Product name : Caspase_3 Proform, mouse recombinant
Catalog number : 1183P-5
Quantity: 5 ìg
Supplier name : Biovis
Data sheet : Ask more or other datasheet now !
Caspase_3 Proform, mouse recombinant mus musculus murine Caspase_3 Proform, mouse recombinant detects proteins from variouse species most likely human.
Gene Symbol: CASP3
Accession #: Q8BNT4
Gene ID: 12367
Source: E. coli
Appearance: Lyophilized powder
Physical Form Description: Lyophilized powder
Molecular Weight: 32.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: Reconstitute to 0.1 µg/µl in PBS containing 15% glycerol.
Storage Temp.: -70°C
Shipping: gel pack
Background Information: Caspase-3 (also know as CPP32, Yama and apopain) is a member of the caspase family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. Proteolytic processing of this inactive proenzyme generates the 17 kD and 12 kD subunits which, when assembled as a tetramer (a pair of heterodimers), form the active caspase. Cascades of caspase activation have been shown to be key signal-transducing events in apoptosis. Procaspase-3, like other procaspases with short N-terminal prodomains, is considered to be a downstream or “effector” enzyme. Pro-caspase-3 can be cleaved and activated by caspase-8 and –10 which are themselves activated through interaction with death ligand receptor/adapter protein complexes (e.g., Fas/FADD). Procaspase-3 is also processed by caspase-9, the apical caspase that is activated by binding to the Apaf-1/cytochrome c/ATP “apoptosome”. Thus, activation of procaspase-3 stands at a point of convergence for the two major types of apoptosis signaling pathways-those linked to cell surface death receptors and those linked to mitochondrial release of cytochrome c. The recombinant Procaspase-3 (1183P-5) is produced by expression of a human cDNA in E. coli. The purified protein is highly activatable by treatment with active recombinant caspase-8 (1088-25, -100). We routinely test the activation using 10-30 ng of the procaspase-3 treated with 0.1-0.5 units of the active recombinant caspase-8 in 100 µl of Reaction Buffer (1068-20) for 30 minutes. Activation of procaspase-3 can be monitored using the Caspase-3 Fluorometric and Colorimetric Assay Kits (K105 and K106).
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.
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Kits Elisa; taq POLYMERASE
|[Casp4 Casp11 Caspl Ich3] Caspase-4 (CASP-4) (EC 18.104.22.168) (Caspase-11) (CASP-11) (Protease ICH-3) [Cleaved into: Caspase-4 subunit p10; Caspase-4 subunit p20]|
|[Casp3 Cpp32] Caspase-3 (CASP-3) (EC 22.214.171.124) (Apopain) (Cysteine protease CPP32) (CPP-32) (LICE) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]|
|[Casp2 Ich1 Nedd-2 Nedd2] Caspase-2 (CASP-2) (EC 126.96.36.199) (Neural precursor cell expressed developmentally down-regulated protein 2) (NEDD-2) (Protease ICH-1) [Cleaved into: Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12]|
|[Casp7 Lice2 Mch3] Caspase-7 (CASP-7) (EC 188.8.131.52) (Apoptotic protease Mch-3) (Cysteine protease LICE2) [Cleaved into: Caspase-7 subunit p20; Caspase-7 subunit p11]|
|[Casp6] Caspase-6 (CASP-6) (EC 184.108.40.206) (Apoptotic protease Mch-2) [Cleaved into: Caspase-6 subunit p18; Caspase-6 subunit p11]|
|[Casp1 Il1bc] Caspase-1 (CASP-1) (EC 220.127.116.11) (Interleukin-1 beta convertase) (IL-1BC) (Interleukin-1 beta-converting enzyme) (ICE) (IL-1 beta-converting enzyme) (p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10]|
|[Casp14] Caspase-14 (CASP-14) (EC 3.4.22.-) (Mini-ICE) (MICE) [Cleaved into: Caspase-14 subunit p17, mature form; Caspase-14 subunit p10, mature form; Caspase-14 subunit p20, intermediate form; Caspase-14 subunit p8, intermediate form]|
|[Casp9 Mch6] Caspase-9 (CASP-9) (EC 18.104.22.168) (Apoptotic protease Mch-6) (Apoptotic protease-activating factor 3) (APAF-3) (ICE-like apoptotic protease 6) (ICE-LAP6) [Cleaved into: Caspase-9 subunit p35; Caspase-9 subunit p10]|
|[Casp8] Caspase-8 (CASP-8) (EC 22.214.171.124) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]|
|[Casp12] Caspase-12 (CASP-12) (EC 3.4.22.-)|
|[Cflar Cash] CASP8 and FADD-like apoptosis regulator (Caspase homolog) (CASH) (Caspase-eight-related protein) (Casper) (Caspase-like apoptosis regulatory protein) (CLARP) (Cellular FLICE-like inhibitory protein) (c-FLIP) (FADD-like antiapoptotic molecule 1) (FLAME-1) (Inhibitor of FLICE) (I-FLICE) (MACH-related inducer of toxicity) (MRIT) (Usurpin) [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]|
|[Pappa] Pappalysin-1 (EC 126.96.36.199) (Insulin-like growth factor-dependent IGF-binding protein 4 protease) (IGF-dependent IGFBP-4 protease) (IGFBP-4ase) (Pregnancy-associated plasma protein A) (PAPP-A)|
|[Ifitm3] Interferon-induced transmembrane protein 3 (Dispanin subfamily A member 2b) (DSPA2b) (Fragilis protein) (Interferon-inducible protein 15) (Mouse ifitm-like protein 1) (Mil-1)|
|[Hivep3 KBP1 Kiaa1555 Krc Rc shn3 Zas3] Transcription factor HIVEP3 (Human immunodeficiency virus type I enhancer-binding protein 3 homolog) (KB-binding and recognition component) (Kappa-B and V(D)J recombination signal sequences-binding protein) (Kappa-binding protein 1) (KBP-1) (Recombinant component) (Schnurri-3) (Zinc finger protein ZAS3)|
|[Ccdc80 Urb] Coiled-coil domain-containing protein 80 (Up-regulated in BRS-3 deficient mouse)|
|[Nlrp1a Card7 Nalp1 Nalp1a Nlrp1] NACHT, LRR and PYD domains-containing protein 1a (Caspase recruitment domain-containing protein 7) (Death effector filament-forming ced-4-like apoptosis protein) (Nucleotide-binding domain and caspase recruitment domain)|
|[Nlrc4 Card12 Ipaf] NLR family CARD domain-containing protein 4 (Caspase recruitment domain-containing protein 12) (Ice protease-activating factor) (Ipaf)|
|[Dffb Cad] DNA fragmentation factor subunit beta (EC 3.-.-.-) (Caspase-activated deoxyribonuclease) (CAD) (Caspase-activated DNase) (DNA fragmentation factor 40 kDa subunit) (DFF-40)|
|[CASP3 CPP32] Caspase-3 (CASP-3) (EC 188.8.131.52) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]|
|[Espl1 Esp1 Kiaa0165] Separin (EC 184.108.40.206) (Caspase-like protein ESPL1) (Extra spindle poles-like 1 protein) (Separase)|
|[Slc39a4 Zip4] Zinc transporter ZIP4 (Activated in W/Wv mouse stomach 2) (mAWMS2) (Solute carrier family 39 member 4) (Zrt- and Irt-like protein 4) (ZIP-4)|
|[Casp3 Cpp32] Caspase-3 (CASP-3) (EC 220.127.116.11) (Apopain) (Cysteine protease CPP32) (CPP-32) (IRP) (LICE) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]|
|[Diablo Smac] Diablo homolog, mitochondrial (Direct IAP-binding protein with low pI) (Second mitochondria-derived activator of caspase) (Smac)|
|[Nod1 Card4] Nucleotide-binding oligomerization domain-containing protein 1 (Caspase recruitment domain-containing protein 4)|
|[Card11] Caspase recruitment domain-containing protein 11|
|[Nod2 Card15] Nucleotide-binding oligomerization domain-containing protein 2 (Caspase recruitment domain-containing protein 15)|
|[C3] Complement C3 (HSE-MSF) [Cleaved into: Complement C3 beta chain; C3-beta-c (C3bc); Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein (ASP) (C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2]|
|[Card9] Caspase recruitment domain-containing protein 9|
|[Cacna1c Cach2 Cacn2 Cacnl1a1 Cchl1a1] Voltage-dependent L-type calcium channel subunit alpha-1C (Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle) (MELC-CC) (Mouse brain class C) (MBC) (Voltage-gated calcium channel subunit alpha Cav1.2)|
|[Adgrg2 Gpr64 Me6] Adhesion G-protein coupled receptor G2 (G-protein coupled receptor 64) (Mouse epididymis-specific protein 6) (Me6)|
| The Drosophila inhibitor of apoptosis D-IAP1 suppresses cell death induced by the caspase drICE.|