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Index / Biovis / Caspase-10_a, human recombinant CASP10 Human /Product Detail : 1090A-25 Caspase-10_a, human recombinant CASP10 Human
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  Price : 241   EUR
274   USD
187   GBP
1015   Zloty
32349   JPY
1864   NOK
1997   SEK
273   CHF

Product name : Caspase-10_a, human recombinant CASP10 Human

Catalog number : 1090A-25

Quantity: 25 units

Availability: Yes

Supplier name : Biovis

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About this Product :

Caspase-10_a, human recombinant CASP10 Human recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. Please read carefully the data sheet of the Caspase-10_a, human recombinant CASP10 Human.
http://mybiofast.com/ver.php?search=rec.&submit=Search

Caspase-10_a, human recombinant CASP10 Human Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Caspase-10_a, human recombinant CASP10 Human.
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More Details about

UNIT DEFINITION:
One unit of the recombinant caspase-10/a is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.


FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 8000 units/mg
PURITY: >90% by SDS-PAGE


STORAGE CONDITIONS:
The lyophilized caspase-10 is stable for 1 year at –70°C. Following reconstitution in PBS+15% glycerol, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.


DESCRIPTION:
Caspase-10/a (also known as Mch4) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-10 also exists in cells as an inactive proenzyme. During apoptosis procaspase-10 is processed at aspartate residues to form active caspase-10 which consists of two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form.
The recombinant active human caspase-10 was expressed in E. coli. The active caspase-10 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).


APPLICATIONS AND USAGE:
Active caspase10/a is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase assay protocol, please refer to BioVision’s Caspase Fluorometric or Colorimetric Assay Kits.


For Research Use Only! Not to be used in humans.

 

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Pathways :
WP1772: Apoptosis Modulation and Signaling
WP2107: pathways in human cancer
WP1018: Apoptosis
WP1019: FAS pathway and Stress induction of HSP regulation
WP1047: TNF-alpha NF-kB Signaling Pathway
WP1137: Apoptosis
WP1138: FAS pathway and Stress induction of HSP regulation
WP1163: TNF-alpha NF-kB Signaling Pathway
WP1254: Apoptosis
WP1290: Apoptosis
WP1965: VEGF-receptor Signal Transduction
WP2256: Integrated Pancreatic Cancer Pathway
WP2359: Parkin-Ubiquitin Proteasomal System pathway
WP2377: Integrated Pancreatic Cancer Pathway
WP2428: the quecetin and apoptosis pathway
WP246: TNF-alpha NF-kB Signaling Pathway
WP254: Apoptosis
WP314: FAS pathway and Stress induction of HSP regulation
WP457: TNF-alpha NF-kB Signaling Pathway
WP571: FAS pathway and Stress induction of HSP regulation
WP787: Apoptosis
WP788: FAS pathway and Stress induction of HSP regulation
WP811: TNF-alpha NF-kB Signaling Pathway
WP88: Toll Like Receptor signaling
WP89: FAS pathway and Stress induction of HSP regulation

Related Genes :
[CASP10 MCH4] Caspase-10 (CASP-10) (EC 3.4.22.63) (Apoptotic protease Mch-4) (FAS-associated death domain protein interleukin-1B-converting enzyme 2) (FLICE2) (ICE-like apoptotic protease 4) [Cleaved into: Caspase-10 subunit p23/17; Caspase-10 subunit p12]
[RNF34] E3 ubiquitin-protein ligase RNF34 (EC 2.3.2.27) (Caspase regulator CARP1) (Caspases-8 and -10-associated RING finger protein 1) (CARP-1) (FYVE-RING finger protein Momo) (Human RING finger homologous to inhibitor of apoptosis protein) (hRFI) (RING finger protein 34) (RING finger protein RIFF) (RING-type E3 ubiquitin transferase RNF34)
[PPIG] Peptidyl-prolyl cis-trans isomerase G (PPIase G) (Peptidyl-prolyl isomerase G) (EC 5.2.1.8) (CASP10) (Clk-associating RS-cyclophilin) (CARS-Cyp) (CARS-cyclophilin) (SR-cyclophilin) (SR-cyp) (SRcyp) (Cyclophilin G) (Rotamase G)
[CASP8 MCH5] Caspase-8 (CASP-8) (EC 3.4.22.61) (Apoptotic cysteine protease) (Apoptotic protease Mch-5) (CAP4) (FADD-homologous ICE/ced-3-like protease) (FADD-like ICE) (FLICE) (ICE-like apoptotic protease 5) (MORT1-associated ced-3 homolog) (MACH) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]
[RFFL RNF189 RNF34L] E3 ubiquitin-protein ligase rififylin (EC 2.3.2.27) (Caspase regulator CARP2) (Caspases-8 and -10-associated RING finger protein 2) (CARP-2) (FYVE-RING finger protein Sakura) (Fring) (RING finger and FYVE-like domain-containing protein 1) (RING finger protein 189) (RING finger protein 34-like) (RING-type E3 ubiquitin transferase rififylin)
[CFLAR CASH CASP8AP1 CLARP MRIT] CASP8 and FADD-like apoptosis regulator (Caspase homolog) (CASH) (Caspase-eight-related protein) (Casper) (Caspase-like apoptosis regulatory protein) (CLARP) (Cellular FLICE-like inhibitory protein) (c-FLIP) (FADD-like antiapoptotic molecule 1) (FLAME-1) (Inhibitor of FLICE) (I-FLICE) (MACH-related inducer of toxicity) (MRIT) (Usurpin) [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]
[RIOK3 SUDD] Serine/threonine-protein kinase RIO3 (EC 2.7.11.1) (RIO kinase 3) (sudD homolog)
[XIAP API3 BIRC4 IAP3] E3 ubiquitin-protein ligase XIAP (EC 2.3.2.27) (Baculoviral IAP repeat-containing protein 4) (IAP-like protein) (ILP) (hILP) (Inhibitor of apoptosis protein 3) (IAP-3) (hIAP-3) (hIAP3) (RING-type E3 ubiquitin transferase XIAP) (X-linked inhibitor of apoptosis protein) (X-linked IAP)
[Casp8] Caspase-8 (CASP-8) (EC 3.4.22.61) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]
[Casp8] Caspase-8 (CASP-8) (EC 3.4.22.61) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]
[DEDD2 FLAME3 PSEC0004] DNA-binding death effector domain-containing protein 2 (DED-containing protein FLAME-3) (FADD-like anti-apoptotic molecule 3)
[RYBP DEDAF YEAF1] RING1 and YY1-binding protein (Apoptin-associating protein 1) (APAP-1) (Death effector domain-associated factor) (DED-associated factor) (YY1 and E4TF1-associated factor 1)
[RIPK1 RIP RIP1] Receptor-interacting serine/threonine-protein kinase 1 (EC 2.7.11.1) (Cell death protein RIP) (Receptor-interacting protein 1) (RIP-1)
[IFT57 DERP8 ESRRBL1 HIPPI] Intraflagellar transport protein 57 homolog (Dermal papilla-derived protein 8) (Estrogen-related receptor beta-like protein 1) (HIP1-interacting protein) (MHS4R2)
[DEDD DEDPRO1 DEFT KE05] Death effector domain-containing protein (DEDPro1) (Death effector domain-containing testicular molecule) (FLDED-1)
[CASP14] Caspase-14 (CASP-14) (EC 3.4.22.-) [Cleaved into: Caspase-14 subunit p17, mature form; Caspase-14 subunit p10, mature form; Caspase-14 subunit p20, intermediate form; Caspase-14 subunit p8, intermediate form]
[Rffl] E3 ubiquitin-protein ligase rififylin (EC 2.3.2.27) (RING finger and FYVE-like domain-containing protein 1) (Fring) (RING-type E3 ubiquitin transferase rififylin)
[Rffl] E3 ubiquitin-protein ligase rififylin (EC 2.3.2.27) (FYVE-RING finger protein Sakura) (RING finger and FYVE-like domain-containing protein 1) (RING-type E3 ubiquitin transferase rififylin)
[CASP9 MCH6] Caspase-9 (CASP-9) (EC 3.4.22.62) (Apoptotic protease Mch-6) (Apoptotic protease-activating factor 3) (APAF-3) (ICE-like apoptotic protease 6) (ICE-LAP6) [Cleaved into: Caspase-9 subunit p35; Caspase-9 subunit p10]
[CASP1 IL1BC IL1BCE] Caspase-1 (CASP-1) (EC 3.4.22.36) (Interleukin-1 beta convertase) (IL-1BC) (Interleukin-1 beta-converting enzyme) (ICE) (IL-1 beta-converting enzyme) (p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10]
[CASP5 ICH3] Caspase-5 (CASP-5) (EC 3.4.22.58) (ICE(rel)-III) (Protease ICH-3) (Protease TY) [Cleaved into: Caspase-5 subunit p20; Caspase-5 subunit p10]
[CASP3 CPP32] Caspase-3 (CASP-3) (EC 3.4.22.56) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]
[EPHA3 ETK ETK1 HEK TYRO4] Ephrin type-A receptor 3 (EC 2.7.10.1) (EPH-like kinase 4) (EK4) (hEK4) (HEK) (Human embryo kinase) (Tyrosine-protein kinase TYRO4) (Tyrosine-protein kinase receptor ETK1) (Eph-like tyrosine kinase 1)
[MAEA EMP HLC10 PIG5] E3 ubiquitin-protein transferase MAEA (EC 2.3.2.27) (Cell proliferation-inducing gene 5 protein) (Erythroblast macrophage protein) (Human lung cancer oncogene 10 protein) (HLC-10) (Macrophage erythroblast attacher) (P44EMLP)
[NPC2 HE1] NPC intracellular cholesterol transporter 2 (Epididymal secretory protein E1) (Human epididymis-specific protein 1) (He1) (Niemann-Pick disease type C2 protein)
[Rnf34] E3 ubiquitin-protein ligase RNF34 (EC 2.3.2.27) (Phafin-1) (RING finger protein 34) (RING-type E3 ubiquitin transferase RNF34)
[Rnf34] E3 ubiquitin-protein ligase RNF34 (EC 2.3.2.27) (RING finger protein 34) (RING finger protein MOMO) (RING-type E3 ubiquitin transferase RNF34)
[CCNB1IP1 C14orf18 HEI10] E3 ubiquitin-protein ligase CCNB1IP1 (EC 2.3.2.27) (Cyclin-B1-interacting protein 1) (Human enhancer of invasion 10) (RING-type E3 ubiquitin transferase CCNB1IP1)
[RNF34] E3 ubiquitin-protein ligase RNF34 (EC 2.3.2.27) (RING finger protein 34) (RING-type E3 ubiquitin transferase RNF34)
[GZMB CGL1 CSPB CTLA1 GRB] Granzyme B (EC 3.4.21.79) (C11) (CTLA-1) (Cathepsin G-like 1) (CTSGL1) (Cytotoxic T-lymphocyte proteinase 2) (Lymphocyte protease) (Fragmentin-2) (Granzyme-2) (Human lymphocyte protein) (HLP) (SECT) (T-cell serine protease 1-3E)

Bibliography :
[22926077] Trauma patients' elevated tumor necrosis related apoptosis inducing ligand (TRAIL) contributes to increased T cell apoptosis.
[22528489] Cathepsin D primes caspase-8 activation by multiple intra-chain proteolysis.
[22426257] The role of BCL11B in regulating the proliferation of human naive T cells.
[17479112] Ectopic expression of the serine protease inhibitor PI9 modulates death receptor-mediated apoptosis.
[15772077] Caspase-10 triggers Bid cleavage and caspase cascade activation in FasL-induced apoptosis.
[12527898] DEDD and DEDD2 associate with caspase-8/10 and signal cell death.
[12407100] Bile acids stimulate cFLIP phosphorylation enhancing TRAIL-mediated apoptosis.
[12010812] Inactivating mutations of CASP10 gene in non-Hodgkin lymphomas.
[11262188] Comprehensive studies on subcellular localizations and cell death-inducing activities of eight GFP-tagged apoptosis-related caspases.
[11002417] Activation of the NF-kappaB pathway by caspase 8 and its homologs.