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Index / Biolin / DNA Polymerases - For High-Fidelity Applications : ACCUZYME DNA Polymerase /Product Detail : BIO-21051 DNA Polymerases - For High-Fidelity Applications : ACCUZYME DNA Polymerase
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DNA Polymerases - For High-Fidelity Applications : ACCUZYME DNA Polymerase

 Price: 201   EUR
228   USD
156   GBP

Product name : DNA Polymerases - For High-Fidelity Applications : ACCUZYME DNA Polymerase

Catalog number : BIO-21051

Quantity: 250 Units

Availability: Yes

Supplier name : Biolin

Data sheet Data sheet : Ask more or other datasheet now !

More Details about

 
 
Features
 
  • High-fidelity coupled with high yield
  • Amplifies fragments up to 5 Kb
  • Available as a convenient pre-mixed, pre-optimized solution (ACCUZYME™ Mix)

 
Applications
 
  • Ultra-high fidelity for subsequent cloning
  • Blunt-end cloning

 
Description

ACCUZYME™ is a thermostable enzyme possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities, offering high fidelity. ACCUZYME provides high fidelity with an error-rate of 3.0 x 10-6, to give blunt-ended amplicons of up to 5Kb in length.

ACCUZYME is supplied with 10x Reaction Buffer containing MgSO4, which provides optimal final reaction conditions (1.5mM Mg2+) for most experiments. In order to allow optimization of reaction conditions, additional MgCl2 is provided.

The specificity and performance of ACCUZYME can be further improved with the use of 2x PolyMate Additive, which is designed for GC or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.

 
Unit Definition

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
 
Concentration

2.5u/µl
 
Storage Conditions

ACCUZYME can be stored for 12 months at -20°C.
 
Shipping Conditions

On Dry Ice or Blue Ice
 
Product Citations
 
  1. Kitazono, A.A., Gene doi:10.1016/j.gene.2011.06.006 (2011).
  2. Batchelor, D.J., et al. Am. J. Physiol. 300, R67-R75 (2011).
  3. Chiang, C., et al. J. Bacteriol. 193, 52-62 (2011).
  4. Lim, C.G., et al. Appl. Envir. Microbiol. 77, 3451-3460 (2011).
  5. Cheng, C., et al. Mol. Cell. Biol. 31, 983-997 (2011).
  6. Chakrabarti, M., et al. Virol. J. 7, 181 (2010).
  7. Silvestrini, F., et al. Mol.Cell. Prot. 9, 1437-48 (2010).

 

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