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Lentiviral Dual Reporter: MSCV-GFP-T2A-Luciferase plasmid (10 ug)
/Product Detail : BLIV301PA-1 Lentiviral Dual Reporter: MSCV-GFP-T2A-Luciferase plasmid (10 ug)
Product name : Lentiviral Dual Reporter: MSCV-GFP-T2A-Luciferase plasmid (10 ug)
Catalog number : BLIV301PA-1
Quantity: 10 ug
Supplier name : SBI
Data sheet : Ask more or other datasheet now !
Lentivector-based imaging vectors
Lentivector-based imaging systems offer safe, stable and effective gene delivery in virtually all mammalian cells in tissue culture and in whole animal models. In addition, SBI offers the same imaging lentivectors used in Nature Protocols bioluminescence imaging of transplanted embryonic stem (ES) cells. Ex vivo BLI of undifferentiated double fusion (DF) human ES cells (H9 line) are shown in the left panel. The implanted ES cells were tracked for in vivo kinetics of transplanted DF human ES (hES) cells and DF hES cell-derived cardiomyocytes with BLI. Representative images from a single animal receiving intramyocardial injection of 1 x10^6 undifferentiated DF hES cells (upper right panel) or DF hES-derived cardiomyocytes (lower right panel) are shown.
Bioluminescent and Fluorescent Imaging Vector Background and Vector Formats
Lentivectors and Minicircle DNAs for Non-invasive Monitoring of Cellular Dynamics In Vivo
Molecular imaging techniques to visualize cell kinetics in small animals have resulted in an explosion in the knowledge of tumors, infectious diseases, and stem cell biology. The sensitivity and accuracy of in vitro and in vivo cell monitoring offers several advantages over traditional methods through animal sacrificing and histological analysis. Molecular imaging, for example, is normally non-invasive and allows for quantitatively assessing tumor growth and the effects of therapy over time. Over the past decade, significant advances have been made in molecular imaging technology, which include bioluminescence imaging (BLI), fluorescence imaging (FLI) and enzyme-based positron emission tomography (PET). SBI has created BLI and FLI dual reporter lentivectors and minicircle DNA constructs and D-Luciferin reagents to perform molecular imaging in vivo.
BLI uses light generated from a luciferase enzyme-substrate and an ultrasensitive cooled charge-coupled camera for signal detection. BLI may have the highest imaging sensitivity (even down to the one cell level), is high-throughput, easy-to-use system, and low cost.
FLI uses red/green fluorescent protein (RFP or GFP) as a signal. Signal generation is achieved by exciting the fluorescent proteins at a given light wavelength and detection light emission at another wavelength with a charge-coupled camera. Compared to BLI, FLI is less sensitive with higher background. In most cases, FLI is used for live imaging of shallow tissues. RFP and GFP give more histological information and can be readily used for cell sorting. FLI is typically coupled with BLI to provide an additional means for cell selection, sorting, thus gaining more histological and cellular positional information.
PET is an enzyme-based positron emission tomography techniques that uses HSV1-tk (Thymidine Kinase, TK) as a probe. Signal generation is achieved by the retention of radioisotope labeled chemicals for SPECT or PET imaging. This modality can be used for large animals or humans with detailed 3-dimensional capability but, it is more expensive to use this system. HSV1-tk, however can serve as therapeutic transgene (e.g. a suicide gene) because when cells express HSV1-tk, expression can be turned off by delivery of a pharmacological dose of the antiviral prodrugs, Ganciclovir or Penciclovir.
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