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Index / Biolin / DNA Polymerases - For Proof-Reading_Difficult Applications : BIO-X-ACT Short DNA Polymerase /Product Detail : BIO-21064 DNA Polymerases - For Proof-Reading_Difficult Applications : BIO-X-ACT Short DNA Polymerase
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DNA Polymerases - For Proof-Reading_Difficult Applications : BIO-X-ACT Short DNA Polymerase

 Price: 188   EUR
213   USD
146   GBP

Product name : DNA Polymerases - For Proof-Reading_Difficult Applications : BIO-X-ACT Short DNA Polymerase

Catalog number : BIO-21064

Quantity: 250 Units

Availability: Yes

Supplier name : Biolin

Data sheet Data sheet : Ask more or other datasheet now !

More Details about

Features
 
  • Ideal for problematic templates
  • Ideal for templates that fail with standard Taq DNA Polymerases
  • Higher fidelity than Taq due to proofreading activity
  • Amplifies fragments up to 5Kb
  • Available as a ready-to-use 2x reaction mix (BIO-X-ACT™ Short Mix)

 
Applications
 
  • High-fidelity PCR
  • Suitable for TA cloning
  • GC-rich templates
  • Crude sample PCR
  • Forensic applications

 
Description

BIO-X-ACT™ Short DNA Polymerase is a high-performance enzyme designed for difficult or problematic PCR applications requiring high processivity and fidelity. Two versions of BIO-X-ACT are available, for amplification of short and long DNA fragments respectively, and are the polymerases of choice for difficult applications that would normally fail with standard Taq polymerases.

BIO-X-ACT Short is recommended for short genomic DNA fragments of up to 2Kb, or Lambda DNA fragments up to 5Kb. Using Lambda DNA as template, the best performance is achieved within the 100bp-3Kb range.

For enhanced specificity, BIO-X-ACT Short is supplied with a vial of 5x Hi-Spec Additive. Hi-Spec Additive is a very efficient enhancer, which helps to prevent the formation of false background bands and smearing. The specificity and performance can be further improved with the use of 2x PolyMate Additive (not supplied, see associated products), which is designed for GC or AT-rich DNA, "dirty" templates or sequences with difficult melting profiles.

 
Unit Definition

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
 
Concentration

4u/µl
 
Storage Conditions

BIO-X-ACT Short DNA Polymerase can be stored for 12 months at -20°C.
 
Shipping Conditions

On Dry Ice or Blue Ice.
 
Product Citations
 
  1. Blake, D.P., et al. PLoS Pathog. 7(2), e1001279 (2011).
  2. Kuperman, Y. et al. Mol. Endocrinol. 25 157-169 (2011).
  3. Li, H., et al. J. Bio. Chem. 286, 21500-21510 (2011).
  4. Taliaz, D., et al. J. Neurosci. 31 4475-4483 (2011).
  5. Boura-Halfon, S., et al. Mol. Endocrinol. 24 2179-2192 (2010).
  6. David, M., et al. RNA 16 364-374 (2010).
  7. Falvella, F. S., et al. J. Natl. Can. Inst. 102(17), 1367-1370. (2010).
  8. Petersen, H. J., et al. Infect. Immun. 78, 413-422 (2010).
  9. Matic, M., et al. Intl. J. Biochem. Cell Biol. 42(5), 672-682 (2010).
  10. Lim, T. S., et al. New Biotechnol. 27(2), 108-117 (2010).
  11. Solomon, E., et al. J. Bio. Chem. 285, 21969-77 (2010).
  12. Hedman, J., et al. BioTechniques 47, 951-958 (2009).
  13. Dhivya, K. & Ghosh, G. J. Forensic Dental Sci. 1(2), 104-106 (2009).

 

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