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PARP monoclonal Antibody Monoclonal Host: Mouse 10H
/Product Detail : 3000-100 PARP monoclonal Antibody Monoclonal Host: Mouse 10H
Product name : PARP monoclonal Antibody Monoclonal Host: Mouse 10H
Catalog number : 3000-100
Supplier name : Biovis
Data sheet : Ask more or other datasheet now !
PARP monoclonal Antibody Monoclonal Host: Mouse 10H mus musculus murine PARP monoclonal Antibody Monoclonal Host: Mouse 10H detects proteins from variouse species most likely human.
PARP monoclonal Antibody Monoclonal Host: Mouse 10H http://biotech-map.com/aa_search/table_sample.html?q=clone&filter=anti
Storage and handling
Antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
Antibody Target: PARP
Alternative Name: PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1
Antibody Type: Monoclonal (Clone 10H)
Isotype: Mouse IgG3κ
Immunogen: Poly(ADP-ribose) mixed with methylated bovine serum albumin
Gene ID: 142
Appearance: Colorless liquid
Concentration: 1.0 mg/ml
Formulation: 1 mg/ml of IgG purified from mouse ascites by protein A chromatography. Prepared in 20 mM Tris, pH 7.4, 150 mM NaCl, 1% BSA and 0.02% sodium azide.
Species Reactivity: All
Application: Western blot, Immunoprecipitation, Immunocytochemistry & ELISA
Application & Usage: Immunocytochemistry : 5-20 μg/ml; Western Blot (10 μg/ml using colorimetric methods; <2 μg/ml for ECL); Immunoprecipitation and ELISA
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans.
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