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Index / SBI / pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug /Product Detail : CD527A-1 pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug
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#CD527A-1 pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug

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  Price : 621   EUR
705   USD
482   GBP
2611   Zloty
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5136   SEK
703   CHF

Product name : pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug

Catalog number : CD527A-1

Quantity: 10 ug

Availability: Yes

Supplier name : SBI

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More Details about

HIV-based lentivectors


Description

The CD51X Dual Promoter Series
High level of expression from either the CMV, UbC or MSCV promoter. The multiple cloning site (MCS) located downstream of a promoter allows for convenient cloning of your gene of interest. The SV40 polyadenylation signal in both vectors enables efficient termination of transcription, resulting in high levels of steady-state mRNA. Downstream of your expression cassette is an EF1 promoter driving the expression of either the Puro, GFP, RFP, Neo or Hygro marker genes in the Single marker options.
Lentivector CD515B-1 MCS does not have a unique EcoRI site, as there is an additional EcoRI site in the Hygro marker gene.

SBI also offers a Dual marker cDNA expression vector that features both GFP or RFP along with Puro markers that are co-expressed (GFP-Puro, cat# CD513B-1 and CD550A-1, RFP-Puro, cat# CD516B-2). The CD550A-1 lentivector features an additional BGH poly-A site in between the EF1 promoter and the downstream PGK promoter to enhance EF1-driven expression of your cDNA clone.

The CD52X EF1 Promoter Series with T2A Co-expressed markers
SBI’s cDNA expression vectors using the EF1 promoter driving expression also incorporates the 2A-like sequence (T2A) from the insect virus Thosea asigna to mediate the co-expression of a reporter gene with the target cDNA. Reporter genes have been cloned at either the first or second positions relative to the T2A element, and both markers achieved high expression levels at either position.



The CD53X EF1 Promoter Series with IRES Co-expressed markers
SBI’s cDNA expression vectors using the EF1 promoter driving expression also incorporates the Viral IRES (Internal Ribosomal Entry Site) sequence to mediate the co-expression of a reporter gene with the target cDNA. Reporter genes have been cloned at either the first or second positions relative to the IRES element, and both markers achieved high expression levels at either position.

MSCV Promoter Series
The MSCV CpG-deficient promoter is the 5’-LTR promoter of murine stem cell virus that is incorporated into the 3’ HIV LTR of the cDNA expression lentivector. After integration into genomic DNA, the 3’MSCV/LTR will replace the 5’LTR and provide a high level of expression of the target gene and the downstream reporter gene. This promoter configuration has durable activity in most hematopoietic, embryonic and stem cells.

FIV-based lentivectors

Description

CMV promoter: promotes a high level of expression of your gene of interest in a wide variety of cell lines.
Multiple Cloning Site (MCS): for cloning the gene of interest in MCS located downstream of CMV promoter.
Hybrid CMV-5LTR promoter: provides a high level of expression of the full-length viral transcript in producer 293 cells.

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Pathways :
WP1263: Mitochondrial Gene Expression
WP1301: Mitochondrial Gene Expression
WP1368: Mitochondrial Gene Expression
WP1821: Gene Expression
WP1963: The effect of Glucocorticoids on target gene expression
WP391: Mitochondrial Gene Expression
WP928: Mitochondrial Gene Expression

Related Genes :
[TEF1 YPR080W P9513.7; TEF2 YBR118W YBR0913] Elongation factor 1-alpha (EF-1-alpha) (Eukaryotic elongation factor 1A) (eEF1A) (Translation elongation factor 1A)
[EFB1 TEF5 YAL003W] Elongation factor 1-beta (EF-1-beta) (Eukaryotic elongation factor 1Balpha) (eEF1Balpha) (Translation elongation factor 1B alpha)
[CAM1 TEF3 YPL048W] Elongation factor 1-gamma 1 (EF-1-gamma 1) (Calcium and membrane-binding protein 1) (Calcium phospholipid-binding protein) (CPBP) (Eukaryotic elongation factor 1Bgamma 1) (eEF1Bgamma 1) (Translation elongation factor 1B gamma 1)
[dusA SP60_00530] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[pyrG SP60_06995] CTP synthase (EC 6.3.4.2) (Cytidine 5'-triphosphate synthase) (Cytidine triphosphate synthetase) (CTP synthetase) (CTPS) (UTP--ammonia ligase)
[TEF4 EFC1 YKL081W] Elongation factor 1-gamma 2 (EF-1-gamma 2) (Eukaryotic elongation factor 1Bgamma 2) (eEF1Bgamma 2) (Translation elongation factor 1B gamma 2)
[dapB SP60_01615] 4-hydroxy-tetrahydrodipicolinate reductase (HTPA reductase) (EC 1.17.1.8)
[coaX SP60_01295] Type III pantothenate kinase (EC 2.7.1.33) (PanK-III) (Pantothenic acid kinase)
[iscS SP60_07650] Cysteine desulfurase IscS (EC 2.8.1.7)
[mobA SP60_06820] Molybdenum cofactor guanylyltransferase (MoCo guanylyltransferase) (EC 2.7.7.77) (GTP:molybdopterin guanylyltransferase) (Mo-MPT guanylyltransferase) (Molybdopterin guanylyltransferase) (Molybdopterin-guanine dinucleotide synthase) (MGD synthase)
[leuB SP60_04660] 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (3-IPM-DH) (Beta-IPM dehydrogenase) (IMDH)
[atp6 SPMIT.07] ATP synthase subunit a (F-ATPase protein 6)
[mutM fpg SP60_01330] Formamidopyrimidine-DNA glycosylase (Fapy-DNA glycosylase) (EC 3.2.2.23) (DNA-(apurinic or apyrimidinic site) lyase MutM) (AP lyase MutM) (EC 4.2.99.18)
[pdxH SP60_00570] Pyridoxine/pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5) (PNP/PMP oxidase) (PNPOx) (Pyridoxal 5'-phosphate synthase)
[aroB SP60_01275] 3-dehydroquinate synthase (DHQS) (EC 4.2.3.4)
[ribB SP60_00140] 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase) (EC 4.1.99.12)
[lon SP60_05455] Lon protease (EC 3.4.21.53) (ATP-dependent protease La)
[ileS SP60_01280] Isoleucine--tRNA ligase (EC 6.1.1.5) (Isoleucyl-tRNA synthetase) (IleRS)
[aceK SP60_01900] Isocitrate dehydrogenase kinase/phosphatase (IDH kinase/phosphatase) (IDHK/P) (EC 2.7.11.5) (EC 3.1.3.-)
[plsY SP60_03085] Glycerol-3-phosphate acyltransferase (Acyl-PO4 G3P acyltransferase) (Acyl-phosphate--glycerol-3-phosphate acyltransferase) (G3P acyltransferase) (GPAT) (EC 2.3.1.275) (Lysophosphatidic acid synthase) (LPA synthase)
[ddl SP60_06390] D-alanine--D-alanine ligase (EC 6.3.2.4) (D-Ala-D-Ala ligase) (D-alanylalanine synthetase)
[sucC SP60_05120] Succinate--CoA ligase [ADP-forming] subunit beta (EC 6.2.1.5) (Succinyl-CoA synthetase subunit beta) (SCS-beta)
[pepA SP60_05470] Probable cytosol aminopeptidase (EC 3.4.11.1) (Leucine aminopeptidase) (LAP) (EC 3.4.11.10) (Leucyl aminopeptidase)
[accD SP60_03635] Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (ACCase subunit beta) (Acetyl-CoA carboxylase carboxyltransferase subunit beta) (EC 2.1.3.15)
[nnrE nnrD DDP35_06675] Multifunctional fusion protein [Includes: ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC 4.2.1.136) (ADP-dependent NAD(P)HX dehydratase); NAD(P)H-hydrate epimerase (EC 5.1.99.6) (NAD(P)HX epimerase)]
[carB SP60_01920] Carbamoyl-phosphate synthase large chain (EC 6.3.5.5) (Carbamoyl-phosphate synthetase ammonia chain)
[carA SP60_01930] Carbamoyl-phosphate synthase small chain (EC 6.3.5.5) (Carbamoyl-phosphate synthetase glutamine chain)
[fabH SP60_00260] 3-oxoacyl-[acyl-carrier-protein] synthase 3 (EC 2.3.1.180) (3-oxoacyl-[acyl-carrier-protein] synthase III) (Beta-ketoacyl-ACP synthase III) (KAS III)
[tef-1 B15B3.060 NCU02003] Elongation factor 1-alpha (EF-1-alpha)
[thiG SP60_01655] Thiazole synthase (EC 2.8.1.10)

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