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Index / Biovis / Caspase-2, human recombinant CASP2 Human /Product Detail : 1082-25 Caspase-2, human recombinant CASP2 Human
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#1082-25 Caspase-2, human recombinant CASP2 Human

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  Price : 241   EUR
274   USD
187   GBP
1015   Zloty
32349   JPY
1864   NOK
1997   SEK
273   CHF

Product name : Caspase-2, human recombinant CASP2 Human

Catalog number : 1082-25

Quantity: 25 units

Availability: Yes

Supplier name : Biovis

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About this Product :

Caspase-2, human recombinant CASP2 Human recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. Please read carefully the data sheet of the Caspase-2, human recombinant CASP2 Human.
http://mybiofast.com/ver.php?search=rec.&submit=Search

Caspase-2, human recombinant CASP2 Human Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Caspase-2, human recombinant CASP2 Human.
http://mybiofast.com/ver.php?search=human+&submit=Search

More Details about

UNIT DEFINATION:
One unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.


FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
PURITY: >95% by SDS-PAGE


STORAGE CONDITIONS:
The lyophilized caspase-2 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.


DESCRIPTION:
Caspase-2 (also know as Ich-1, Nedd-2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis pro-caspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed active caspase-2 is a heterotetramer consisting of two large (19 kDa) and two small (12 kDa) subunits.
The recombinant active human caspase-2 was expressed in E. coli. The active caspase-2 preferentially cleaves caspase-2 substrates (e.g., VDVAD-AFC or VDVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-VDVAD-pNA (Cat. #1072-200) or Ac-VDVAD-AFC (1071-200).


APPLICATIONS AND USAGE:
Active caspase-2 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase-2 assay protocol, please refer to BioVision’s Caspase-2 Fluorometric or Colorimetric Assay Kits (Cat.#: K116 and K117).

 

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Pathways :
WP1772: Apoptosis Modulation and Signaling
WP2107: pathways in human cancer
WP246: TNF-alpha NF-kB Signaling Pathway
WP100: Glutathione metabolism
WP1018: Apoptosis
WP1039: Glutathione metabolism
WP104: Alanine and aspartate metabolism
WP1047: TNF-alpha NF-kB Signaling Pathway
WP106: Alanine and aspartate metabolism
WP1082: Apoptosis Modulation by HSP70
WP1114: MAPK signaling pathway
WP1126: Tryptophan metabolism
WP1137: Apoptosis
WP1163: TNF-alpha NF-kB Signaling Pathway
WP1199: Apoptosis Modulation by HSP70
WP1254: Apoptosis
WP1290: Apoptosis
WP1313: Insulin Signaling
WP1321: Myometrial Relaxation and Contraction Pathways
WP1323: EGFR1 Signaling Pathway
WP1329: Alpha6-Beta4 Integrin Signaling Pathway
WP1333: Hypothetical Network for Drug Addiction
WP1337: MAPK signaling pathway
WP1344: Wnt Signaling Pathway and Pluripotency
WP1345: T Cell Receptor Signaling Pathway

Related Genes :
[CASP2 ICH1 NEDD2] Caspase-2 (CASP-2) (EC 3.4.22.55) (Neural precursor cell expressed developmentally down-regulated protein 2) (NEDD-2) (Protease ICH-1) [Cleaved into: Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12]
[Casp2 Ich1 Nedd-2 Nedd2] Caspase-2 (CASP-2) (EC 3.4.22.55) (Neural precursor cell expressed developmentally down-regulated protein 2) (NEDD-2) (Protease ICH-1) [Cleaved into: Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12]
[Casp2 Ich1] Caspase-2 (CASP-2) (EC 3.4.22.55) (Protease ICH-1) [Cleaved into: Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12]
[CASP2 ICH1] Caspase-2 (CASP-2) (EC 3.4.22.55) (ICH-1L/1S) (Protease ICH-1) [Cleaved into: Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12]
[CRADD RAIDD] Death domain-containing protein CRADD (Caspase and RIP adapter with death domain) (RIP-associated protein with a death domain)
[PIDD1 LRDD PIDD] p53-induced death domain-containing protein 1 (EC 3.4.21.-) (Leucine-rich repeat and death domain-containing protein) [Cleaved into: PIDD-N; PIDD-C; PIDD-CC]
[MZB1 MEDA7 PACAP HSPC190] Marginal zone B- and B1-cell-specific protein (Mesenteric estrogen-dependent adipose 7) (MEDA-7) (Plasma cell-induced resident endoplasmic reticulum protein) (Plasma cell-induced resident ER protein) (pERp1) (Proapoptotic caspase adapter protein)
[NOL3 ARC NOP] Nucleolar protein 3 (Apoptosis repressor with CARD) (Muscle-enriched cytoplasmic protein) (Myp) (Nucleolar protein of 30 kDa) (Nop30)
[CASP2 At3g11550 F24K9.22] Casparian strip membrane protein 2 (AtCASP2)
[RBM5 H37 LUCA15] RNA-binding protein 5 (Protein G15) (Putative tumor suppressor LUCA15) (RNA-binding motif protein 5) (Renal carcinoma antigen NY-REN-9)
[CSNK2A2 CK2A2] Casein kinase II subunit alpha' (CK II alpha') (EC 2.7.11.1)
[CSNK2A1 CK2A1] Casein kinase II subunit alpha (CK II alpha) (EC 2.7.11.1)
[RNF34] E3 ubiquitin-protein ligase RNF34 (EC 2.3.2.27) (Caspase regulator CARP1) (Caspases-8 and -10-associated RING finger protein 1) (CARP-1) (FYVE-RING finger protein Momo) (Human RING finger homologous to inhibitor of apoptosis protein) (hRFI) (RING finger protein 34) (RING finger protein RIFF) (RING-type E3 ubiquitin transferase RNF34)
[Nol3 Arc] Nucleolar protein 3 (Apoptosis repressor with CARD)
[Csnk2a1 Ckiia] Casein kinase II subunit alpha (CK II alpha) (EC 2.7.11.1)
[Pidd1 Lrdd Pidd] p53-induced death domain-containing protein 1 (EC 3.4.21.-) (Leucine-rich repeat and death domain-containing protein) [Cleaved into: PIDD-N; PIDD-C; PIDD-CC]
[Csnk2a1] Casein kinase II subunit alpha (CK II alpha) (EC 2.7.11.1)
[NPC2 HE1] NPC intracellular cholesterol transporter 2 (Epididymal secretory protein E1) (Human epididymis-specific protein 1) (He1) (Niemann-Pick disease type C2 protein)
[Cradd Raidd] Death domain-containing protein CRADD (Caspase and RIP adapter with death domain) (RIP-associated protein with a death domain)
[GZMB CGL1 CSPB CTLA1 GRB] Granzyme B (EC 3.4.21.79) (C11) (CTLA-1) (Cathepsin G-like 1) (CTSGL1) (Cytotoxic T-lymphocyte proteinase 2) (Lymphocyte protease) (Fragmentin-2) (Granzyme-2) (Human lymphocyte protein) (HLP) (SECT) (T-cell serine protease 1-3E)
[Nol3 Arc] Nucleolar protein 3 (Apoptosis repressor with CARD)
[COI] Cytochrome c oxidase subunit 1 (EC 1.9.3.1) (Fragment)
[CASP3 CPP32] Caspase-3 (CASP-3) (EC 3.4.22.56) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]
[CASP4 ICH2] Caspase-4 (CASP-4) (EC 3.4.22.57) (ICE and Ced-3 homolog 2) (ICH-2) (ICE(rel)-II) (Mih1) (Protease TX) [Cleaved into: Caspase-4 subunit 1; Caspase-4 subunit 2]
[CASP10 MCH4] Caspase-10 (CASP-10) (EC 3.4.22.63) (Apoptotic protease Mch-4) (FAS-associated death domain protein interleukin-1B-converting enzyme 2) (FLICE2) (ICE-like apoptotic protease 4) [Cleaved into: Caspase-10 subunit p23/17; Caspase-10 subunit p12]
[CASP6 MCH2] Caspase-6 (CASP-6) (EC 3.4.22.59) (Apoptotic protease Mch-2) [Cleaved into: Caspase-6 subunit p18; Caspase-6 subunit p11]
[CASP14] Caspase-14 (CASP-14) (EC 3.4.22.-) [Cleaved into: Caspase-14 subunit p17, mature form; Caspase-14 subunit p10, mature form; Caspase-14 subunit p20, intermediate form; Caspase-14 subunit p8, intermediate form]
[DNAJA1 DNAJ2 HDJ2 HSJ2 HSPF4] DnaJ homolog subfamily A member 1 (DnaJ protein homolog 2) (HSDJ) (Heat shock 40 kDa protein 4) (Heat shock protein J2) (HSJ-2) (Human DnaJ protein 2) (hDj-2)
[Csnk2a2] Casein kinase II subunit alpha' (CK II alpha') (EC 2.7.11.1)
[CASP8 MCH5] Caspase-8 (CASP-8) (EC 3.4.22.61) (Apoptotic cysteine protease) (Apoptotic protease Mch-5) (CAP4) (FADD-homologous ICE/ced-3-like protease) (FADD-like ICE) (FLICE) (ICE-like apoptotic protease 5) (MORT1-associated ced-3 homolog) (MACH) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]

Bibliography :
[28336776] Apoptosis inhibitor 5 is an endogenous inhibitor of caspase-2.
[25405832] Influence of recombinant lentiviral vector encoding miR-15a/16-1 in biological features of human nasopharyngeal carcinoma CNE-2Z cells.
[25010987] Attenuation of the ELAV1-like protein HuR sensitizes adenocarcinoma cells to the intrinsic apoptotic pathway by increasing the translation of caspase-2L.
[24664246] An EGFR/HER2-Bispecific and enediyne-energized fusion protein shows high efficacy against esophageal cancer.
[24567095] Caspase-2 protocols.
[24309927] Caspase-2 promotes cytoskeleton protein degradation during apoptotic cell death.
[24016566] [Influence of recombinant lentiviral vector encoding miR-15a/16-1 in biological features of human nasopharyngeal carcinoma CNE-2Z cells].
[23494218] Analysis of mutation effects on PIDDosome core complex.
[23042294] IRE1α cleaves select microRNAs during ER stress to derepress translation of proapoptotic Caspase-2.
[22825847] Degradomics reveals that cleavage specificity profiles of caspase-2 and effector caspases are alike.