Product name : Caspase-3, human recombinant CASP3 Human
Catalog number : 1083-10
Quantity: 10 μg
Supplier name : Biovis
Data sheet : Ask more or other datasheet now !
Caspase-3, human recombinant CASP3 Human Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Caspase-3, human recombinant CASP3 Human.
FORM: Lyophilized powder
RECONSTITUTION: Reconstitute in PBS containing 15% glycerol.
The lyophilized caspase-3 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.
Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspasefamily of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme.
During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme (Full length gene Accession No. NP_004337) . The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat.#1008-200) or Ac-DEVD-AFC (1007-200).
APPLICATIONS AND USAGE:
Active caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays and Western blot analysis.
For a complete caspase-3 assay protocol, please refer to BioVision’s Caspase-3/CPP32 Fluorometric or Colorimetric Assay Kits (Cat.#: K105 and K106).
For Research Use Only! Not to be used in humans.
Apoptosis Detection Kits & Reagents
Cell Fractionation System
Cell Proliferation & Senescence
Cell Damage & Repair
Adipocyte & Lipid Transfer
Molecular Biology & Reporter Assays
Growth Factors and Cytokines
Monoclonal and Polyclonal Antibodies
Our team will respond you as soon as possible !
Kits Elisa; taq POLYMERASE
|WP1322: IL-6 Signaling Pathway|
|WP1530: miRNA regulation of DNA Damage Response|
|WP1772: Apoptosis Modulation and Signaling|
|WP2087: miRNA regulation of DNA Damage Response|
|WP2107: pathways in human cancer|
|WP387: IL-6 signaling Pathway|
|WP100: Glutathione metabolism|
|WP1019: FAS pathway and Stress induction of HSP regulation|
|WP1039: Glutathione metabolism|
|WP104: Alanine and aspartate metabolism|
|WP1047: TNF-alpha NF-kB Signaling Pathway|
|WP106: Alanine and aspartate metabolism|
|WP1082: Apoptosis Modulation by HSP70|
|WP1094: IL-6 Signaling Pathway|
|WP1103: Alpha6-Beta4 Integrin Signaling Pathway|
|WP1114: MAPK signaling pathway|
|WP1126: Tryptophan metabolism|
|WP1138: FAS pathway and Stress induction of HSP regulation|
|WP1163: TNF-alpha NF-kB Signaling Pathway|
|WP1199: Apoptosis Modulation by HSP70|
|WP1329: Alpha6-Beta4 Integrin Signaling Pathway|
|[CASP3 CPP32] Caspase-3 (CASP-3) (EC 188.8.131.52) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]|
|[Casp3 Cpp32] Caspase-3 (CASP-3) (EC 184.108.40.206) (Apopain) (Cysteine protease CPP32) (CPP-32) (LICE) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]|
|[Casp3 Cpp32] Caspase-3 (CASP-3) (EC 220.127.116.11) (Apopain) (Cysteine protease CPP32) (CPP-32) (IRP) (LICE) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]|
|[CASP3] Caspase 3|
|[CASP3 At2g27370 F10A12.5 F12K2.5] Casparian strip membrane protein 3 (AtCASP3)|
|[CASP3] Caspase 3 (Caspase-3-like protein)|
|[CASP3 CR201_G0037886] CASP3 isoform 1 (CASP3 isoform 2) (Caspase 3)|
|[CASP3] Caspase-3 (CASP-3) (EC 18.104.22.168) (Apopain) (Cysteine protease CPP32) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]|
|[CASP3 CK820_G0031735] CASP3 isoform 1 (CASP3 isoform 2) (Caspase 3, apoptosis-related cysteine peptidase)|
|[CASP7 MCH3] Caspase-7 (CASP-7) (EC 22.214.171.124) (Apoptotic protease Mch-3) (CMH-1) (ICE-like apoptotic protease 3) (ICE-LAP3) [Cleaved into: Caspase-7 subunit p20; Caspase-7 subunit p11]|
|[CASP8 MCH5] Caspase-8 (CASP-8) (EC 126.96.36.199) (Apoptotic cysteine protease) (Apoptotic protease Mch-5) (CAP4) (FADD-homologous ICE/ced-3-like protease) (FADD-like ICE) (FLICE) (ICE-like apoptotic protease 5) (MORT1-associated ced-3 homolog) (MACH) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]|
|[CASP9 MCH6] Caspase-9 (CASP-9) (EC 188.8.131.52) (Apoptotic protease Mch-6) (Apoptotic protease-activating factor 3) (APAF-3) (ICE-like apoptotic protease 6) (ICE-LAP6) [Cleaved into: Caspase-9 subunit p35; Caspase-9 subunit p10]|
|[CASP6 MCH2] Caspase-6 (CASP-6) (EC 184.108.40.206) (Apoptotic protease Mch-2) [Cleaved into: Caspase-6 subunit p18; Caspase-6 subunit p11]|
|[CASP3 QmoA-14231] Caspase-3 (CASP-3) (EC 220.127.116.11) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]|
|[TXN TRDX TRX TRX1] Thioredoxin (Trx) (ATL-derived factor) (ADF) (Surface-associated sulphydryl protein) (SASP)|
|[XIAP API3 BIRC4 IAP3] E3 ubiquitin-protein ligase XIAP (EC 18.104.22.168) (Baculoviral IAP repeat-containing protein 4) (IAP-like protein) (ILP) (hILP) (Inhibitor of apoptosis protein 3) (IAP-3) (hIAP-3) (hIAP3) (RING-type E3 ubiquitin transferase XIAP) (X-linked inhibitor of apoptosis protein) (X-linked IAP)|
|[CASP3] Caspase 3|
|[ATG4D APG4D AUTL4] Cysteine protease ATG4D (EC 3.4.22.-) (AUT-like 4 cysteine endopeptidase) (Autophagin-4) (Autophagy-related cysteine endopeptidase 4) (Autophagy-related protein 4 homolog D) [Cleaved into: Cysteine protease ATG4D, mitochondrial]|
|[CASP3] Caspase 3|
| Molecular characterization and antiapoptotic function analysis of the duck plague virus Us5 gene.|
| [Targeted binding of estradiol with ESR1 promotes proliferation of human chondrocytes by inhibiting activation of ERK signaling pathway].|
| [Construction of 3β-HSD gene silencing cell line and its effects on apoptosis induced by DEHP].|
| Manilkara zapota (L.) P. Royen Leaf Extract Derived Silver Nanoparticles Induce Apoptosis in Human Colorectal Carcinoma Cells Without Affecting Human Lymphocytes or Erythrocytes.|
| Recombinant Human Superoxide Dismutase and -Acetylcysteine Addition to Exogenous Surfactant in the Treatment of Meconium Aspiration Syndrome.|
| Modification of IL-24 by tumor penetrating peptide iRGD enhanced its antitumor efficacy against non-small cell lung cancer.|
| Growth arrest and DNA damage 45γ is required for caspase-dependent renal tubular cell apoptosis.|
| Activation of CD3 T cells by Helicobacter pylori DNA vaccines in potential immunotherapy of gastric carcinoma.|
| Tumor necrosis factor-related apoptosis inducing ligand induces apoptosis in canine hemangiosarcoma cells in vitro.|
| Chemerin reverses neurological impairments and ameliorates neuronal apoptosis through ChemR23/CAMKK2/AMPK pathway in neonatal hypoxic-ischemic encephalopathy.|