16 COP COP1] Caspase recruitment domain-containing protein 16 (Caspase recruitment domain-only protein 1) (CARD-only protein 1) (Caspase-1 inhibitor COP) (Pseudo interleukin-1 beta converting enzyme) (Pseudo-ICE) (Pseudo-IL1B-converting enzyme)|
|[PYCARD ASC CARD5 TMS1] Apoptosis-associated speck-like protein containing a CARD (hASC) (Caspase recruitment domain-containing protein 5) (PYD and CARD domain-containing protein) (Target of methylation-induced silencing 1)|
|[DFFB CAD DFF2 DFF40] DNA fragmentation factor subunit beta (EC 3.-.-.-) (Caspase-activated deoxyribonuclease) (CAD) (Caspase-activated DNase) (Caspase-activated nuclease) (CPAN) (DNA fragmentation factor 40 kDa subunit) (DFF-40)|
|[NLRP1 CARD7 DEFCAP KIAA0926 NAC NALP1] NACHT, LRR and PYD domains-containing protein 1 (Caspase recruitment domain-containing protein 7) (Death effector filament-forming ced-4-like apoptosis protein) (Nucleotide-binding domain and caspase recruitment domain)|
|[NLRC4 CARD12 CLAN CLAN1 IPAF UNQ6189/PRO20215] NLR family CARD domain-containing protein 4 (CARD, LRR, and NACHT-containing protein) (Clan protein) (Caspase recruitment domain-containing protein 12) (Ice protease-activating factor) (Ipaf)|
|[CRADD RAIDD] Death domain-containing protein CRADD (Caspase and RIP adapter with death domain) (RIP-associated protein with a death domain)|
|[NOD1 CARD4] Nucleotide-binding oligomerization domain-containing protein 1 (Caspase recruitment domain-containing protein 4)|
|[DIABLO SMAC] Diablo homolog, mitochondrial (Direct IAP-binding protein with low pI) (Second mitochondria-derived activator of caspase) (Smac)|
|[CARD8 KIAA0955 NDPP1] Caspase recruitment domain-containing protein 8 (Apoptotic protein NDPP1) (CARD-inhibitor of NF-kappa-B-activating ligand) (CARDINAL) (DACAR) (Tumor up-regulated CARD-containing antagonist of CASP9) (TUCAN)|
|[NPC2 HE1] NPC intracellular cholesterol transporter 2 (Epididymal secretory protein E1) (Human epididymis-specific protein 1) (He1) (Niemann-Pick disease type C2 protein)|
|[Hivep3 KBP1 Kiaa1555 Krc Rc shn3 Zas3] Transcription factor HIVEP3 (Human immunodeficiency virus type I enhancer-binding protein 3 homolog) (KB-binding and recognition component) (Kappa-B and V(D)J recombination signal sequences-binding protein) (Kappa-binding protein 1) (KBP-1) (Recombinant component) (Schnurri-3) (Zinc finger protein ZAS3)|
|[Casp4 Casp11 Caspl Ich3] Caspase-4 (CASP-4) (EC 184.108.40.206) (Caspase-11) (CASP-11) (Protease ICH-3) [Cleaved into: Caspase-4 subunit p10; Caspase-4 subunit p20]|
|[CARD11 CARMA1] Caspase recruitment domain-containing protein 11 (CARD-containing MAGUK protein 1) (Carma 1)|
|[GZMB CGL1 CSPB CTLA1 GRB] Granzyme B (EC 220.127.116.11) (C11) (CTLA-1) (Cathepsin G-like 1) (CTSGL1) (Cytotoxic T-lymphocyte proteinase 2) (Lymphocyte protease) (Fragmentin-2) (Granzyme-2) (Human lymphocyte protein) (HLP) (SECT) (T-cell serine protease 1-3E)|
|[RFFL RNF189 RNF34L] E3 ubiquitin-protein ligase rififylin (EC 18.104.22.168) (Caspase regulator CARP2) (Caspases-8 and -10-associated RING finger protein 2) (CARP-2) (FYVE-RING finger protein Sakura) (Fring) (RING finger and FYVE-like domain-containing protein 1) (RING finger protein 189) (RING finger protein 34-like) (RING-type E3 ubiquitin transferase rififylin)|
|[CARD14 CARMA2] Caspase recruitment domain-containing protein 14 (CARD-containing MAGUK protein 2) (Carma 2)|
|[CARD18 ICEBERG UNQ5804/PRO19611] Caspase recruitment domain-containing protein 18 (Caspase-1 inhibitor Iceberg)|
| Live-cell visualization of gasdermin D-driven pyroptotic cell death.|
| Effects of CASP5 gene overexpression on angiogenesis of HMEC-1 cells.|
| Identification of specific tethered inhibitors for caspase-5.|
| Characterization of periphilin, a widespread, highly insoluble nuclear protein and potential constituent of the keratinocyte cornified envelope.|
| Tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-induced apoptosis is dependent on activation of cysteine and serine proteases.|
| Targeting of the transcription factor Max during apoptosis: phosphorylation-regulated cleavage by caspase-5 at an unusual glutamic acid residue in position P1.|
| Identification of a cysteine protease closely related to interleukin-1 beta-converting enzyme.|