Product name : Caspase-8, human recombinant CASP8 Human
Catalog number : 1088-100
Supplier name : Biovis
Data sheet : Ask more or other datasheet now !
Caspase-8, human recombinant CASP8 Human Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Caspase-8, human recombinant CASP8 Human.
One unit of the recombinant caspase-8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 5000 units/mg
PURITY: >95% by SDS-PAGE
The lyophilized caspase-8 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.
Caspase-8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by selfproteolysis and/or cleavage by another caspase. The processed active form of caspase-8 consists of large and small subunits which associate to form the active enzyme. Active caspase-8 has been shown to activate caspase-3 leading to degradation of a variety of cellular target proteins during apoptosis.
The recombinant active human caspase-8 was expressed in E. coli. The active caspase-8 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).
APPLICATIONS AND USAGE:
Active caspase-8 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase-8 assay protocol, please refer to BioVision’s Caspase-8 Fluorometric or Colorimetric Assay Kits (Cat.#: K112 and K113).
For Research Use Only! Not to be used in humans.
Apoptosis Detection Kits & Reagents
Cell Fractionation System
Cell Proliferation & Senescence
Cell Damage & Repair
Adipocyte & Lipid Transfer
Molecular Biology & Reporter Assays
Growth Factors and Cytokines
Monoclonal and Polyclonal Antibodies
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Kits Elisa; taq POLYMERASE
|WP1772: Apoptosis Modulation and Signaling|
|WP2107: pathways in human cancer|
|WP1019: FAS pathway and Stress induction of HSP regulation|
|WP1047: TNF-alpha NF-kB Signaling Pathway|
|WP1067: Toll-like receptor signaling pathway|
|WP1082: Apoptosis Modulation by HSP70|
|WP1114: MAPK signaling pathway|
|WP1138: FAS pathway and Stress induction of HSP regulation|
|WP1163: TNF-alpha NF-kB Signaling Pathway|
|WP1183: Toll-like receptor signaling pathway|
|WP1199: Apoptosis Modulation by HSP70|
|WP1271: Toll-like receptor signaling pathway|
|WP1309: Toll-like receptor signaling pathway|
|WP1337: MAPK signaling pathway|
|WP1348: Androgen Receptor Signaling Pathway|
|WP1369: TNF-alpha NF-kB Signaling Pathway|
|WP1384: Toll-like receptor signaling pathway|
|WP1392: Apoptosis Modulation by HSP70|
|WP1433: NOD pathway|
|WP1449: Regulation of toll-like receptor signaling pathway|
|[CFLAR CASH CASP8AP1 CLARP MRIT] CASP8 and FADD-like apoptosis regulator (Caspase homolog) (CASH) (Caspase-eight-related protein) (Casper) (Caspase-like apoptosis regulatory protein) (CLARP) (Cellular FLICE-like inhibitory protein) (c-FLIP) (FADD-like antiapoptotic molecule 1) (FLAME-1) (Inhibitor of FLICE) (I-FLICE) (MACH-related inducer of toxicity) (MRIT) (Usurpin) [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]|
|[CASP8 MCH5] Caspase-8 (CASP-8) (EC 184.108.40.206) (Apoptotic cysteine protease) (Apoptotic protease Mch-5) (CAP4) (FADD-homologous ICE/ced-3-like protease) (FADD-like ICE) (FLICE) (ICE-like apoptotic protease 5) (MORT1-associated ced-3 homolog) (MACH) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]|
|[Cflar Cash] CASP8 and FADD-like apoptosis regulator (Caspase homolog) (CASH) (Caspase-eight-related protein) (Casper) (Caspase-like apoptosis regulatory protein) (CLARP) (Cellular FLICE-like inhibitory protein) (c-FLIP) (FADD-like antiapoptotic molecule 1) (FLAME-1) (Inhibitor of FLICE) (I-FLICE) (MACH-related inducer of toxicity) (MRIT) (Usurpin) [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]|
|[Casp8] Caspase-8 (CASP-8) (EC 220.127.116.11) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]|
|[Casp8] Caspase-8 (CASP-8) (EC 18.104.22.168) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]|
|[CASP8AP2 FLASH KIAA1315 RIP25] CASP8-associated protein 2 (FLICE-associated huge protein)|
|[Casp8ap2 Flash] CASP8-associated protein 2 (FLICE-associated huge protein)|
|[Cflar rCG_22405] CASP8 and FADD-like apoptosis regulator (CASP8 and FADD-like apoptosis regulator, isoform CRA_a) (Cflar protein)|
|[RNF34] E3 ubiquitin-protein ligase RNF34 (EC 22.214.171.124) (Caspase regulator CARP1) (Caspases-8 and -10-associated RING finger protein 1) (CARP-1) (FYVE-RING finger protein Momo) (Human RING finger homologous to inhibitor of apoptosis protein) (hRFI) (RING finger protein 34) (RING finger protein RIFF) (RING-type E3 ubiquitin transferase RNF34)|
|[CASP10 MCH4] Caspase-10 (CASP-10) (EC 126.96.36.199) (Apoptotic protease Mch-4) (FAS-associated death domain protein interleukin-1B-converting enzyme 2) (FLICE2) (ICE-like apoptotic protease 4) [Cleaved into: Caspase-10 subunit p23/17; Caspase-10 subunit p12]|
|[RFFL RNF189 RNF34L] E3 ubiquitin-protein ligase rififylin (EC 188.8.131.52) (Caspase regulator CARP2) (Caspases-8 and -10-associated RING finger protein 2) (CARP-2) (FYVE-RING finger protein Sakura) (Fring) (RING finger and FYVE-like domain-containing protein 1) (RING finger protein 189) (RING finger protein 34-like) (RING-type E3 ubiquitin transferase rififylin)|
|[PYCARD ASC CARD5 TMS1] Apoptosis-associated speck-like protein containing a CARD (hASC) (Caspase recruitment domain-containing protein 5) (PYD and CARD domain-containing protein) (Target of methylation-induced silencing 1)|
|[FADD MORT1 GIG3] FAS-associated death domain protein (FAS-associating death domain-containing protein) (Growth-inhibiting gene 3 protein) (Mediator of receptor induced toxicity) (Protein FADD)|
|[NOL3 ARC NOP] Nucleolar protein 3 (Apoptosis repressor with CARD) (Muscle-enriched cytoplasmic protein) (Myp) (Nucleolar protein of 30 kDa) (Nop30)|
|[CASP8 CK820_G0014830] CASP8 isoform 11 (Caspase 8, apoptosis-related cysteine peptidase)|
|[BCAP31 BAP31 DXS1357E] B-cell receptor-associated protein 31 (BCR-associated protein 31) (Bap31) (6C6-AG tumor-associated antigen) (Protein CDM) (p28)|
|[IFT57 DERP8 ESRRBL1 HIPPI] Intraflagellar transport protein 57 homolog (Dermal papilla-derived protein 8) (Estrogen-related receptor beta-like protein 1) (HIP1-interacting protein) (MHS4R2)|
|[TRAF2 TRAP3] TNF receptor-associated factor 2 (EC 184.108.40.206) (E3 ubiquitin-protein ligase TRAF2) (RING-type E3 ubiquitin transferase TRAF2) (Tumor necrosis factor type 2 receptor-associated protein 3)|
|[TRAF1 EBI6] TNF receptor-associated factor 1 (Epstein-Barr virus-induced protein 6)|
|[ATG3 APG3 APG3L] Ubiquitin-like-conjugating enzyme ATG3 (EC 2.3.2.-) (Autophagy-related protein 3) (APG3-like) (hApg3) (Protein PC3-96)|
|[BECN1 GT197] Beclin-1 (Coiled-coil myosin-like BCL2-interacting protein) (Protein GT197) [Cleaved into: Beclin-1-C 35 kDa; Beclin-1-C 37 kDa]|
|[CFLAR hCG_16553] CASP8 and FADD-like apoptosis regulator, isoform CRA_c (Testis secretory sperm-binding protein Li 233m)|
|[XIAP API3 BIRC4 IAP3] E3 ubiquitin-protein ligase XIAP (EC 220.127.116.11) (Baculoviral IAP repeat-containing protein 4) (IAP-like protein) (ILP) (hILP) (Inhibitor of apoptosis protein 3) (IAP-3) (hIAP-3) (hIAP3) (RING-type E3 ubiquitin transferase XIAP) (X-linked inhibitor of apoptosis protein) (X-linked IAP)|
|[RIPK1 RIP RIP1] Receptor-interacting serine/threonine-protein kinase 1 (EC 18.104.22.168) (Cell death protein RIP) (Receptor-interacting protein 1) (RIP-1)|
|[CDK1 CDC2 CDC28A CDKN1 P34CDC2] Cyclin-dependent kinase 1 (CDK1) (EC 22.214.171.124) (EC 126.96.36.199) (Cell division control protein 2 homolog) (Cell division protein kinase 1) (p34 protein kinase)|
|[FAS APT1 FAS1 TNFRSF6] Tumor necrosis factor receptor superfamily member 6 (Apo-1 antigen) (Apoptosis-mediating surface antigen FAS) (FASLG receptor) (CD antigen CD95)|
|[BIRC2 API1 MIHB RNF48] Baculoviral IAP repeat-containing protein 2 (EC 188.8.131.52) (Cellular inhibitor of apoptosis 1) (C-IAP1) (IAP homolog B) (Inhibitor of apoptosis protein 2) (hIAP-2) (hIAP2) (RING finger protein 48) (RING-type E3 ubiquitin transferase BIRC2) (TNFR2-TRAF-signaling complex protein 2)|
|[TNFRSF10A APO2 DR4 TRAILR1] Tumor necrosis factor receptor superfamily member 10A (Death receptor 4) (TNF-related apoptosis-inducing ligand receptor 1) (TRAIL receptor 1) (TRAIL-R1) (CD antigen CD261)|
|[Cflar rCG_22405] CASP8 and FADD-like apoptosis regulator, isoform CRA_b (FLIP short form)|
|[CASP8] Caspase 8|
| The caspase-8/Bid/cytochrome c axis links signals from death receptors to mitochondrial reactive oxygen species production.|
| The functional domains for Bax∆2 aggregate-mediated caspase 8-dependent cell death.|
| Enhancement of death receptor 4-mediated apoptosis and cytotoxicity in renal cell carcinoma cells by anisomycin.|
| Cryo-EM Structure of Caspase-8 Tandem DED Filament Reveals Assembly and Regulation Mechanisms of the Death-Inducing Signaling Complex.|
| TRAIL-coated lipid-nanoparticles overcome resistance to soluble recombinant TRAIL in non-small cell lung cancer cells.|
| Enzymatically active cathepsin D sensitizes breast carcinoma cells to TRAIL.|
| A novel caspase 8 selective small molecule potentiates TRAIL-induced cell death.|
| TNF-related apoptosis-inducing ligand promotes human preadipocyte proliferation via ERK1/2 activation.|
| [The synergism and mechanism of action of rClone30-hDR5 in combination with TRAIL on HCC].|
| The caspase-8 shRNA-modified mesenchymal stem cells improve the function of infarcted heart.|