Product name : Caspase-8, mouse recombinant CASP8 Mouse
Catalog number : 1188-25
Quantity: 25 units
Supplier name : Biovis
Data sheet : Ask more or other datasheet now !
Caspase-8, mouse recombinant CASP8 Mouse mus musculus murine Caspase-8, mouse recombinant CASP8 Mouse detects proteins from variouse species most likely human.
One unit of the recombinant caspase-8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 5000 units/mg
PURITY: >95% by SDS-PAGE
The lyophilized caspase-8 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.
Caspase-8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by selfproteolysis and/or cleavage by another caspase. The processed active form of caspase-8 consists of large and small subunits which associate to form the active enzyme. Active caspase-8 has been shown to activate caspase-3 leading to degradation of a variety of cellular target proteins during apoptosis.
The recombinant active mouse caspase-8 was expressed in E. coli. The active caspase-8 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).
APPLICATIONS AND USAGE:
Active caspase-8 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase-8 assay protocol, please refer to BioVision’s Caspase-8 Fluorometric or Colorimetric Assay Kits (Cat.#: K112 and K113).
FOR RESEARCH USE ONLY! Not to be used in humans.
Apoptosis Detection Kits & Reagents
Cell Fractionation System
Cell Proliferation & Senescence
Cell Damage & Repair
Adipocyte & Lipid Transfer
Molecular Biology & Reporter Assays
Growth Factors and Cytokines
Monoclonal and Polyclonal Antibodies
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Kits Elisa; taq POLYMERASE
|WP1019: FAS pathway and Stress induction of HSP regulation|
|WP1047: TNF-alpha NF-kB Signaling Pathway|
|WP1067: Toll-like receptor signaling pathway|
|WP1082: Apoptosis Modulation by HSP70|
|WP1114: MAPK signaling pathway|
|WP1138: FAS pathway and Stress induction of HSP regulation|
|WP1163: TNF-alpha NF-kB Signaling Pathway|
|WP1183: Toll-like receptor signaling pathway|
|WP1199: Apoptosis Modulation by HSP70|
|WP1271: Toll-like receptor signaling pathway|
|WP1309: Toll-like receptor signaling pathway|
|WP1337: MAPK signaling pathway|
|WP1348: Androgen Receptor Signaling Pathway|
|WP1369: TNF-alpha NF-kB Signaling Pathway|
|WP1384: Toll-like receptor signaling pathway|
|WP1392: Apoptosis Modulation by HSP70|
|WP1433: NOD pathway|
|WP1449: Regulation of toll-like receptor signaling pathway|
|WP1530: miRNA regulation of DNA Damage Response|
|WP1579: DNA damage response|
|[Cflar Cash] CASP8 and FADD-like apoptosis regulator (Caspase homolog) (CASH) (Caspase-eight-related protein) (Casper) (Caspase-like apoptosis regulatory protein) (CLARP) (Cellular FLICE-like inhibitory protein) (c-FLIP) (FADD-like antiapoptotic molecule 1) (FLAME-1) (Inhibitor of FLICE) (I-FLICE) (MACH-related inducer of toxicity) (MRIT) (Usurpin) [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]|
|[Casp8] Caspase-8 (CASP-8) (EC 18.104.22.168) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]|
|[CFLAR CASH CASP8AP1 CLARP MRIT] CASP8 and FADD-like apoptosis regulator (Caspase homolog) (CASH) (Caspase-eight-related protein) (Casper) (Caspase-like apoptosis regulatory protein) (CLARP) (Cellular FLICE-like inhibitory protein) (c-FLIP) (FADD-like antiapoptotic molecule 1) (FLAME-1) (Inhibitor of FLICE) (I-FLICE) (MACH-related inducer of toxicity) (MRIT) (Usurpin) [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]|
|[CASP8 MCH5] Caspase-8 (CASP-8) (EC 22.214.171.124) (Apoptotic cysteine protease) (Apoptotic protease Mch-5) (CAP4) (FADD-homologous ICE/ced-3-like protease) (FADD-like ICE) (FLICE) (ICE-like apoptotic protease 5) (MORT1-associated ced-3 homolog) (MACH) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10]|
|[Casp8ap2 Flash] CASP8-associated protein 2 (FLICE-associated huge protein)|
|[CASP8AP2 FLASH KIAA1315 RIP25] CASP8-associated protein 2 (FLICE-associated huge protein)|
|[Cflar rCG_22405] CASP8 and FADD-like apoptosis regulator (CASP8 and FADD-like apoptosis regulator, isoform CRA_a) (Cflar protein)|
|[Nol3 Arc] Nucleolar protein 3 (Apoptosis repressor with CARD)|
|[Pycard Asc] Apoptosis-associated speck-like protein containing a CARD (mASC) (PYD and CARD domain-containing protein)|
|[Traf2] TNF receptor-associated factor 2 (EC 126.96.36.199) (E3 ubiquitin-protein ligase TRAF2) (RING-type E3 ubiquitin transferase TRAF2)|
|[Xiap Aipa Api3 Birc4 Miha] E3 ubiquitin-protein ligase XIAP (EC 188.8.131.52) (Baculoviral IAP repeat-containing protein 4) (IAP homolog A) (Inhibitor of apoptosis protein 3) (IAP-3) (mIAP-3) (mIAP3) (RING-type E3 ubiquitin transferase XIAP) (X-linked inhibitor of apoptosis protein) (X-linked IAP)|
|[Rffl] E3 ubiquitin-protein ligase rififylin (EC 184.108.40.206) (RING finger and FYVE-like domain-containing protein 1) (Fring) (RING-type E3 ubiquitin transferase rififylin)|
|[Fadd Mort1] FAS-associated death domain protein (FAS-associating death domain-containing protein) (Mediator of receptor induced toxicity) (Protein FADD)|
|[Ift57 Hippi] Intraflagellar transport protein 57 homolog (HIP1-interacting protein)|
|[Bcap31 Bap31] B-cell receptor-associated protein 31 (BCR-associated protein 31) (Bap31) (p28)|
|[Fas Apt1 Tnfrsf6] Tumor necrosis factor receptor superfamily member 6 (Apo-1 antigen) (Apoptosis-mediating surface antigen FAS) (FASLG receptor) (CD antigen CD95)|
|[Birc2] Baculoviral IAP repeat-containing protein 2 (EC 220.127.116.11) (Cellular inhibitor of apoptosis 1) (C-IAP1) (Inhibitor of apoptosis protein 2) (mIAP2) (RING-type E3 ubiquitin transferase BIRC2)|
|[Csnk2a1 Ckiia] Casein kinase II subunit alpha (CK II alpha) (EC 18.104.22.168)|
|[Birc3] Baculoviral IAP repeat-containing protein 3 (EC 22.214.171.124) (Cellular inhibitor of apoptosis 2) (C-IAP2) (Inhibitor of apoptosis protein 1) (mIAP1) (RING-type E3 ubiquitin transferase BIRC3)|
|[Pea15 Pea15a] Astrocytic phosphoprotein PEA-15 (15 kDa phosphoprotein enriched in astrocytes)|
|[Atg3 Apg3l] Ubiquitin-like-conjugating enzyme ATG3 (EC 2.3.2.-) (Autophagy-related protein 3) (APG3-like)|
|[CASP8 CK820_G0014830] CASP8 isoform 11 (Caspase 8, apoptosis-related cysteine peptidase)|
|[Rnf34] E3 ubiquitin-protein ligase RNF34 (EC 126.96.36.199) (Phafin-1) (RING finger protein 34) (RING-type E3 ubiquitin transferase RNF34)|
|[Tradd] Tumor necrosis factor receptor type 1-associated DEATH domain protein (TNFR1-associated DEATH domain protein) (TNFRSF1A-associated via death domain)|
|[Cflar mCG_117843] CASP8 and FADD-like apoptosis regulator (CASP8 and FADD-like apoptosis regulator, isoform CRA_a)|
|[Cdk1 Cdc2 Cdc2a Cdkn1] Cyclin-dependent kinase 1 (CDK1) (EC 188.8.131.52) (EC 184.108.40.206) (Cell division control protein 2 homolog) (Cell division protein kinase 1) (p34 protein kinase)|
|[Csnk2a2] Casein kinase II subunit alpha' (CK II alpha') (EC 220.127.116.11)|
|[CASP10 MCH4] Caspase-10 (CASP-10) (EC 18.104.22.168) (Apoptotic protease Mch-4) (FAS-associated death domain protein interleukin-1B-converting enzyme 2) (FLICE2) (ICE-like apoptotic protease 4) [Cleaved into: Caspase-10 subunit p23/17; Caspase-10 subunit p12]|
|[Traf1] TNF receptor-associated factor 1|
| A novel Fas-binding outer membrane protein and lipopolysaccharide of Leptospira interrogans induce macrophage apoptosis through the Fas/FasL-caspase-8/-3 pathway.|
| The PGRS Domain of Mycobacterium tuberculosis PE_PGRS Protein Rv0297 Is Involved in Endoplasmic Reticulum Stress-Mediated Apoptosis through Toll-Like Receptor 4.|
| Soluble Fas Ligand Is Essential for Blister Formation in Pemphigus.|
| The effects of interleukin 2 and rAd-p53 as a treatment for glioblastoma.|
| The caspase-8/Bid/cytochrome c axis links signals from death receptors to mitochondrial reactive oxygen species production.|
| Effects of the Notch1 signaling pathway on human lung cancer A549 cells.|
| Plumbagin enhances TRAIL-induced apoptosis of human leukemic Kasumi‑1 cells through upregulation of TRAIL death receptor expression, activation of caspase-8 and inhibition of cFLIP.|
| Recombinant human brain-derived neurotrophic factor prevents neuronal apoptosis in a novel in vitro model of subarachnoid hemorrhage.|
| Amphiregulin suppresses epithelial cell apoptosis in lipopolysaccharide-induced lung injury in mice.|
| Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways.|