"The MyTaq HS sample was so much better than anything else we have used, we have ordered more already."
- Roslin Institute, Scotland
New generation of antibody-based hot-start polymerase
Highest specificity and superior performance
Novel buffer system, including dNTPs and MgCl2
Fast PCR reactions
Assays with prolonged reaction setup on the bench or liquid handling
Amplification of challenging targets susceptible to mispriming
Amplification from challenging plant samples
Specific amplification of difficult templates (GC rich)
MyTaq™ HS DNA Polymerase is a high performance PCR product powered by antibody mediated hot-start, specifically designed for fast, highly-specific, hot-start PCR. The product also has the added convenience of room temperature reaction assembly, without non-specific amplification and primer-dimer formation. This new hot-start enzyme preparation from Bioline is supplied with 5x MyTaq Reaction Buffer, a proprietary formulation containing dNTPs, MgCl2 and enhancers at optimal concentrations, removing the need for optimization and giving superior amplification (Fig. 1).
MyTaq HS DNA Polymerase
5x MyTaq Reaction Buffer
One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.
MyTaq HS DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.
Murray, S.A., et al. Appl. Envir. Microbiol. 77, 7050-7057(2011).