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Index / Biovis / Caspase-3, mouse recombinant CASP3 Mouse /Product Detail : 1183-25 Caspase-3, mouse recombinant CASP3 Mouse
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#1183-25 Caspase-3, mouse recombinant CASP3 Mouse

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  Price : 219   EUR
248   USD
170   GBP
921   Zloty
29343   JPY
1691   NOK
1812   SEK
248   CHF

Product name : Caspase-3, mouse recombinant CASP3 Mouse

Catalog number : 1183-25

Quantity: 25 units

Availability: Yes

Supplier name : Biovis

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About this Product :

Caspase-3, mouse recombinant CASP3 Mouse recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. Please read carefully the data sheet of the Caspase-3, mouse recombinant CASP3 Mouse.
http://mybiofast.com/ver.php?search=rec.&submit=Search

Caspase-3, mouse recombinant CASP3 Mouse mus musculus murine Caspase-3, mouse recombinant CASP3 Mouse detects proteins from variouse species most likely human.
http://mybiofast.com/ver.php?search=musculus&submit=Search

More Details about

UNIT DEFINATION:
One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.


FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 300,000 units/mg


STORAGE CONDITIONS:
The lyophilized caspase-3 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.


DESCRIPTION:
Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspasefamily of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme.
During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat. #1008-200) or Ac-DEVD-AFC (1007-200).


APPLICATIONS AND USAGE:
Active caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase-3 assay protocol, please refer to BioVision’s Caspase-3/CPP32 Fluorometric or Colorimetric Assay Kits (Cat.#: K105 and K106).

 

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