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Index / Arbor / Cyclic AMP Direct Chemiluminescent EIA kit (One Plate) /Product Detail : K019-C1 Cyclic AMP Direct Chemiluminescent EIA kit (One Plate)
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#K019-C1 Cyclic AMP Direct Chemiluminescent EIA kit (One Plate)

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  Price : 350   EUR
398   USD
272   GBP
1474   Zloty
46949   JPY
2706   NOK
2899   SEK
396   CHF

Product name : Cyclic AMP Direct Chemiluminescent EIA kit (One Plate)

Catalog number : K019-C1

Quantity: 1x96 well plate

Availability: Yes

Supplier name : Arbor

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More Details about

 

FEATURES

  • Most Sensitive
  • Multi Species
  • USE - Measure cAMP in 2 hours
  • SAMPLE - Lysates, Urine, Plasma, Saliva, Tissue
  • SAMPLES/KIT - 38 or 230 in Duplicate
  • SENSITIVITY - Measure < 1 fmol cAMP per sample
  • STABILITY - Stable 4˚C Liquid Reagents

DESCRIPTION

The DetectX® Cyclic AMP (cAMP) Chemiluminescent Direct Immunoassay (CLIA) kits are designed to quantitatively measure cAMP present in cell lysates, plasma, urine, saliva, tissue and culture media samples. The supplied Sample Diluent will lyse cells, stabilize cAMP and stop phosphodiesterase activity. A cAMP standard is provided to generate a standard curve for the assay. The supplied Plate Primer solution is added to the wells of a coated white microtiter plate, followed by standards or diluted samples. A cAMP-peroxidase conjugate is then added to the wells.

The binding reaction is initiated by the addition of a polyclonal antibody to cAMP. After a 2 hour incubation the plate is washed and chemiluminescent substrate is added. The substrate immediately reacts with the bound cAMP-peroxidase conjugate. The generated chemiluminescent glow signal is measured. The concentration of the cAMP in the sample is calculated, after making correction for the dilution.

Cyclic AMP (cAMP) is one of the most important second messengers and a key intracellular regulator. Discovered by Sutherland and Rall in 1957, it functions as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Cyclic AMP is produced by the enzyme adenylate cyclase, and the enzyme is activated by the hormones glucagon and adrenaline and by G protein. cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterase. Other biological actions of cAMP include regulation of innate immune functioning, axon regeneration, cancer, and inflammation.

Cyclic AMP Chemiluminescent Standard Curves

 

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0043004
http://www.jbc.org/content/early/2012/09/27/jbc.M112.413609.short
 

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