Higher-fidelity, specifically developed for TA cloning
Novel antibody-based hot-start polymerase
Amplifies genomic DNA fragments up to 10kb
Industry-leading novel buffer system
Available as an all-in-one master mix
Specifically developed for TA cloning
PCR requiring high specificity combined with higher fidelity
Good for low copy PCR assays
MyFi™ is a newly developed, antibody-mediated, hot-start enzyme with unique properties, that offers 3.5x higher fidelity than native Taq and enhanced sensitivity and specificity, making MyFi a superior choice for cloning. The polymerase is supplied with MyFi Buffer, a highly optimized proprietary formulation, containing ultra-pure dNTPs, MgCl2 and enhancers, specifically formulated and validated to provide outstanding results.
MyFi is ideally suited for difficult PCR amplification and cloning of targets with variable lengths up to 10kb, for example, amplification of cDNA libraries, complex genomic fragments (Fig. 1), targets with high GC-content (Fig. 2) and low-copy assays which require both high processivity and higher fidelity. MyFi has the added convenience of room temperature reaction assembly, to avoid non-specific amplification and primer-dimer formation. MyFi generates new products with 3'-A overhangs, perfect for TA cloning.
One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.
MyFi DNA Polymerase
5x MyFi Reaction Buffer
BIO-21117: 250 x 25µl Reactions: 1 x 125µl
BIO-21118: 500 x 25µl Reactions: 1 x 250µl
BIO-21119: 2500 x 25µl Reactions: 2 x 625µl
MyFi DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.