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Index / Biovis / Caspase-1, mouse recombinant CASP1 Mouse /Product Detail : 1181-100 Caspase-1, mouse recombinant CASP1 Mouse
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#1181-100 Caspase-1, mouse recombinant CASP1 Mouse

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  Price : 384   EUR
435   USD
297   GBP
1613   Zloty
51387   JPY
2961   NOK
3173   SEK
434   CHF

Product name : Caspase-1, mouse recombinant CASP1 Mouse

Catalog number : 1181-100

Quantity: 100 units

Availability: Yes

Supplier name : Biovis

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About this Product :

Caspase-1, mouse recombinant CASP1 Mouse recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. Please read carefully the data sheet of the Caspase-1, mouse recombinant CASP1 Mouse.
http://mybiofast.com/ver.php?search=rec.&submit=Search

Caspase-1, mouse recombinant CASP1 Mouse mus musculus murine Caspase-1, mouse recombinant CASP1 Mouse detects proteins from variouse species most likely human.
http://mybiofast.com/ver.php?search=musculus&submit=Search

More Details about

UNIT DEFINATION:
One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate Ac-YVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.


FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 20000 units/mg


STORAGE CONDITIONS:
The lyophilized caspase-1 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.


DESCRIPTION:
Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form.
The recombinant active mouse caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200).


APPLICATIONS AND USAGE:
Active caspase-1 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase-1 assay protocol, please refer to BioVision’s Caspase-1 Fluorometric or Colorimetric Assay Kits (Cat.#: K110 and K111).

 

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Growth Factors and Cytokines
Monoclonal and Polyclonal Antibodies

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Pathways :
WP1337: MAPK signaling pathway
WP1351: Apoptosis
WP100: Glutathione metabolism
WP1016: Wnt Signaling Pathway
WP1018: Apoptosis
WP1039: Glutathione metabolism
WP104: Alanine and aspartate metabolism
WP1056: Complement and Coagulation Cascades
WP106: Alanine and aspartate metabolism
WP1114: MAPK signaling pathway
WP1126: Tryptophan metabolism
WP1135: Wnt Signaling Pathway
WP1137: Apoptosis
WP1172: Complement and Coagulation Cascades
WP1254: Apoptosis
WP1290: Apoptosis
WP1313: Insulin Signaling
WP1315: IL-5 Signaling Pathway
WP1318: Eicosanoid Synthesis
WP1321: Myometrial Relaxation and Contraction Pathways
WP1323: EGFR1 Signaling Pathway
WP1330: estrogen signalling
WP1331: Adipogenesis
WP1333: Hypothetical Network for Drug Addiction
WP1338: Peptide GPCRs

Related Genes :
[Casp1 Il1bc] Caspase-1 (CASP-1) (EC 3.4.22.36) (Interleukin-1 beta convertase) (IL-1BC) (Interleukin-1 beta-converting enzyme) (ICE) (IL-1 beta-converting enzyme) (p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10]
[CASP1 IL1BC IL1BCE] Caspase-1 (CASP-1) (EC 3.4.22.36) (Interleukin-1 beta convertase) (IL-1BC) (Interleukin-1 beta-converting enzyme) (ICE) (IL-1 beta-converting enzyme) (p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10]
[Casp1 Il1bc Il1bce] Caspase-1 (CASP-1) (EC 3.4.22.36) (Interleukin-1 beta convertase) (IL-1BC) (Interleukin-1 beta-converting enzyme) (ICE) (IL-1 beta-converting enzyme) (p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10]
[CASP1 At2g36100 F9C22.3] Casparian strip membrane protein 1 (AtCASP1)
[CASP1 IL1BC] Caspase-1 (CASP-1) (EC 3.4.22.36) (Interleukin-1 beta convertase) (IL-1BC) (Interleukin-1 beta-converting enzyme) (ICE) (IL-1 beta-converting enzyme) (p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10]
[CASP1 IL1BC] Caspase-1 (CASP-1) (EC 3.4.22.36) (Interleukin-1 beta convertase) (IL-1BC) (Interleukin-1 beta-converting enzyme) (ICE) (IL-1 beta-converting enzyme) (p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10]
[CASP1 IL1BC] Caspase-1 (CASP-1) (EC 3.4.22.36) (Interleukin-1 beta convertase) (IL-1BC) (Interleukin-1 beta-converting enzyme) (ICE) (IL-1 beta-converting enzyme) (p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10]
[CASP1 IL1BC] Caspase-1 (CASP-1) (EC 3.4.22.36) (Interleukin-1 beta convertase) (IL-1BC) (Interleukin-1 beta-converting enzyme) (ICE) (IL-1 beta-converting enzyme) (p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10]
[Nlrp1a Card7 Nalp1 Nalp1a Nlrp1] NACHT, LRR and PYD domains-containing protein 1a (Caspase recruitment domain-containing protein 7) (Death effector filament-forming ced-4-like apoptosis protein) (Nucleotide-binding domain and caspase recruitment domain)
[Casp4 Casp11 Caspl Ich3] Caspase-4 (CASP-4) (EC 3.4.22.64) (Caspase-11) (CASP-11) (Protease ICH-3) [Cleaved into: Caspase-4 subunit p10; Caspase-4 subunit p20]
[Nlrp1b Nalp1b] NACHT, LRR and PYD domains-containing protein 1b allele 2
[Nlrp1b Nalp1b] NACHT, LRR and PYD domains-containing protein 1b allele 1
[Nlrp3 Cias1 Mmig1 Nalp3 Pypaf1] NACHT, LRR and PYD domains-containing protein 3 (Cold autoinflammatory syndrome 1 protein homolog) (Cryopyrin) (Mast cell maturation-associated-inducible protein 1) (PYRIN-containing APAF1-like protein 1)
[Nod2 Card15] Nucleotide-binding oligomerization domain-containing protein 2 (Caspase recruitment domain-containing protein 15)
[Nlrc4 Card12 Ipaf] NLR family CARD domain-containing protein 4 (Caspase recruitment domain-containing protein 12) (Ice protease-activating factor) (Ipaf)
[Nlrp1b Nalp1b] NACHT, LRR and PYD domains-containing protein 1b allele 5
[Gsdmdc1 Gsdmd] Gasdermin-D (Gasdermin domain-containing protein 1) [Cleaved into: Gasdermin-D, N-terminal (GSDMD-NT); Gasdermin-D, C-terminal (GSDMD-CT)]
[Pycard Asc] Apoptosis-associated speck-like protein containing a CARD (mASC) (PYD and CARD domain-containing protein)
[Nlrp1b Nalp1b] NACHT, LRR and PYD domains-containing protein 1b allele 4
[Nlrp1b Nalp1b] NACHT, LRR and PYD domains-containing protein 1b allele 3
[Nlrp6 Nalp6 Navr Pypaf5] NACHT, LRR and PYD domains-containing protein 6 (Angiotensin II/vasopressin receptor) (Non-angiotensin-vasopressin receptor) (Non-AVR) (PYRIN-containing APAF1-like protein 5-like)
[Cgas Mb21d1] Cyclic GMP-AMP synthase (cGAMP synthase) (cGAS) (m-cGAS) (EC 2.7.7.86) (2'3'-cGAMP synthase) (Mab-21 domain-containing protein 1)
[Il1b] Interleukin-1 beta (IL-1 beta)
[Nlrp10 Nalp10 Pynod] NACHT, LRR and PYD domains-containing protein 10
[NLRP1 CARD7 DEFCAP KIAA0926 NAC NALP1] NACHT, LRR and PYD domains-containing protein 1 (Caspase recruitment domain-containing protein 7) (Death effector filament-forming ced-4-like apoptosis protein) (Nucleotide-binding domain and caspase recruitment domain)
[Hmgb1 Hmg-1 Hmg1] High mobility group protein B1 (High mobility group protein 1) (HMG-1)
[CASP1 CR201_G0025526] CASP1 isoform 4 (CASP1 isoform 5) (Caspase 1)
[Nlrp9b Nalp9 Nalp9b] NACHT, LRR and PYD domains-containing protein 9B (NALP-delta)
[CARD16 COP COP1] Caspase recruitment domain-containing protein 16 (Caspase recruitment domain-only protein 1) (CARD-only protein 1) (Caspase-1 inhibitor COP) (Pseudo interleukin-1 beta converting enzyme) (Pseudo-ICE) (Pseudo-IL1B-converting enzyme)
[Naip5 Birc1e Naip-rs3] Baculoviral IAP repeat-containing protein 1e (Neuronal apoptosis inhibitory protein 5)

Bibliography :
[29414788] Extensive peptide and natural protein substrate screens reveal that mouse caspase-11 has much narrower substrate specificity than caspase-1.
[29393276] Isoliquiritigenin Ameliorates Indomethacin-Induced Small Intestinal Damage by Inhibiting NOD-Like Receptor Family, Pyrin Domain-Containing 3 Inflammasome Activation.