Product name : Caspase-5, human recombinant CASP5 Human
Catalog number : 1085-100
Quantity: 100 units
Supplier name : Biovis
Data sheet : Ask more or other datasheet now !
Caspase-5, human recombinant CASP5 Human Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and elisa kit to Human proteins as well as Caspase-5, human recombinant CASP5 Human.
One unit of the recombinant caspase-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 5000 units/mg
PURITY: >95% by SDS-PAGE
The lyophilized caspase-5 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.
Caspase-5 (also know as ICErelIII, TY) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-5 exists in cells as an inactive proenzyme which is matured by proteolysis to yield large and small subunits. The active caspase-5 is a heterotetramer consisting of two large and two small subunits. To date the mechanism of caspase-5 activation and roles of caspase-5 in apoptosis are poorly understood.
The recombinant active human caspase-5 was expressed in E. coli. The active caspase-5 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-WEHD-pNA (Cat. #1102-200) or Ac-WEHD-AFC (1101-200).
APPLICATIONS AND USAGE:
Active caspase-5 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase-5 assay protocol, please refer to BioVision’s Caspase-5 Fluorometric or Colorimetric Assay Kits (Cat.#: K122 and K123).
For Research Use Only! Not to be used in humans.
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